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    SUBSTRATES AND METHODS USEFUL IN SEQUENCING
    1.
    发明申请
    SUBSTRATES AND METHODS USEFUL IN SEQUENCING 审中-公开
    基板和方法有用的顺序

    公开(公告)号：WO2017007774A1

    公开(公告)日：2017-01-12

    申请号：PCT/US2016/041012

    申请日：2016-07-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874 ,  C12Q2565/518 ,  C12Q2525/117 ,  C12Q2525/197 ,  C12Q2565/549 ,  C12Q2563/149

    Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

    Abstract translation: 水凝胶网络包括具有偶联位点的水凝胶聚合物，在末端与偶联位点处的水凝胶聚合物共轭的寡核苷酸和耦合在寡核苷酸的末端与偶联位点之间的功能部分。 这样的水凝胶网络可以通过以下方法形成：包括活化底物的偶联位点并将与寡核苷酸的末端连接的连接体结合到活化的偶联位点，连接在寡核苷酸的末端和 接头部分。

    CONJUGATION OF CARBOXYL FUNCTIONAL HYDROPHILIC BEADS
    2.
    发明申请
    CONJUGATION OF CARBOXYL FUNCTIONAL HYDROPHILIC BEADS 审中-公开
    羧酸官能水凝胶珠的结合

    公开(公告)号：WO2017004559A1

    公开(公告)日：2017-01-05

    申请号：PCT/US2016/040767

    申请日：2016-07-01

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

    Inventor： FONNUM, Geir ,  MENCHEN, Steven M. ,  LUI, Alfred ,  XIANG, Guobing ,  LARSEN, Synne

    IPC: C12Q1/68 ,  G01N33/543

    CPC classification number: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629

    Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

    Abstract translation: 聚合物基质，例如聚合物涂层或聚合物水凝胶网络，包括羧基部分，其可以用作受体或分析物分子可以连接到的缀合位点。 在一个实例中，聚合物底物包括具有链烷酸部分或其衍生物的聚丙烯酰胺聚合物网络，其可与羧基活化化合物反应，以在聚丙烯酰胺网络上提供活化的链烷酸酯部分。 胺封端的核酸可以与活化的链烷酸酯部分反应，以通过烷基酰胺部分将核酸捕获到聚合物网络。

    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME
    4.
    发明申请
    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME 审中-公开
    聚合聚合物颗粒及其制备方法

    公开(公告)号：WO2013119956A1

    公开(公告)日：2013-08-15

    申请号：PCT/US2013/025352

    申请日：2013-02-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

    Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

    Abstract translation: 缀合底物的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在存在下将生物分子复合物偶联到亚基 的非水溶剂。 底物或固体支持物是聚合物颗粒，待沉淀在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME
    9.
    发明公开
    CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME 审中-公开
    共轭聚合物颗粒及其制备方法

    公开(公告)号：EP3228715A2

    公开(公告)日：2017-10-11

    申请号：EP17160771.6

    申请日：2013-02-08

    Applicant: Life Technologies Corporation

    Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

    Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

    Abstract translation: 缀合的衬底的方法包括：交换与亲脂性抗衡离子的生物分子相关的抗衡离子，以形成生物分子复合物，在非水溶剂中的生物分子复合物分散，并在非水溶剂的存在下偶联，所述生物分子复合物到基底上 。 底物或固体支持物是聚合物颗粒，要固定在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

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