-
1.发明申请
公开(公告)号：WO2014062717A1
公开(公告)日：2014-04-24
申请号：PCT/US2013/065108
申请日：2013-10-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： ANDERSEN, Mark , ROMAN, Steven
CPC classification number: C12Q1/6806 , C07H21/00 , C12Q1/6853 , C12Q2525/301 , C12Q2527/107 , C12Q2563/131 , C12Q2563/149
Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating one or more target nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the amount of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture using a primer having a first primer sequence and a second primer sequence that are complementary at a first melting temperature and are not complementary at a second melting temperature. In some aspects, target nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and template library preparation.
Abstract translation: 本发明提供了可用于从样品中分离一种或多种靶核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的量。 一方面，本发明涉及使用具有第一引物序列和第二引物序列的引物从引物延伸反应混合物纯化引物延伸产物，所述第一引物序列和第二引物序列在第一熔融温度下互补且在第二熔融温度下不互补。 在一些方面，使用公开的方法，试剂盒，系统和装置获得的靶核酸分子可以用于包括核酸测序和模板文库制备在内的各种下游过程。
-
2.发明公开COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT 有权ZUSAMMENSETZUNGEN，VERFAHREN，SYSTEME UND KITS ZURZIELNUKLEINSÄUREANREICHERUNG
公开(公告)号：EP2906715A1
公开(公告)日：2015-08-19
申请号：EP13783775.3
申请日：2013-10-15
Applicant: Life Technologies Corporation
Inventor： ANDERSEN, Mark , ROMAN, Steven
CPC classification number: C12Q1/6806 , C07H21/00 , C12Q1/6853 , C12Q2525/301 , C12Q2527/107 , C12Q2563/131 , C12Q2563/149
Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating one or more target nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the amount of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture using a primer having a first primer sequence and a second primer sequence that are complementary at a first melting temperature and are not complementary at a second melting temperature. In some aspects, target nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and template library preparation.
Abstract translation: 本发明提供了可用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用公开的方法，试剂盒，系统和装置获得的核酸分子可以用于包括核酸测序在内的各种下游过程。
-
3.发明授权
公开(公告)号：EP2906715B1
公开(公告)日：2017-07-26
申请号：EP13783775.3
申请日：2013-10-15
Applicant: Life Technologies Corporation
Inventor： ANDERSEN, Mark , ROMAN, Steven
CPC classification number: C12Q1/6806 , C07H21/00 , C12Q1/6853 , C12Q2525/301 , C12Q2527/107 , C12Q2563/131 , C12Q2563/149
Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating one or more target nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the amount of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture using a primer having a first primer sequence and a second primer sequence that are complementary at a first melting temperature and are not complementary at a second melting temperature. In some aspects, target nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and template library preparation.
Abstract translation: 本发明提供了用于从样品中分离核酸分子的方法，组合物，试剂盒，系统和设备。 特别地，所述方法通常涉及标准化来自样品的靶核酸分子的浓度。 一方面，本发明涉及从引物延伸反应混合物中纯化引物延伸产物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的核酸分子可以用于各种下游过程，包括核酸测序。
-
4.发明公开COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT 审中-公开目标核酸富集的组合物，方法，系统和试剂盒
公开(公告)号：EP3252174A1
公开(公告)日：2017-12-06
申请号：EP17182486.5
申请日：2013-10-15
Applicant: Life Technologies Corporation
Inventor： ANDERSEN, Mark , ROMAN, Steven
CPC classification number: C12Q1/6806 , C07H21/00 , C12Q1/6853 , C12Q2525/301 , C12Q2527/107 , C12Q2563/131 , C12Q2563/149
Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating one or more target nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the amount of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture using a primer having a first primer sequence and a second primer sequence that are complementary at a first melting temperature and are not complementary at a second melting temperature. In some aspects, target nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and template library preparation.
Abstract translation: 本发明提供了用于从样品中分离一种或多种靶核酸分子的方法，组合物，试剂盒，系统和设备。 特别地，所述方法通常涉及标准化来自样品的靶核酸分子的量。 在一个方面，本发明涉及使用具有第一引物序列和第二引物序列的引物从引物延伸反应混合物中纯化引物延伸产物，所述引物在第一解链温度下互补并且在第二解链温度下不互补。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的靶核酸分子可用于各种下游过程，包括核酸测序和模板文库制备。
-
-
-
Search Results
4
records
(took 0.025 seconds)
