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    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME-CLEAVABLE SUBSTRATE AS LABEL
    2.
    发明专利
    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME-CLEAVABLE SUBSTRATE AS LABEL 未知

    公开(公告)号：CA1148080A

    公开(公告)日：1983-06-14

    申请号：CA396228

    申请日：1982-02-12

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  BURD JOHN F

    IPC: C12Q1/34 ,  G01N33/54

    Abstract: An improved homogeneous specific binding assay method for determining a ligand in a liquid medium, wherein a reaction mixture is formed by combining the liquid medium with reagent means, including a conjugate having a label component and a binding component, to form a binding reaction system having a bound-species and a free-species of the labeled conjugate, the label component of the conjugate comprising an enzyme substrateactive portion and an indicator portion whereby the conjugate is cleavable by an enzyme to produce a detectable indicator product, the labeled conjugate being substantially inactive as a substrate for the enzyme when in the bound-species, and wherein the cleaving enzyme is added to the reaction mixture and the resulting indicator product measured as a function of the amount of the ligand in said liquid medium. The improvement comprises employing as the label component of the conjugate, a residue having the formula: wherein R is a linking group through which the residue is covalently bound to the binding component, and employing .beta.-galactosidase as the cleaving enzyme whereby the .beta.-galactosyl group in the residue can be cleaved to release a detectable dye product.

    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME CLEAVABLE SUBSTRATE AS LABEL
    4.
    发明专利
    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME CLEAVABLE SUBSTRATE AS LABEL 未知

    公开(公告)号：CA1133474A

    公开(公告)日：1982-10-12

    申请号：CA320897

    申请日：1979-02-06

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  BURD JOHN F

    IPC: C07D209/48 ,  C07D233/78 ,  C07H17/075 ,  C07H21/00 ,  G01N33/533 ,  G01N33/535 ,  C07H17/06

    Abstract: An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula: G-D-R wherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens such as drugs, particularly the aminoglycoside antibiotics.

    HOMOGENEOUS IMMUNOASSAY WITH LABELED MONOCLONAL ANTI- ANALYTE
    6.
    发明专利
    HOMOGENEOUS IMMUNOASSAY WITH LABELED MONOCLONAL ANTI- ANALYTE 未知

    公开(公告)号：CA1186622A

    公开(公告)日：1985-05-07

    申请号：CA415983

    申请日：1982-11-19

    Applicant: MILES LAB

    Inventor： BURD JOHN F

    IPC: G01N33/577 ,  C07K16/18 ,  G01N33/542 ,  G01N33/58 ,  G01N33/54 ,  C12Q1/00

    Abstract: A homogeneous immunoassay method for determining an analyte, particularly analytes of molecular weight greater than about 10,000 (e.g., proteins), wherein sample is combined with a labeled monoclonal anti-analyte preparation in which the anti-analyte is substantially the only labeled component. Binding of the analyte with labeled anti-analyte results directly in a change in the detectable response generated by the label, usually due to steric constraints. The method provides a simple homogeneous technique which does not require the preparation of purified analyte in order to prepare assay reagents. Alternatively, the sample is combined with the labeled monoclonal anti-analyte and with a macromolecular conjugate having analyte epitope sites and binding of the labeled anti-analyte to such conjugate results in a change in the response of the label.

    REAGENTS FOR USE IN BINDING ASSAYS TO DETERMINE VALPROIC ACID
    7.
    发明专利
    REAGENTS FOR USE IN BINDING ASSAYS TO DETERMINE VALPROIC ACID 未知

    公开(公告)号：CA1157017A

    公开(公告)日：1983-11-15

    申请号：CA416114

    申请日：1982-11-22

    Applicant: MILES LAB

    Inventor： BUCKLER ROBERT T ,  BURD JOHN F ,  WONG RAPHAEL C

    IPC: C07H7/06

    Abstract: Reagents for use in binding assays, particularly immunoassays, to determine valproic acid in liquid media such as serum. Such reagents include antibody to valproic acid and .beta.-galactosyl-umbeiliferone-valproic acid conjugates. Also provided are compounds used to prepare such reagents, including valproic acid immunogen conju gates and intermediates in the synthesis of such immunogen conjugates and .beta.-galactosyl-umbelliferone-labeled conjugates.

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