中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

39 records (took 0.026 seconds)

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    多重PCR的方法和组合

    公开(公告)号：WO2013081755A1

    公开(公告)日：2013-06-06

    申请号：PCT/US2012/062494

    申请日：2012-10-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6858 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. In yet another aspect, the invention relates to determining copy number variation with respect to gene or chromosome representation of a nucleic acid in the sample. In some aspects, the method for determining copy number variation of different target nucleic acids in a sample using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including diagnosis, predictive therapeutic regimes or other therapeutic purposes.

    Abstract translation: 本发明提供了可用于确定样品中存在的一种或多种核酸的拷贝数变异的方法，组合物，试剂盒，系统和装置。 在一些方面，该方法包括允许选择性扩增样品中一种或多种靶核酸的各种靶特异性引物。 在另一方面，本发明涉及确定相对于样品中核酸的基因或染色体表示的拷贝数变异。 在一些方面，使用所公开的方法，试剂盒，系统和装置确定样品中不同靶核酸的拷贝数变异的方法可以用于包括诊断，预测性治疗方案或其他治疗目的的各种下游过程。

    FLAT-FIELD IMAGING SYSTEM AND METHODS OF USE
    3.
    发明申请
    FLAT-FIELD IMAGING SYSTEM AND METHODS OF USE 审中-公开
    平面成像系统及其使用方法

    公开(公告)号：WO2012174142A1

    公开(公告)日：2012-12-20

    申请号：PCT/US2012/042290

    申请日：2012-06-13

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  ANDERSEN, Mark ,  PALLAS, Michael ,  WANG, Haopeng

    Inventor： ANDERSEN, Mark ,  PALLAS, Michael ,  WANG, Haopeng

    IPC: C12Q1/68

    CPC classification number: G01N15/04 ,  C12Q1/6816 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/629

    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

    Abstract translation: 提供了一种对齐多个目标的方法。 该方法包括生成多个目标。 第三阶段包括多个目标。 该方法还包括组合体积中的第一阶段，第二阶段和第三阶段。 第一相，第二相和第三相基本上是不混溶的，第三相与第一相和第二相流体连通，第一相，第二相和第三相可操作为 在体积中的第一相和第二相之间的第三相的配置。

    COMPOSITIONS AND METHODS FOR REUSING ARRAYS
    4.
    发明申请
    COMPOSITIONS AND METHODS FOR REUSING ARRAYS 审中-公开
    用于重新排列阵列的组合物和方法

    公开(公告)号：WO2009121028A1

    公开(公告)日：2009-10-01

    申请号：PCT/US2009/038681

    申请日：2009-03-27

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  ANDERSEN, Mark ,  ZHANG, Hua

    Inventor： ANDERSEN, Mark ,  ZHANG, Hua

    IPC: C12Q1/68

    CPC classification number: C12Q1/6837 ,  C12Q2521/301 ,  C12Q2521/531

    Abstract: The present disclosure relates generally to compositions and methods for the reuse of arrays, including microarrays. Specifically, the present disclosure discloses polynucleotide targets comprising nucleotide analogs that are not present within the probe polynucleotides immobilized on the array. The nucleotide-analog containing targets can be chemically modified to reduce their thermal stability and thus easier to remove from the array. In preferred embodiments, the disclosure relates to DNA probes hybridized to single-stranded deoxyribouridine-containing targets, the targets subsequently being chemically modified using a uracil DNA glycosylase and/or nuclease. Accordingly, the disclosure allows for the glycosylase treated, deoxyuridine-containing targets to be removed from the array by exposure to less stringent denaturing conditions than otherwise would have been required. Using less stringent denaturing conditions permits reuse of the array by reducing damage to the probe polynucleotides immobilized on the array during target removal.

