公开(公告)号：CA1079632A

公开(公告)日：1980-06-17

申请号：CA288598

申请日：1977-10-13

Applicant: MILES LAB

Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

IPC: G01N33/536 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532 ,  G01N33/542 ,  G01N33/543 ,  G01N33/16 ,  G01N31/14

Abstract: A specific binding assay method employing, as a labeling substance, a reversibly binding enzyme modulator for the detection of a ligand in a liquid medium. The method follows conventional specific binding assay techniques of either the homogeneous or heterogeneous type wherein the liquid medium to be assayed is combined with reagent means that includes a labeled conjugate to form a binding reaction system having a bound-species and a free-species of the conjugate. The amount of conjugate resulting in the bound-species or the free-species is a function of the amount of ligand present in the liquid medium assayed. In the present invention, the labeled conjugate comprises a reversibly binding enzyme modulator covalently linked to a binding component of the binding reaction system. The distribution of the conjugate between the bound-species and the free-species is determined by addition of an enzyme whose modulator and measuring the resulting activity of the enzyme. The enzyme modulator may be a conventional enzyme inhibitor, preferably of the competitive type, or an allosteric effector.

公开(公告)号：CA1082577A

公开(公告)日：1980-07-29

申请号：CA249745

申请日：1976-04-07

Applicant: MILES LAB

Inventor： BOGUSLASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

IPC: G01N33/542 ,  C07D209/48 ,  C07D237/32 ,  C07D493/10 ,  C07D495/04 ,  C07H19/20 ,  C07H19/207 ,  C07H21/00 ,  C12Q1/00 ,  G01N31/00 ,  G01N33/50 ,  G01N33/532 ,  G01N33/533 ,  G01N33/536 ,  G01N33/543 ,  G01N33/60 ,  G01N33/16

Abstract: OE OF DISCLOSURE A test composition, device, and method for their use in a homogenous specific binding assay which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium The test composition and device comprise a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

公开(公告)号：CA1078712A

公开(公告)日：1980-06-03

申请号：CA249744

申请日：1976-04-07

Applicant: MILES LAB

Inventor： BOGUSIASKI ROBERT C ,  CARRICO ROBERT J ,  CHRISTNER JAMES E

IPC: G01N33/542 ,  C07D209/48 ,  C07D237/32 ,  C07D493/10 ,  C07D495/04 ,  C07H19/20 ,  C07H19/207 ,  C07H21/00 ,  C12Q1/00 ,  G01N31/00 ,  G01N33/50 ,  G01N33/532 ,  G01N33/533 ,  G01N33/536 ,  G01N33/543 ,  G01N33/60 ,  G01N33/16

Abstract: An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques. After the necessary separation of the boundand free-phases resulting in the specific binding reaction system, the extent of binding of the labeled constituent is determined by contacting either phase with the necessary materials to form the predetermined monitoring reaction system in which the labeling substance is active and assessing reactant activity therein.

公开(公告)号：CA956097A

公开(公告)日：1974-10-15

申请号：CA108577

申请日：1971-03-24

Applicant: MILES LAB

Inventor： FADER MARSHALL L ,  CHRISTNER JAMES E

IPC: G01N33/487 ,  G01N33/78

Abstract: The thyroid hormone content of blood serum can be readily determined by using an in vitro radioactive thyroid function test system containing a "protein error" pH indicator such as bromcresol purple. Such a "protein error" indicator provides color and detects the presence of the test sample, inhibits the formation of free iodide and decreases the adsorption of radioactive tagged thyroid substance to the walls of the container enclosing same.