公开(公告)号：DE60028665T2

公开(公告)日：2007-06-06

申请号：DE60028665

申请日：2000-08-03

Applicant: THK CO LTD ,  RIKEN WAKO

Inventor： NAKAZAWA TOJI ,  IIMURA AKIHIRO ,  TASHIRO HIDEO ,  KITSUNAI TOKUJI ,  HAYASHIZAKI YOSHIHIDE

IPC: B01J19/00 ,  C12M1/00 ,  B01L3/02 ,  C40B60/14 ,  G01N35/10

Abstract: The present invention relates to a micro array manufacturing apparatus, comprising: a working table 11 on which a plurality of substrates are arranged; a first solution storing portion 12 for storing a solution 53 including a living body sample; hold means for taking in said solution from said first solution storing portion 12 to form the spot of said solution on each of said substrates; moving means for supporting said hold means and moving said hold means in directions where said hold means approaches and retreats from said substrates, thereby allowing said hold means to form said solution spot; and delivery means for supporting said moving means and delivering said moving means in an area including said working table and said solution storing portion to provide two-dimensional coordinates; wherein said hold means comprises a second solution storing portion 52 having a pipe shape for storing a quantity of said solution to form the spots successively, the second solution storing portion 52 having an opening on one end thereof, and a needle 51 having a leading end portion stored in said second solution storing portion 52, the leading end portion being projectable from said second storing portion 52 by said moving means through the opening to allow said solution stored in said second solution storing portion to adhere onto each of said substrates by a given quantity.

公开(公告)号：DE60028665D1

公开(公告)日：2006-07-27

申请号：DE60028665

申请日：2000-08-03

Applicant: THK CO LTD ,  RIKEN WAKO

Inventor： NAKAZAWA THK CO ,  IIMURA AKIHIRO ,  TASHIRO HIDEO ,  KITSUNAI TOKUJI ,  HAYASHIZAKI YOSHIHIDE

IPC: B01J19/00 ,  C12M1/00 ,  B01L3/02 ,  C40B60/14 ,  G01N35/10

Abstract: The present invention relates to a micro array manufacturing apparatus, comprising: a working table 11 on which a plurality of substrates are arranged; a first solution storing portion 12 for storing a solution 53 including a living body sample; hold means for taking in said solution from said first solution storing portion 12 to form the spot of said solution on each of said substrates; moving means for supporting said hold means and moving said hold means in directions where said hold means approaches and retreats from said substrates, thereby allowing said hold means to form said solution spot; and delivery means for supporting said moving means and delivering said moving means in an area including said working table and said solution storing portion to provide two-dimensional coordinates; wherein said hold means comprises a second solution storing portion 52 having a pipe shape for storing a quantity of said solution to form the spots successively, the second solution storing portion 52 having an opening on one end thereof, and a needle 51 having a leading end portion stored in said second solution storing portion 52, the leading end portion being projectable from said second storing portion 52 by said moving means through the opening to allow said solution stored in said second solution storing portion to adhere onto each of said substrates by a given quantity.

公开(公告)号：DE60045451D1

公开(公告)日：2011-02-10

申请号：DE60045451

申请日：2000-08-03

Applicant: RIKEN ,  THK CO LTD

Inventor： NAKAZAWA TOJI ,  IIMURA AKIHIRO ,  TASHIRO HIDEO ,  KITSUNAI TOKUJI ,  HAYASHIZAKI YOSHIHIDE

IPC: B01J19/00 ,  C12M1/00 ,  B01L3/02 ,  C40B60/14 ,  G01N35/10

公开(公告)号：CA2363820A1

公开(公告)日：2002-05-24

申请号：CA2363820

申请日：2001-11-23

Applicant: RIKEN ,  UNIV WASEDA

Inventor： KONDOH YASUMITSU ,  TASHIRO HIDEO ,  KITSUNAI TOKUJI ,  NOJIMA TAKAHIKO ,  TAKENAKA SHIGEORI ,  MATSUMOTO KAZUKO

IPC: G01N31/22 ,  B01J19/00 ,  C07K1/04 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/53 ,  G01N37/00 ,  G01N33/531 ,  G01N33/543

Abstract: A biomolecule microarray support for spotting solutions containing probe biomolecules on the surface and immobilizing the probe biomolecules in the solutions to the surface, characterized in that a plurality of small-sized probe biomolecule-attachable spots are arrayed in a regular arrangement on the surface of the support. A biomolecule microarray, made by spotting solutions containing probe biomolecules on the biomolecule-attachable spots on the support and immobilizing the biomolecules to the spots, can be used for quantitative analysis because of uniform and unvarying numbers of probe biomolecules bound to the spots. It also makes possible high S/N ratio measurement because of prevented nonspecific adsorption of target biomolecules to the surface of the support other than the detection spots. The biomolecule microarray support is fabricated precisely and efficiently by the method of the present invention using the photolithography and etching technique.

公开(公告)号：CA2358011A1

公开(公告)日：2002-03-26

申请号：CA2358011

申请日：2001-09-24

Applicant: RIKEN

Inventor： KITSUNAI TOKUJI ,  TASHIRO TOMOKO ,  KONDOH YASUMITSU ,  TASHIRO HIDEO

IPC: G01N33/53 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/58 ,  G01N37/00 ,  G01N35/00 ,  C12Q1/00 ,  G01N33/533

Abstract: An integrated biomolecule sensor comprising a plurality of optical fibers whose proximal ends are held together with the end-faces arranged substantially in the same plane and oriented substantially in the same direction and which have probe polymers with different base sequences bound to the core end-face at their distal ends. The method and apparatus for fabricating the integrated biomolecule sensor, and the method and apparatus for detecting biomolecules using the integrated biomolecule sensor are also disclosed. Researchers can make their own sensors best suited for individual requirements, and detect the presence of target biomolecules quickly, without need of expensive equipment.

公开(公告)号：EP1643248A4

公开(公告)日：2008-05-14

申请号：EP04745956

申请日：2004-06-09

Applicant: RIKEN

Inventor： TASHIRO HIDEO ,  KONDOH YASUMITSU ,  KITSUNAI TOKUJI ,  HATAKEYAMA SATORU

IPC: G01N33/53 ,  B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/40 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  G01N21/25 ,  G01N21/64 ,  G01N21/78 ,  G01N33/543 ,  G01N37/00

CPC classification number: G01N21/6452 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5085 ,  C40B40/06 ,  C40B40/10 ,  G01N21/253 ,  G01N21/6428 ,  G01N33/5438 ,  G01N2021/6482

Abstract: A substrate for a biomolecule microarray having one or more spots for biomolecule immobilization. Each spot projects from the surface of the substrate and has a flat surface for a spot at the top, and at least the areas of the substrate around the projecting spots, a projecting spot side surface, and the flat surface are made of a conductive material. Alternatively, each spot projects from the surface of the substrate and has a flat surface for a spot at the top, adjacent projecting spots are jointed with their projecting spot side surfaces, and at least projecting spot side surface and the flat surface are made of a conductive material. A biomolecule interaction acceleration device is also disclosed which has an electrode so disposed as to face the surface of the microarray on which a biomolecule immobilization spot is provided and a power supply for applying an electric field between the microarray and the electrode.