公开(公告)号：WO03095986A8

公开(公告)日：2005-05-06

申请号：PCT/IB0301821

申请日：2003-05-09

Applicant: AMERSHAM BIOSCIENCES NIAGARA I ,  RAMM PETER ,  ALEXAMDROV YURIY ,  CYBUCH JERRY ,  DONDERS PAUL ,  ZARATE CARLOS ,  SOLTYS BOHDAN J

Inventor： RAMM PETER ,  ALEXAMDROV YURIY ,  CYBUCH JERRY ,  DONDERS PAUL ,  ZARATE CARLOS ,  SOLTYS BOHDAN J

IPC: G01N15/00 ,  G01N15/14 ,  G01N21/17 ,  G01N21/64 ,  G01N33/48 ,  G06T1/00 ,  G01N33/50 ,  G02B21/24

CPC classification number: G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A system for performing automated cell screening in drug discovery includes an automated microscope (100), a fast autofocus device, and a digital imaging system (500). Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

Abstract translation: 用于在药物发现中进行自动细胞筛选的系统包括自动显微镜（100），快速自动聚焦装置和数字成像系统（500）。 过程在软件中实现，通过该软件，相关的细胞材料通过最小的用户交互被分段和量化。 以下领域的改进：图像处理的已知方法以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。

公开(公告)号：WO0165461A2

公开(公告)日：2001-09-07

申请号：PCT/IB0100297

申请日：2001-03-02

Applicant: IMAGING RES INC ,  RAMM PETER ,  NADON ROBERT ,  SHI PEIDE

Inventor： RAMM PETER ,  NADON ROBERT ,  SHI PEIDE

IPC: G01N33/53 ,  C12Q1/68 ,  G01N37/00 ,  G06F17/18 ,  G06F19/20 ,  G06F19/24 ,  G06Q50/00 ,  G06F19/00

CPC classification number: G06F19/20 ,  C12Q1/6837 ,  G06F19/24

Abstract: An analytical process is disclosed, for discriminating data acquired from samples with overlapping distributions, and for improving and assessing the statistical validity of hybridization signal in arrays of assays. The process includes method of convolving data into two or more discrete probability density functions representing signal and nonsignal, discrete fluors, or other convolved independent variables. The system uses the probability density functions to assign hybridization signals, objectively, to one of the modeled distributions. Subsequent processes assess variability inherent to the arrays, and use this assessed variation to establish reliability scores and confidence limits for complete hybridization arrays, and for discrete hybridization assays within arrays.

Abstract translation: 公开了一种分析方法，用于区分从具有重叠分布的样品获得的数据，以及用于改进和评估测定阵列中杂交信号的统计学效度。 该过程包括将数据卷积成表示信号和非信号离散荧光或其它卷积的独立变量的两个或更多个离散概率密度函数的方法。 系统使用概率密度函数将杂交信号客观地分配给一个建模分布。 随后的过程评估阵列固有的变异性，并使用该评估的变异来建立完整杂交阵列的可靠性评分和置信限，以及阵列内的离散杂交测定。

公开(公告)号：WO0078991A3

公开(公告)日：2001-08-09

申请号：PCT/IB0000810

申请日：2000-06-19

Applicant: IMAGING RES INC ,  RAMM PETER ,  NADON ROBERT ,  SHI PEIDE

Inventor： RAMM PETER ,  NADON ROBERT ,  SHI PEIDE

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/00 ,  G01N33/48 ,  G01N33/50 ,  G06F19/20 ,  G06F19/24

CPC classification number: G06F19/20 ,  G06F19/24

Abstract: A method for improving the reliability and/or accuracy of physical measurements obtained from array hybridization studies performed on an array having a large number of genomic samples uses a small number of replicates insufficient for making precise and valid statistical inferences. This is overcome by estimating an error in measurement of a sample by averaging errors obtained when measuring the large number of samples or a subset of the large number of samples. The estimated sample error is utilized as a standard for accepting or rejecting the measurement of the respective sample. The samples may be independent or dependant in that correlated across two or more conditions.

Abstract translation: 通过在具有大量基因组样品的阵列上进行的阵列杂交研究获得的用于提高物理测量的可靠性和/或准确性的方法使用少量的重复，不足以进行精确和有效的统计推断。 通过在测量大量样本或大量样本的子集时获得的平均误差来估计样本的测量误差来克服这一点。 估计的样本误差被用作接受或拒绝相应样本的测量的标准。 样本可以是独立的或依赖于两个或多个条件相关的。

公开(公告)号：WO02068942A3

公开(公告)日：2003-01-03

申请号：PCT/IB0200590

申请日：2002-02-27

Applicant: IMAGING RES INC ,  ZARATE CARLOS ,  DONDERS PAUL ,  YEKTA AHMED ,  MASOUMI ZAHRA ,  RAMM PETER

Inventor： ZARATE CARLOS ,  DONDERS PAUL ,  YEKTA AHMED ,  MASOUMI ZAHRA ,  RAMM PETER

IPC: G01N21/64 ,  G01N21/78 ,  G01N33/483

CPC classification number: G01N21/6408 ,  G01N2201/0648

Abstract: The present invention relates to an apparatus and method for imaging time resolved fluorescence in biochemical and medical samples. In a primary aspect, the device includes a lens of large aperture, a flash lamp in the illumination path, a fast-acting solid state shutter or a gated detector in the emission path, a device for delivering homogenous monochromatic illumination to a plurality of wells distributed within a microwell plate, a digital camera of high quantum efficiency, and a computer. under computer control, the lamp is pulsed at short intervals. The fast-acting emission shutter or gated detector operates to limit exposure of the camera to a period some microseconds after the extinction of each lamp pulse, during which only delayed fluorescence is transmitted to the camera. The invention achieves simultaneous time resolved imaging of a plurality of samples, with high sensitivity and high throughput.

