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    DETECTION OF IMMOBILIZED NUCLEIC ACID
    3.
    发明申请
    DETECTION OF IMMOBILIZED NUCLEIC ACID 审中-公开
    检测固定化核酸

    公开(公告)号：US20150247799A1

    公开(公告)日：2015-09-03

    申请号：US14636063

    申请日：2015-03-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Matthew BEAUDET ,  William COX ,  Stephen YUE

    IPC: G01N21/64 ,  G01N1/30 ,  C09B23/04

    CPC classification number: G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

    Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.

    Abstract translation: 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。

    Methine-Substituted Cyanine Dye Compounds
    4.
    发明申请
    Methine-Substituted Cyanine Dye Compounds 有权
    甲基取代的Cyanine染料化合物

    公开(公告)号：US20150191600A1

    公开(公告)日：2015-07-09

    申请号：US14662397

    申请日：2015-03-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jason DALLWIG ,  David HAGEN ,  Ching-Ying CHEUNG ,  Gerald THOMAS ,  Stephen YUE

    IPC: C09B23/10 ,  C09B23/06 ,  C12Q1/68 ,  C09B23/04

    CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

    Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

    Abstract translation: 具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

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