公开(公告)号：CA2364324A1

公开(公告)日：2002-06-08

申请号：CA2364324

申请日：2001-12-05

Applicant: UNIV WASEDA ,  RIKEN

Inventor： MATSUMOTO KAZUKO ,  TASHIRO HIDEO ,  KONDOH YASUMITSU ,  NOJIMA TAKAHIKO ,  TAKENAKA SHIGEORI

IPC: G01N33/53 ,  C09B57/08 ,  C09B57/10 ,  C12Q1/68 ,  G01N21/75 ,  G01N33/566 ,  G01N33/58 ,  G01N33/533 ,  G01N33/544

Abstract: The present invention provides a novel detecting reagent for double-stranded nucleic acid, and a method of using it to detect double-stranded nucleic acid formed by hybridization with a probe, with absolutely no labeling of the target nucleic acid. The detecting reagent for double-stranded nucleic acid of the invention is characterized by comprising, in the same molecule, a naphthalenediimide group which is intercalatable into double-stranded nucleic acid and a .beta.- diketone group capable of forming a lanthanoid metal complex.

公开(公告)号：CA2363820A1

公开(公告)日：2002-05-24

申请号：CA2363820

申请日：2001-11-23

Applicant: RIKEN ,  UNIV WASEDA

Inventor： KONDOH YASUMITSU ,  TASHIRO HIDEO ,  KITSUNAI TOKUJI ,  NOJIMA TAKAHIKO ,  TAKENAKA SHIGEORI ,  MATSUMOTO KAZUKO

IPC: G01N31/22 ,  B01J19/00 ,  C07K1/04 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/53 ,  G01N37/00 ,  G01N33/531 ,  G01N33/543

Abstract: A biomolecule microarray support for spotting solutions containing probe biomolecules on the surface and immobilizing the probe biomolecules in the solutions to the surface, characterized in that a plurality of small-sized probe biomolecule-attachable spots are arrayed in a regular arrangement on the surface of the support. A biomolecule microarray, made by spotting solutions containing probe biomolecules on the biomolecule-attachable spots on the support and immobilizing the biomolecules to the spots, can be used for quantitative analysis because of uniform and unvarying numbers of probe biomolecules bound to the spots. It also makes possible high S/N ratio measurement because of prevented nonspecific adsorption of target biomolecules to the surface of the support other than the detection spots. The biomolecule microarray support is fabricated precisely and efficiently by the method of the present invention using the photolithography and etching technique.

公开(公告)号：CA2358011A1

公开(公告)日：2002-03-26

申请号：CA2358011

申请日：2001-09-24

Applicant: RIKEN

Inventor： KITSUNAI TOKUJI ,  TASHIRO TOMOKO ,  KONDOH YASUMITSU ,  TASHIRO HIDEO

IPC: G01N33/53 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/77 ,  G01N33/543 ,  G01N33/58 ,  G01N37/00 ,  G01N35/00 ,  C12Q1/00 ,  G01N33/533

Abstract: An integrated biomolecule sensor comprising a plurality of optical fibers whose proximal ends are held together with the end-faces arranged substantially in the same plane and oriented substantially in the same direction and which have probe polymers with different base sequences bound to the core end-face at their distal ends. The method and apparatus for fabricating the integrated biomolecule sensor, and the method and apparatus for detecting biomolecules using the integrated biomolecule sensor are also disclosed. Researchers can make their own sensors best suited for individual requirements, and detect the presence of target biomolecules quickly, without need of expensive equipment.

公开(公告)号：EP1643248A4

公开(公告)日：2008-05-14

申请号：EP04745956

申请日：2004-06-09

Applicant: RIKEN

Inventor： TASHIRO HIDEO ,  KONDOH YASUMITSU ,  KITSUNAI TOKUJI ,  HATAKEYAMA SATORU

IPC: G01N33/53 ,  B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/40 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  G01N21/25 ,  G01N21/64 ,  G01N21/78 ,  G01N33/543 ,  G01N37/00

CPC classification number: G01N21/6452 ,  B01J19/0046 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5085 ,  C40B40/06 ,  C40B40/10 ,  G01N21/253 ,  G01N21/6428 ,  G01N33/5438 ,  G01N2021/6482

Abstract: A substrate for a biomolecule microarray having one or more spots for biomolecule immobilization. Each spot projects from the surface of the substrate and has a flat surface for a spot at the top, and at least the areas of the substrate around the projecting spots, a projecting spot side surface, and the flat surface are made of a conductive material. Alternatively, each spot projects from the surface of the substrate and has a flat surface for a spot at the top, adjacent projecting spots are jointed with their projecting spot side surfaces, and at least projecting spot side surface and the flat surface are made of a conductive material. A biomolecule interaction acceleration device is also disclosed which has an electrode so disposed as to face the surface of the microarray on which a biomolecule immobilization spot is provided and a power supply for applying an electric field between the microarray and the electrode.