Abstract translation: 本发明涉及用于在生物化学和医学样品中成像时间分辨荧光的装置和方法。 在主要方面，该装置包括大孔径的透镜，照明路径中的闪光灯，发射路径中的快速固态快门或门控检测器，用于将均匀单色照明传送到多个孔的装置 分布在微孔板，高量子效率的数码相机和计算机中。 在计算机控制下，灯以短时间脉冲。 快速发射快门或门控检测器操作以将相机的曝光限制在每个灯脉冲消失之后几微秒的时段，在此期间仅将延迟的荧光传输到相机。 本发明实现多个样品的同时时间分辨成像，具有高灵敏度和高产量。

公开(公告)号：WO0103189A8

公开(公告)日：2001-05-17

申请号：PCT/EP0006132

申请日：2000-06-30

Applicant: FRAUNHOFER GES FORSCHUNG ,  LANDESBERGER CHRISTOF ,  REICHL HERBERT ,  ANSORGE FRANK ,  RAMM PETER ,  EHRMANN OSWIN

Inventor： LANDESBERGER CHRISTOF ,  REICHL HERBERT ,  ANSORGE FRANK ,  RAMM PETER ,  EHRMANN OSWIN

IPC: H01L25/18 ,  H01L21/301 ,  H01L21/78 ,  H01L21/98 ,  H01L23/52 ,  H01L25/065 ,  H01L25/07

CPC classification number: H01L21/6836 ,  H01L21/78 ,  H01L24/24 ,  H01L24/82 ,  H01L25/0652 ,  H01L25/0657 ,  H01L25/50 ,  H01L2221/68327 ,  H01L2221/6834 ,  H01L2224/24051 ,  H01L2224/24226 ,  H01L2224/32225 ,  H01L2224/73267 ,  H01L2225/0651 ,  H01L2225/06517 ,  H01L2225/06524 ,  H01L2225/06541 ,  H01L2225/06555 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01032 ,  H01L2924/01033 ,  H01L2924/01052 ,  H01L2924/01061 ,  H01L2924/01068 ,  H01L2924/01075 ,  H01L2924/014 ,  H01L2924/10329 ,  H01L2924/13055 ,  H01L2924/14 ,  H01L2924/30105 ,  H01L2924/351 ,  H01L2924/00

Abstract: The invention relates to a multichip module comprising a base chip (10), a top chip (16), whose thickness is less than 100 mu m, and an adhesive layer (22) which is placed between the base chip (10) and the top chip (16), and both chips are mechanically joined to one another. An electrically insulating planarization layer (24) is provided such that the top chip (16) is embedded in the planarization layer (24). A through hole (26a) in the planarization layer (24) is filled with an electrically conductive material, is connected, in an electrically conductive manner, to a connection surface of the base chip (10), and is connected, in an electrically conductive manner, to a connection surface (20) of the top chip (16) via a metallization (28, 30). Through holes (26a, 26b) in the planarization layer (24) enable the production of all connections between the chips, between the base chip or between the top chip and external contacts of the multichip module in a manner that is independent of the individual chips and while using conventional semiconductor processing steps.

Abstract translation: 一种多芯片模块包括基底芯片（10）和顶片（16）之间的基片（10），顶片（16），厚度小于100微米，和粘合层（22）， 两个芯片机械连接在一起。 电绝缘平面化层（24）被设置成使得在所述平坦化层（24）的顶片（16）嵌入。 在平坦化层的通孔（26a）的（24）中填充有导电材料并在一方面与基底芯片（10）的连接表面（20）和，另一方面，经由金属化（28,30）同一个连接表面（20） 顶片（16）导电连接。 通过通孔（26A，26B）在平坦化层（24），可以在基体之间的芯片之间的所有连接，管芯或顶片和使用传统的半导体处理步骤的多芯片模块的外部接触，独立于单个芯片之间 生产。

公开(公告)号：DD143581A1

公开(公告)日：1980-09-03

申请号：DD21298179

申请日：1979-05-18

Applicant: BEHLICH WALTER ,  GESSULAT HORST ,  RAMM PETER

Inventor： BEHLICH WALTER ,  GESSULAT HORST ,  RAMM PETER

IPC: B63C3/08

公开(公告)号：CA2157755A1

公开(公告)日：1997-03-08

申请号：CA2157755

申请日：1995-09-07

Applicant: RAMM PETER

Inventor： RAMM PETER

IPC: H04N5/351 ,  H04N5/372 ,  H04N5/335

Abstract: An electronic imaging system is disclosed which provides a very high level of sensitivity to enable imaging of biological and chemical specimens at low light levels. The system includes an integrating cooled CCD camera which has an image intensifier coupled thereto. With the present invention, light from the specimen is amplified by the intensifier, and the amplified light is integrated onto the cooled CCD camera over a period of time lasting for at least one second. At the end of the integration period, the camera is read out to a dedicated controller or imaging apparatus to create a digital image of the specimen. Frame averaging can be used within the imaging apparatus or controller, to improve the signal to noise ratio and increase the dynamic range of the camera. In addition, shading correction can be applied to remove spatial variations in camera sensitivity, to provide a homogeneous image background.

公开(公告)号：CH26193A

公开(公告)日：1903-08-15

申请号：CH26193D

申请日：1902-04-15

Applicant: RAMM PETER ,  BEHRENDT FRIEDRICH

Inventor： RAMM PETER ,  BEHRENDT FRIEDRICH