    Abstract translation: 本公开一般涉及用于重新使用阵列（包括微阵列）的组合物和方法。 具体地，本公开公开了包含不存在于固定在阵列上的探针多核苷酸内的核苷酸类似物的多核苷酸靶。 可以化学修饰含核苷酸类似物的靶标，以降低其热稳定性，从而更容易从阵列中除去。 在优选的实施方案中，本公开涉及与单链脱氧核糖嘌呤的靶物杂交的DNA探针，随后使用尿嘧啶DNA糖基化酶和/或核酸酶化学修饰靶标。 因此，本公开允许经过糖基化酶处理的含脱氧尿苷的靶通过暴露于不同于本来需要的较不严格的变性条件下从阵列中除去。 使用较不严格的变性条件允许通过在目标去除期间减少固定在阵列上的探针多核苷酸的损伤来重新使用阵列。

    COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT
    6.
    发明申请
    COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT 审中-公开
    靶核酸成分的组成，方法，系统和工具

    公开(公告)号：WO2014062717A1

    公开(公告)日：2014-04-24

    申请号：PCT/US2013/065108

    申请日：2013-10-15

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ANDERSEN, Mark ,  ROMAN, Steven

    IPC: C12Q1/68 ,  C12N15/10

    CPC classification number: C12Q1/6806 ,  C07H21/00 ,  C12Q1/6853 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2563/131 ,  C12Q2563/149

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating one or more target nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the amount of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture using a primer having a first primer sequence and a second primer sequence that are complementary at a first melting temperature and are not complementary at a second melting temperature. In some aspects, target nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and template library preparation.

    Abstract translation: 本发明提供了可用于从样品中分离一种或多种靶核酸分子的方法，组合物，试剂盒，系统和装置。 特别地，所述方法通常涉及从样品归一化靶核酸分子的量。 一方面，本发明涉及使用具有第一引物序列和第二引物序列的引物从引物延伸反应混合物纯化引物延伸产物，所述第一引物序列和第二引物序列在第一熔融温度下互补且在第二熔融温度下不互补。 在一些方面，使用公开的方法，试剂盒，系统和装置获得的靶核酸分子可以用于包括核酸测序和模板文库制备在内的各种下游过程。

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    7.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：WO2012149438A9

    公开(公告)日：2012-11-01

    申请号：PCT/US2012/035612

    申请日：2012-04-27

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    8.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    用于多重PCR的方法和组合物

    公开(公告)号：WO2013081864A1

    公开(公告)日：2013-06-06

    申请号：PCT/US2012/065650

    申请日：2012-11-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    Abstract translation: 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 具体而言，提供了允许选择性扩增一个或多个靶序列的各种靶特异性引物。 一方面，本发明涉及用于选择性扩增与癌症或遗传疾病相关的一个或多个靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增的靶序列可用于各种下游过程，包括核酸测序并用于检测遗传变体的存在。

    COMPOSITIONS AND METHODS FOR HIGHLY SENSITIVE DETECTION OF TARGET SEQUENCES IN MULTIPLEX REACTIONS
    9.
    发明申请
    COMPOSITIONS AND METHODS FOR HIGHLY SENSITIVE DETECTION OF TARGET SEQUENCES IN MULTIPLEX REACTIONS 审中-公开

    公开(公告)号：WO2022140793A1

    公开(公告)日：2022-06-30

    申请号：PCT/US2021/073095

    申请日：2021-12-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ALLEN, Michael ,  ANDERSEN, Mark

    IPC: C12Q1/6858 ,  C12Q1/6855

    Abstract: Efficient methods for production of targeted libraries from complex samples is desirable for a variety of nucleic acid analyses. Provided are methods of selectively blocking abundant targets present in a sample for preparing libraries of target nucleic acid sequences, thereby allowing for rapid production of highly multiplexed targeted libraries and analysis of low frequency sequences, including sequencing applications. Methods optionally include use of unique tag sequences. Methods comprise contacting a nucleic acid sample with a plurality of target specific primers or adapters capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; ligating the digested target amplicons or repairing the digested target amplicons; and amplifying the ligated or repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the reactions further comprise target specific primers that are not capable of completely processing the workflow, resulting in non-useful amplicon production and thereby blocking selected target sequences, e.g., those present in high abundance in the sample. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences, which are optimized for detection of low frequency target sequences.

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    10.
    发明申请
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 审中-公开

    公开(公告)号：WO2019006392A1

    公开(公告)日：2019-01-03

    申请号：PCT/US2018/040432

    申请日：2018-06-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： ANDERSEN, Mark ,  MAZUR, Daniel

    IPC: C12Q1/6806 ,  C12Q1/6853

    Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

Patent Agency Ranking