公开(公告)号：WO2006036757A3

公开(公告)日：2006-04-06

申请号：PCT/US2005/034045

申请日：2005-09-23

Applicant: SANDIA NATIONAL LABORATORIES ,  WEST, Jason, A., A. ,  HUKARI, Kyle, W. ,  PATEL, Kamlesh, D. ,  PETERSON, Kenneth, A.

Inventor： WEST, Jason, A., A. ,  HUKARI, Kyle, W. ,  PATEL, Kamlesh, D. ,  PETERSON, Kenneth, A.

IPC: C12M1/33 ,  B01L3/00 ,  C12N1/06

Abstract: Devices (100) and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers (100) coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

公开(公告)号：WO2009131722A2

公开(公告)日：2009-10-29

申请号：PCT/US2009/031515

申请日：2009-01-21

Applicant: SANDIA NATIONAL LABORATORIES ,  PERROUD, Thomas, D. ,  PATEL, Kamlesh, D.

Inventor： PERROUD, Thomas, D. ,  PATEL, Kamlesh, D.

IPC: G01N1/28 ,  G01N35/10

CPC classification number: G01N33/5005 ,  B01F3/0807 ,  B01F5/0471 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0688 ,  B81B2201/058 ,  B81C1/00047 ,  B81C1/00087 ,  B81C1/00119 ,  B81C1/00404 ,  B81C2201/0133 ,  B81C2201/0142 ,  Y10T137/9464 ,  Y10T428/24479

Abstract: Disclosed herein are methods of immobilizing a particle which comprise focusing the flow of a sample fluid containing the particle into a virtual channel which flows towards an unoccupied hydrodynamic trap in a microfluidic channel such that the particle flows into the hydrodynamic trap and becomes immobilized therein. Also disclosed are microfluidic devices which comprise at least one microchannel having at least one hydrodynamic trap, at least one focusing fluid inlet, said focusing fluid inlet is upstream of the hydrodynamic trap such that a focusing fluid introduced therein results in a virtual channel of a sample fluid when present which preferentially flows toward the hydrodynamic trap.

Abstract translation: 本文公开了固定颗粒的方法，其包括将含有颗粒的样品流体的流动聚焦到虚拟通道中，该虚拟通道朝向微流体通道中的未占据的流体动力阱捕获，使得颗粒流入流体动力阱并固定在其中。 还公开了微流体装置，其包括具有至少一个流体动力捕集器，至少一个聚焦流体入口的至少一个微通道，所述聚焦流体入口在流体动力捕集器的上游，使得其中引入的聚焦流体导致样品的虚拟通道 存在时优先流向流体动力阱的流体。

公开(公告)号：WO2009126352A2

公开(公告)日：2009-10-15

申请号：PCT/US2009/031516

申请日：2009-01-21

Applicant: SANDIA NATIONAL LABORATORIES ,  PERROUD, Thomas, D. ,  PATEL, Kamlesh, D. ,  MEAGHER, Robert, J.

Inventor： PERROUD, Thomas, D. ,  PATEL, Kamlesh, D. ,  MEAGHER, Robert, J.

IPC: H01L21/311 ,  H01L21/02 ,  H01L21/70

CPC classification number: G01N33/5005 ,  B01F3/0807 ,  B01F5/0471 ,  B01F13/0059 ,  B01L3/502707 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502776 ,  B01L3/502784 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0688 ,  B81B2201/058 ,  B81C1/00047 ,  B81C1/00087 ,  B81C1/00119 ,  B81C1/00404 ,  B81C2201/0133 ,  B81C2201/0142 ,  Y10T137/9464 ,  Y10T428/24479

Abstract: Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2X a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.

Abstract translation: 这里公开的方法是在衬底中的两个各向同性湿蚀刻特征之间制造所需高度和/或宽度的微孔的方法，其包括使用蚀刻剂和小于2X的蚀刻剂的掩模距离的单层各向同性湿法蚀刻两个特征 深度。 本文还公开了使用包括微孔的微孔和微流体装置的方法。

公开(公告)号：EP1794281A2

公开(公告)日：2007-06-13

申请号：EP05809902.9

申请日：2005-09-23

Applicant: SANDIA NATIONAL LABORATORIES

Inventor： WEST, Jason, A., A. ,  HUKARI, Kyle, W. ,  PATEL, Kamlesh, D. ,  PETERSON, Kenneth, A.

IPC: C12M1/33

CPC classification number: B01L3/5027 ,  B01L7/00 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1827 ,  C12M47/06 ,  C12M47/20 ,  C12N1/066

Abstract: Devices (100) and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers (100) coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

Abstract translation: 提供了用于热裂解生物材料的装置和方法，例如营养细菌细菌和细菌孢子。 描述了溶解细菌孢子的热溶解方法。 公开了用于直接分析的系统，包括耦合到样品制备站的热裂解器。 提供了能够对生物材料进行样品裂解，标记和蛋白质指纹分析的集成系统，例如营养细菌细菌，细菌孢子和病毒。