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公开(公告)号：US20160090588A1
公开(公告)日：2016-03-31
申请号：US14889374
申请日：2014-05-07
Applicant: MICRONICS, INC.
Inventor： Alan K. Lofquist , C. Frederick Battrell , Heather K. Bouzek
CPC classification number: C12N15/1017 , B01L3/502753 , B01L2300/0681 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0406 , B01L2400/0481 , B01L2400/049 , C12Q1/6806
Abstract: An integrated “lab-on-a-chip” microfluidic device performs nucleic acid sample preparation and diagnostic analysis from test samples containing cells and/or particles. The device analyzes DNA or RNA targets, or both, from a common test sample. Dried and/or liquid reagents necessary for nucleic acid sample preparation and analysis are contained on the device, such that the device only requires addition of test sample. Clay mineral and alkaline buffer reagents are employed for overcoming the problems of nucleic acid degradation and contamination during sample preparation. The device may include a composite filter to separate plasma or serum from other blood constituents when the test sample is a blood product. The microfluidic device utilizes a plurality of microfluidic channels, inlets, valves, membranes, pumps, and other elements arranged in various configurations to manipulate the flow of the liquid sample, in particular, in order to prepare nucleic acids and perform further diagnostic analysis.
Abstract translation: 集成的“芯片上实验室”微流体装置从含有细胞和/或颗粒的测试样品进行核酸样品制备和诊断分析。 该设备从普通测试样品中分析DNA或RNA靶标，或两者。 在设备上包含用于核酸样品制备和分析所需的干燥和/或液体试剂，使得该装置仅需要加入测试样品。 粘土矿物和碱性缓冲剂用于克服样品制备过程中核酸降解和污染的问题。 当测试样品是血液制品时，该装置可以包括复合过滤器以将血浆或血清与其它血液成分分离。 微流体装置利用多个微流体通道，入口，阀，膜，泵和以各种配置布置的其它元件来操纵液体样品的流动，特别是为了制备核酸并进行进一步的诊断分析。
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公开(公告)号：US10087440B2
公开(公告)日：2018-10-02
申请号：US14889374
申请日：2014-05-07
Applicant: Micronics, Inc.
Inventor： Alan K. Lofquist , C. Frederick Battrell , Heather K. Bouzek
IPC: C12N15/10 , B01L3/00 , C12Q1/6806
Abstract: An integrated “lab-on-a-chip” microfluidic device performs nucleic acid sample preparation and diagnostic analysis from test samples containing cells and/or particles. The device analyzes DNA or RNA targets, or both, from a common test sample. Dried and/or liquid reagents necessary for nucleic acid sample preparation and analysis are contained on the device, such that the device only requires addition of test sample. Clay mineral and alkaline buffer reagents are employed for overcoming the problems of nucleic acid degradation and contamination during sample preparation. The device may include a composite filter to separate plasma or serum from other blood constituents when the test sample is a blood product. The microfluidic device utilizes a plurality of microfluidic channels, inlets, valves, membranes, pumps, and other elements arranged in various configurations to manipulate the flow of the liquid sample, in particular, in order to prepare nucleic acids and perform further diagnostic analysis.
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公开(公告)号：US20180125464A1
公开(公告)日：2018-05-10
申请号：US15569701
申请日：2016-04-29
Applicant: Micronics, Inc.
Inventor： Andrew Kolb , Alan K. Lofquist , C. Frederick Battrell
CPC classification number: A61B10/0096 , A61B10/0045 , A61B10/02 , B01L3/50825 , B01L2200/026 , B01L2200/027 , B01L2200/16 , B01L2300/043 , B01L2300/044 , B01L2300/0681 , B01L2400/0683 , G01N1/00
Abstract: A specimen collection and delivery apparatus for collecting clinical specimens and delivery of samples thereof to, e.g., microfluidic testing devices for diagnostic analysis is disclosed. The specimen collection and delivery apparatus includes a closure housing for coupling with a sample tube at one end, an open luer for coupling with a complementary luer on a microfluidic device at the other end, and a filter matrix disposed in the interior. The filter matrix is air permeable and liquid impermeable at atmospheric pressure and prevents sample from flowing through the open luer end when coupled to a sample tube containing a specimen. Under vacuum, the liquid sample fraction of the specimen can be drawn through the filter matrix and into the microfluidic testing device through the luer connection for diagnostic analysis.
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4.发明申请公开(公告)号：US20170113221A1
公开(公告)日：2017-04-27
申请号：US15317365
申请日：2015-06-11
Applicant: Micronics, Inc.
Inventor： Danny Hoffman , Asa Bergdahl , Megan Couch , Raf Rivera , Matthew Scott Bragd , Alan K. Lofquist , Andrew Kolb , Justin L. Kay
CPC classification number: B01L3/502715 , B01L3/50273 , B01L3/52 , B01L3/527 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0684 , B01L2200/14 , B01L2200/16 , B01L2300/044 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/14 , B01L2400/0481 , B01L2400/0487 , B01L2400/0638 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N35/00069 , G01N2035/00158
Abstract: Disclosed is a microassay testing system, including a microfluidic cartridge and a compact microprocessor-controlled instrument for fluorometric assays in liquid samples, the cartridge having integrated process controls and positive and negative assay controls. The instrument has a scanning detector head incorporating multiple optical channels. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a process control. Integrated positive and negative assay controls provide enhanced assay validation capabilities and facilitate analysis of test results. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general.
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