公开(公告)号：NO844848L

公开(公告)日：1985-06-13

申请号：NO844848

申请日：1984-12-04

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER ,  CARRICO ROBERT J

IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/53 ,  G01N

公开(公告)号：NO844846L

公开(公告)日：1985-06-13

申请号：NO844846

申请日：1984-12-04

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER ,  CARRICO ROBERT J

IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/53 ,  G01N

公开(公告)号：AU560781B2

公开(公告)日：1987-04-16

申请号：AU5000185

申请日：1985-11-18

Applicant: MILES LAB

Inventor： BOGUSLAWSKI SOPHIE J ,  ANDERSON LESLIE H DERIEMER

IPC: G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53

Abstract: The rate of hybridization between two complementary polynucleotide segments in an aqueous medium is increased by the presence of the anionic polymers polyacrylate and polymethacrylate. The acceleration effect is particularly useful in nucleic acid hybridization assays involving immobilization of sample nucleic acids and the use of labeled probes. Nonspecific binding of probe to nitrocellulose supports is substantially lower in the presence of the present polymers than in the presence of the prior art accelerator dextran sulfate. Polyacrylate is particularly advantageous since it has been found to be effective at low concentrations and is significantly less expensive than the prior art compound.

公开(公告)号：AU3656084A

公开(公告)日：1985-06-20

申请号：AU3656084

申请日：1984-12-12

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER

IPC: C07K16/18 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/54

公开(公告)号：NO844848A

公开(公告)日：1985-06-13

申请号：NO844848

申请日：1984-12-04

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER ,  CARRICO ROBERT J

IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/53 ,  G01N

公开(公告)号：NO844847A

公开(公告)日：1985-06-13

申请号：NO844847

申请日：1984-12-04

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER

IPC: C07K16/18 ,  C12Q1/68 ,  C12Q1/70 ,  G01N

CPC classification number: C12Q1/6816 ,  C07K16/18 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/705 ,  C12Q2563/173 ,  C12Q2563/131

公开(公告)号：NO844846A

公开(公告)日：1985-06-13

申请号：NO844846

申请日：1984-12-04

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER ,  CARRICO ROBERT J

IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/53 ,  G01N

公开(公告)号：AU578436B2

公开(公告)日：1988-10-27

申请号：AU3656084

申请日：1984-12-12

Applicant: MILES LAB

Inventor： ALBARELLA JAMES PAUL ,  ANDERSON LESLIE H DERIEMER

IPC: C07K16/18 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53

公开(公告)号：AU5000185A

公开(公告)日：1986-05-29

申请号：AU5000185

申请日：1985-11-18

Applicant: MILES LAB

Inventor： BOGUSLAWSKI SOPHIE J ,  ANDERSON LESLIE H DERIEMER

IPC: G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53

Abstract: The rate of hybridization between two complementary polynucleotide segments in an aqueous medium is increased by the presence of the anionic polymers polyacrylate and polymethacrylate. The acceleration effect is particularly useful in nucleic acid hybridization assays involving immobilization of sample nucleic acids and the use of labeled probes. Nonspecific binding of probe to nitrocellulose supports is substantially lower in the presence of the present polymers than in the presence of the prior art accelerator dextran sulfate. Polyacrylate is particularly advantageous since it has been found to be effective at low concentrations and is significantly less expensive than the prior art compound.

公开(公告)号：NO844849L

公开(公告)日：1985-06-13

申请号：NO844849

申请日：1984-12-04

Applicant: MILES LAB

Inventor： ALBARELLA JAMES P ,  ANDERSON LESLIE H DERIEMER ,  CARRICO ROBERT J

IPC: C12Q1/68 ,  G01N20060101 ,  G01N33/52 ,  G01N33/53 ,  G01N