公开(公告)号：AU3759185A

公开(公告)日：1985-10-03

申请号：AU3759185

申请日：1985-01-10

Applicant: MILES LAB

Inventor： BUCKLER ROBERT T ,  HATCH ROBERT P

IPC: C07D215/22 ,  C07D215/20 ,  C07D215/38 ,  C07D215/54 ,  C07D311/20 ,  C07D407/12 ,  C07D471/16 ,  C07D491/06 ,  C07D491/16 ,  C07F9/60 ,  C12Q1/28 ,  G01N21/76 ,  G01N21/78 ,  G01N33/50 ,  G01N33/52

公开(公告)号：CA1288708C

公开(公告)日：1991-09-10

申请号：CA510924

申请日：1986-06-05

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  HATCH ROBERT P ,  PATTERSON WILLIAM L

IPC: G01N33/53 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/545

Abstract: Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample. MS-1419

公开(公告)号：AU553438B2

公开(公告)日：1986-07-17

申请号：AU3759185

申请日：1985-01-10

Applicant: MILES LAB

Inventor： BUCKLER ROBERT T ,  HATCH ROBERT P

IPC: C07D215/22 ,  C07D215/20 ,  C07D215/38 ,  C07D215/54 ,  C07D311/20 ,  C07D407/12 ,  C07D471/16 ,  C07D491/06 ,  C07D491/16 ,  C07F9/60 ,  C12Q1/28 ,  G01N21/76 ,  G01N21/78 ,  G01N33/50 ,  G01N33/52

公开(公告)号：CA1265424A

公开(公告)日：1990-02-06

申请号：CA470844

申请日：1984-12-21

Applicant: MILES LAB

Inventor： BUCKLER ROBERT T ,  HATCH ROBERT P

IPC: C07D215/22 ,  C07D215/20 ,  C07D215/38 ,  C07D215/54 ,  C07D311/20 ,  C07D407/12 ,  C07D471/16 ,  C07D491/06 ,  C07D491/16 ,  C07F9/60 ,  C12Q1/28 ,  G01N21/76 ,  G01N21/78 ,  G01N33/50 ,  G01N33/52

Abstract: Fluorogenic indicator compounds responsive to the presence of hydrogen peroxide and methods and test compositions for determining hydrogen peroxide in a test sample. Preferred indicator compounds are 3,4-dihydrocoumarin derivatives and 3,4-dihydro-2-quinolone derivatives, especially those having a dialkylamino group at the 7-position. Such particularly preferred compounds are of the formula: wherein R1 is hydrogen, cyano, or -COOR6, -CONHR6 or -CON(R6)2 where R6 is hydrogen, alkyl, alkenyl, or aryl; R3 and R4 are lower alkyl; and Y is =O or =N-R5 where R5 is hydrogen or lower alkyl. The indicator compounds yield highly fluorescent products upon oxidation by hydrogen peroxide in the presence of a peroxidatively active substance and are useful in analytical systems which generate hydrogen peroxide in response to an analyte under determination in a test sample.

公开(公告)号：DE3466015D1

公开(公告)日：1987-10-15

申请号：DE3466015

申请日：1984-03-02

Applicant: MILES LAB

Inventor： BUCKLER ROBERT T ,  HATCH ROBERT P

IPC: C07D215/38 ,  C07C67/00 ,  C07C227/00 ,  C07C229/44 ,  C07D215/36 ,  C07D215/54 ,  C07D311/16 ,  C07D311/20 ,  C12Q1/28 ,  G01N31/00 ,  G01N31/22

公开(公告)号：CA1294285C

公开(公告)日：1992-01-14

申请号：CA446312

申请日：1984-01-30

Applicant: MILES LAB

Inventor： BUCKLER ROBERT T ,  HATCH ROBERT P

IPC: C07D215/38 ,  C07C67/00 ,  C07C227/00 ,  C07C229/44 ,  C07D215/36 ,  C07D215/54 ,  C07D311/16 ,  C07D311/20 ,  C12Q1/28 ,  G01N31/00 ,  G01N31/22 ,  C07D215/18

Abstract: Optical indicator chalcogen (selenide or sulfide) compounds responsive to oxidants, e.g., hydrogen peroxide, and methods for preparing and using such indicators. Upon oxidation the resulting intermediate undergoes spontaneous elimination of the chalcogen residue to yield a signal compound which provides an' optical signal such as fluorescence. Preferred fluorogenic indicator compounds are 3-chalcogen-3,4-dihydrocoumarin derivatives and 3-chalcogen-3,4-dihydro-2-quinolone derivatives. Such indicator compounds provide highly fluorescent products upon oxidation by hydrogen peroxide and are useful in analytical systems which generate hydrogen peroxide in response to the analyte under determination. ALK/mc MS-1275