公开(公告)号：CA1288709C

公开(公告)日：1991-09-10

申请号：CA511592

申请日：1986-06-13

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  PATTERSON WILLIAM L

IPC: G01N33/53 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/545

Abstract: Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon having amide groups that have been derivatized to amidine residues. Derivatization of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by reaction with an amine. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample. MS-1422

公开(公告)号：CA1288708C

公开(公告)日：1991-09-10

申请号：CA510924

申请日：1986-06-05

Applicant: MILES LAB

Inventor： CARRICO ROBERT J ,  HATCH ROBERT P ,  PATTERSON WILLIAM L

IPC: G01N33/53 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/545

Abstract: Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample. MS-1419