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    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME-CLEAVABLE SUBSTRATE AS LABEL
    3.
    发明专利
    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME-CLEAVABLE SUBSTRATE AS LABEL 未知

    公开(公告)号：CA1148080A

    公开(公告)日：1983-06-14

    申请号：CA396228

    申请日：1982-02-12

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  BURD JOHN F

    IPC: C12Q1/34 ,  G01N33/54

    Abstract: An improved homogeneous specific binding assay method for determining a ligand in a liquid medium, wherein a reaction mixture is formed by combining the liquid medium with reagent means, including a conjugate having a label component and a binding component, to form a binding reaction system having a bound-species and a free-species of the labeled conjugate, the label component of the conjugate comprising an enzyme substrateactive portion and an indicator portion whereby the conjugate is cleavable by an enzyme to produce a detectable indicator product, the labeled conjugate being substantially inactive as a substrate for the enzyme when in the bound-species, and wherein the cleaving enzyme is added to the reaction mixture and the resulting indicator product measured as a function of the amount of the ligand in said liquid medium. The improvement comprises employing as the label component of the conjugate, a residue having the formula: wherein R is a linking group through which the residue is covalently bound to the binding component, and employing .beta.-galactosidase as the cleaving enzyme whereby the .beta.-galactosyl group in the residue can be cleaved to release a detectable dye product.

    HOMOGENOUS SPECIFIC BINDING ASSAY METHOD AND COMPOSITION AND DEVICE FOR USE THEREIN
    4.
    发明专利
    HOMOGENOUS SPECIFIC BINDING ASSAY METHOD AND COMPOSITION AND DEVICE FOR USE THEREIN 未知

    公开(公告)号：CA1082577A

    公开(公告)日：1980-07-29

    申请号：CA249745

    申请日：1976-04-07

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

    IPC: G01N33/542 ,  C07D209/48 ,  C07D237/32 ,  C07D493/10 ,  C07D495/04 ,  C07H19/20 ,  C07H19/207 ,  C07H21/00 ,  C12Q1/00 ,  G01N31/00 ,  G01N33/50 ,  G01N33/532 ,  G01N33/533 ,  G01N33/536 ,  G01N33/543 ,  G01N33/60 ,  G01N33/16

    Abstract: OE OF DISCLOSURE A test composition, device, and method for their use in a homogenous specific binding assay which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium The test composition and device comprise a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

    CHEMILUMINESCENT AMINOPHTHALHYDRAZIDE-LABELED CONJUGATES FOR USE IN SPECIFIC BINDING ASSAYS
    7.
    发明专利
    CHEMILUMINESCENT AMINOPHTHALHYDRAZIDE-LABELED CONJUGATES FOR USE IN SPECIFIC BINDING ASSAYS 未知

    公开(公告)号：CA1146937A

    公开(公告)日：1983-05-24

    申请号：CA327349

    申请日：1979-05-10

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J

    IPC: G01N33/532 ,  C07D209/48 ,  C07D237/32 ,  G01N33/533 ,  G01N33/58 ,  C07G7/00 ,  C07J43/00

    Abstract: Chemiluminescent-labeled conjugates of the formula: Chemi-R-L wherein Chemi represents a moiety which is capable of undergoing a change in chemical structure with the production of light, R is a linking group, and L is a specifically bindable ligand, such as an antigenic protein or polypeptide, a hapten or an antibody, or a binding analog thereof. The chemiluminescent moiety may be luminol, isoluminol, pyrogallol or luciferin. Preferred labeled conjugates are of the formula: wherein one of R1 and R2 is hydrogen and the other is -NR3R4; R3 is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R4 is wherein ? = 1-3 and L(CO? is the ligand or analog bound through an amide bond. Intermediates produces in the synthesis of such preferred conjugates are also disclosed.

    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME CLEAVABLE SUBSTRATE AS LABEL
    10.
    发明专利
    SPECIFIC BINDING ASSAY EMPLOYING AN ENZYME CLEAVABLE SUBSTRATE AS LABEL 未知

    公开(公告)号：CA1133474A

    公开(公告)日：1982-10-12

    申请号：CA320897

    申请日：1979-02-06

    Applicant: MILES LAB

    Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  BURD JOHN F

    IPC: C07D209/48 ,  C07D233/78 ,  C07H17/075 ,  C07H21/00 ,  G01N33/533 ,  G01N33/535 ,  C07H17/06

    Abstract: An improved specific binding assay method and reagent for determining a ligand in a liquid medium employing, as an enzyme-cleavable substrate label, a residue having the formula: G-D-R wherein G is a glycone, D is a dye indicator moiety, and R is a linking group through which the label residue is covalently bound to a binding component of a conventional binding assay system, such as the ligand, an analog thereof, or a specific binding partner thereof. The monitored characteristic of the label is the release of a detectable product, usually a fluorogen or chromogen, upon enzymatic cleavage of the glycosidic linkage between the glycone and the dye indicator moiety. The assay method may follow a homogeneous or heterogeneous format. The preferred glycone is a .beta.-galactosyl group and the preferred dye indicator moiety is an umbelliferone residue. The improved assay is particularly suited to the determination of haptens such as drugs, particularly the aminoglycoside antibiotics.

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