公开(公告)号：WO03095986A8

公开(公告)日：2005-05-06

申请号：PCT/IB0301821

申请日：2003-05-09

Applicant: AMERSHAM BIOSCIENCES NIAGARA I ,  RAMM PETER ,  ALEXAMDROV YURIY ,  CYBUCH JERRY ,  DONDERS PAUL ,  ZARATE CARLOS ,  SOLTYS BOHDAN J

Inventor： RAMM PETER ,  ALEXAMDROV YURIY ,  CYBUCH JERRY ,  DONDERS PAUL ,  ZARATE CARLOS ,  SOLTYS BOHDAN J

IPC: G01N15/00 ,  G01N15/14 ,  G01N21/17 ,  G01N21/64 ,  G01N33/48 ,  G06T1/00 ,  G01N33/50 ,  G02B21/24

CPC classification number: G01N21/6458 ,  G01N15/1475 ,  G01N21/6428 ,  G01N33/5026 ,  G01N2015/1497 ,  G01N2333/4603 ,  G06K9/00127 ,  G06T7/0012 ,  G06T7/187 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A system for performing automated cell screening in drug discovery includes an automated microscope (100), a fast autofocus device, and a digital imaging system (500). Processes are implemented in software through which relevant cellular material is segmented and quantified with minimal user interaction. Improvements in the following areas: known methods for image processing are implemented in such a way that automated segmentation is achieved; sets of known measurements (pixel counting, etc.) are implemented as methods which demonstrate aspects of biology in a reliable fashion; components for automated positioning, focusing, imaging and processing of a multiplicity of samples are integrated as systems within which the segmentation and measurement methods may be mounted; and components and methods are adapted into systems which yield more highly automated and more rapid cell screening.

Abstract translation: 用于在药物发现中进行自动细胞筛选的系统包括自动显微镜（100），快速自动聚焦装置和数字成像系统（500）。 过程在软件中实现，通过该软件，相关的细胞材料通过最小的用户交互被分段和量化。 以下领域的改进：图像处理的已知方法以实现自动分割的方式实现; 已知测量的集合（像素计数等）被实现为以可靠的方式示出生物学方面的方法; 将多个样本的自动定位，聚焦，成像和处理的组件集成为其中可以安装分割和测量方法的系统; 并且组件和方法被适应成产生更高度自动化和更快速的细胞筛选的系统。

公开(公告)号：WO0165461A2

公开(公告)日：2001-09-07

申请号：PCT/IB0100297

申请日：2001-03-02

Applicant: IMAGING RES INC ,  RAMM PETER ,  NADON ROBERT ,  SHI PEIDE

Inventor： RAMM PETER ,  NADON ROBERT ,  SHI PEIDE

IPC: G01N33/53 ,  C12Q1/68 ,  G01N37/00 ,  G06F17/18 ,  G06F19/20 ,  G06F19/24 ,  G06Q50/00 ,  G06F19/00

CPC classification number: G06F19/20 ,  C12Q1/6837 ,  G06F19/24

Abstract: An analytical process is disclosed, for discriminating data acquired from samples with overlapping distributions, and for improving and assessing the statistical validity of hybridization signal in arrays of assays. The process includes method of convolving data into two or more discrete probability density functions representing signal and nonsignal, discrete fluors, or other convolved independent variables. The system uses the probability density functions to assign hybridization signals, objectively, to one of the modeled distributions. Subsequent processes assess variability inherent to the arrays, and use this assessed variation to establish reliability scores and confidence limits for complete hybridization arrays, and for discrete hybridization assays within arrays.

Abstract translation: 公开了一种分析方法，用于区分从具有重叠分布的样品获得的数据，以及用于改进和评估测定阵列中杂交信号的统计学效度。 该过程包括将数据卷积成表示信号和非信号离散荧光或其它卷积的独立变量的两个或更多个离散概率密度函数的方法。 系统使用概率密度函数将杂交信号客观地分配给一个建模分布。 随后的过程评估阵列固有的变异性，并使用该评估的变异来建立完整杂交阵列的可靠性评分和置信限，以及阵列内的离散杂交测定。

