公开(公告)号：WO2017007774A1

公开(公告)日：2017-01-12

申请号：PCT/US2016/041012

申请日：2016-07-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2525/197 ,  C12Q2565/549 ,  C12Q2563/149

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

Abstract translation: 水凝胶网络包括具有偶联位点的水凝胶聚合物，在末端与偶联位点处的水凝胶聚合物共轭的寡核苷酸和耦合在寡核苷酸的末端与偶联位点之间的功能部分。 这样的水凝胶网络可以通过以下方法形成：包括活化底物的偶联位点并将与寡核苷酸的末端连接的连接体结合到活化的偶联位点，连接在寡核苷酸的末端和 接头部分。

公开(公告)号：EP3320115B1

公开(公告)日：2020-09-02

申请号：EP16742101.5

申请日：2016-07-05

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

IPC: C12Q1/6834 ,  C12Q1/6874

公开(公告)号：EP3320115A1

公开(公告)日：2018-05-16

申请号：EP16742101.5

申请日：2016-07-05

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

IPC: C12Q1/68

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.