公开(公告)号：DD143581A1

公开(公告)日：1980-09-03

申请号：DD21298179

申请日：1979-05-18

Applicant: BEHLICH WALTER ,  GESSULAT HORST ,  RAMM PETER

Inventor： BEHLICH WALTER ,  GESSULAT HORST ,  RAMM PETER

IPC: B63C3/08

公开(公告)号：WO02068942A3

公开(公告)日：2003-01-03

申请号：PCT/IB0200590

申请日：2002-02-27

Applicant: IMAGING RES INC ,  ZARATE CARLOS ,  DONDERS PAUL ,  YEKTA AHMED ,  MASOUMI ZAHRA ,  RAMM PETER

Inventor： ZARATE CARLOS ,  DONDERS PAUL ,  YEKTA AHMED ,  MASOUMI ZAHRA ,  RAMM PETER

IPC: G01N21/64 ,  G01N21/78 ,  G01N33/483

CPC classification number: G01N21/6408 ,  G01N2201/0648

Abstract: The present invention relates to an apparatus and method for imaging time resolved fluorescence in biochemical and medical samples. In a primary aspect, the device includes a lens of large aperture, a flash lamp in the illumination path, a fast-acting solid state shutter or a gated detector in the emission path, a device for delivering homogenous monochromatic illumination to a plurality of wells distributed within a microwell plate, a digital camera of high quantum efficiency, and a computer. under computer control, the lamp is pulsed at short intervals. The fast-acting emission shutter or gated detector operates to limit exposure of the camera to a period some microseconds after the extinction of each lamp pulse, during which only delayed fluorescence is transmitted to the camera. The invention achieves simultaneous time resolved imaging of a plurality of samples, with high sensitivity and high throughput.

Abstract translation: 本发明涉及用于在生物化学和医学样品中成像时间分辨荧光的装置和方法。 在主要方面，该装置包括大孔径的透镜，照明路径中的闪光灯，发射路径中的快速固态快门或门控检测器，用于将均匀单色照明传送到多个孔的装置 分布在微孔板，高量子效率的数码相机和计算机中。 在计算机控制下，灯以短时间脉冲。 快速发射快门或门控检测器操作以将相机的曝光限制在每个灯脉冲消失之后几微秒的时段，在此期间仅将延迟的荧光传输到相机。 本发明实现多个样品的同时时间分辨成像，具有高灵敏度和高产量。

公开(公告)号：WO0103189A8

公开(公告)日：2001-05-17

申请号：PCT/EP0006132

申请日：2000-06-30

Applicant: FRAUNHOFER GES FORSCHUNG ,  LANDESBERGER CHRISTOF ,  REICHL HERBERT ,  ANSORGE FRANK ,  RAMM PETER ,  EHRMANN OSWIN

Inventor： LANDESBERGER CHRISTOF ,  REICHL HERBERT ,  ANSORGE FRANK ,  RAMM PETER ,  EHRMANN OSWIN

IPC: H01L25/18 ,  H01L21/301 ,  H01L21/78 ,  H01L21/98 ,  H01L23/52 ,  H01L25/065 ,  H01L25/07

CPC classification number: H01L21/6836 ,  H01L21/78 ,  H01L24/24 ,  H01L24/82 ,  H01L25/0652 ,  H01L25/0657 ,  H01L25/50 ,  H01L2221/68327 ,  H01L2221/6834 ,  H01L2224/24051 ,  H01L2224/24226 ,  H01L2224/32225 ,  H01L2224/73267 ,  H01L2225/0651 ,  H01L2225/06517 ,  H01L2225/06524 ,  H01L2225/06541 ,  H01L2225/06555 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01032 ,  H01L2924/01033 ,  H01L2924/01052 ,  H01L2924/01061 ,  H01L2924/01068 ,  H01L2924/01075 ,  H01L2924/014 ,  H01L2924/10329 ,  H01L2924/13055 ,  H01L2924/14 ,  H01L2924/30105 ,  H01L2924/351 ,  H01L2924/00

Abstract: The invention relates to a multichip module comprising a base chip (10), a top chip (16), whose thickness is less than 100 mu m, and an adhesive layer (22) which is placed between the base chip (10) and the top chip (16), and both chips are mechanically joined to one another. An electrically insulating planarization layer (24) is provided such that the top chip (16) is embedded in the planarization layer (24). A through hole (26a) in the planarization layer (24) is filled with an electrically conductive material, is connected, in an electrically conductive manner, to a connection surface of the base chip (10), and is connected, in an electrically conductive manner, to a connection surface (20) of the top chip (16) via a metallization (28, 30). Through holes (26a, 26b) in the planarization layer (24) enable the production of all connections between the chips, between the base chip or between the top chip and external contacts of the multichip module in a manner that is independent of the individual chips and while using conventional semiconductor processing steps.

Abstract translation: 一种多芯片模块包括基底芯片（10）和顶片（16）之间的基片（10），顶片（16），厚度小于100微米，和粘合层（22）， 两个芯片机械连接在一起。 电绝缘平面化层（24）被设置成使得在所述平坦化层（24）的顶片（16）嵌入。 在平坦化层的通孔（26a）的（24）中填充有导电材料并在一方面与基底芯片（10）的连接表面（20）和，另一方面，经由金属化（28,30）同一个连接表面（20） 顶片（16）导电连接。 通过通孔（26A，26B）在平坦化层（24），可以在基体之间的芯片之间的所有连接，管芯或顶片和使用传统的半导体处理步骤的多芯片模块的外部接触，独立于单个芯片之间 生产。

公开(公告)号：WO1998028075A1

公开(公告)日：1998-07-02

申请号：PCT/IB1997001585

申请日：1997-12-18

Applicant: IMAGING RESEARCH INC. ,  RAMM, Peter

Inventor： IMAGING RESEARCH INC.

IPC: B01L03/00

CPC classification number: G01N21/03 ,  B01J2219/00317 ,  B01L3/5085 ,  B01L2300/0893 ,  C40B60/14 ,  G01N21/251 ,  G01N21/64 ,  G01N21/76 ,  G01N2021/0346 ,  G01N2021/0378

Abstract: A specimen containment system is disclosed which optimizes the optical properties of the specimen holders (wells) for imaging in order to enhance the relevant signal, relative to background. A family of well plates all have the inherent properties of: i) minimizing reflections from an excitation source into an imaging detector; ii) minimizing the concentration of excitation into intense and localized sources (lensing); and iii) allowing an epi-illuminating excitation beam to penetrate both central and lateral wells effectively. The well plates are made of a material that has or is treated to have minimal inherent fluorescence and minimal reflection properties. The well chambers are constructed to be depth-wise asymmetric (for example, sloping well surfaces) and to have cross-sectional irregularity. The bottom walls of the wells may also be formed with a ridge or made transparent so as to minimize reflections of incident light therefrom.

Abstract translation: 公开了一种样品容纳系统，其优化用于成像的样品保持器（孔）的光学性质，以便相对于背景增强相关信号。 一系列井板都具有固有的特性：i）最小化从激发源到成像探测器的反射; ii）将激发的浓度最小化为强烈和局部源（透镜）; 和iii）允许外照射激发光束有效地穿透中心阱和侧向阱。 孔板由具有或被处理为具有最小固有荧光和最小反射性质的材料制成。 井室被构造为深度不对称的（例如，倾斜的井表面）并且具有横截面的不规则性。 孔的底壁也可以形成有脊或制成透明的，以便使来自其的入射光的反射最小化。

公开(公告)号：WO1998007022A1

公开(公告)日：1998-02-19

申请号：PCT/US1997015269

申请日：1997-08-12

Applicant: IMAGING RESEARCH, INC. ,  RAMM, Peter ,  SUN, Gang ,  MUELLER, Rolf ,  ORMSBY, Timothy ,  CASTLE, Kenneth, R.

Inventor： IMAGING RESEARCH, INC.

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6456 ,  G01N21/76 ,  G02B13/14 ,  G02B13/22 ,  G02B21/084 ,  G06T7/75 ,  G06T2207/10056 ,  G06T2207/30072

Abstract: An electronic imaging system is disclosed, for assessing the intensity of colorimetric, fluorescent or luminescent signal in a matrix consisting of wells, microwells, hybridization dot blots on membranes, gels, or other specimens. The system includes a very sensitive area CCD detector (18), a fast, telecentric lens (22) with epi-illumination (44), a reflective/transmissive illumination system, an illumination wavelength selection device (34), and a light-tight chamber (24). A computer and image analysis software are used to control the hardware, correct and calibrate the images, and detect and quantify targets within the images.

Abstract translation: 公开了一种电子成像系统，用于评估由孔，微孔，膜，凝胶或其它样品上的杂交点印迹组成的基质中的比色，荧光或发光信号的强度。 该系统包括非常敏感的区域CCD检测器（18），具有外露照明（44）的快速，远心透镜（22），反射/透射照明系统，照明波长选择装置（34）和不透光 室（24）。 计算机和图像分析软件用于控制硬件，校正和校准图像，并检测和量化图像中的目标。

公开(公告)号：CH26088A

公开(公告)日：1903-07-31

申请号：CH26088D

申请日：1902-03-17

Applicant: RAMM PETER JOSEPH ,  BEHRENDT FRIEDRICH

Inventor： PETER JOSEPH RAMM ,  BEHRENDT FRIEDRICH

公开(公告)号：FR321214A

公开(公告)日：1903-01-05

申请号：FR321214D

申请日：1902-05-17

Applicant: RAMM PETER JOSEPH ,  BEHRENDT FRIEDRICH

Inventor： JOSEPH RAMM PETER ,  FRIEDRICH BEHRENDT

IPC: E03D5/08

公开(公告)号：CA2157755A1

公开(公告)日：1997-03-08

申请号：CA2157755

申请日：1995-09-07

Applicant: RAMM PETER

Inventor： RAMM PETER

IPC: H04N5/351 ,  H04N5/372 ,  H04N5/335

Abstract: An electronic imaging system is disclosed which provides a very high level of sensitivity to enable imaging of biological and chemical specimens at low light levels. The system includes an integrating cooled CCD camera which has an image intensifier coupled thereto. With the present invention, light from the specimen is amplified by the intensifier, and the amplified light is integrated onto the cooled CCD camera over a period of time lasting for at least one second. At the end of the integration period, the camera is read out to a dedicated controller or imaging apparatus to create a digital image of the specimen. Frame averaging can be used within the imaging apparatus or controller, to improve the signal to noise ratio and increase the dynamic range of the camera. In addition, shading correction can be applied to remove spatial variations in camera sensitivity, to provide a homogeneous image background.