公开(公告)号：DE3750094T2

公开(公告)日：1994-10-20

申请号：DE3750094

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：ES2054701T3

公开(公告)日：1994-08-16

申请号：ES87830033

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：IT1213313B

公开(公告)日：1989-12-20

申请号：IT2131486

申请日：1986-07-30

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12K

Abstract: A cloned and sequenced ECORI fragment of Bordetella pertussis chromosomal DNA with 4696 base pairs contains the genes which code for the 5 subunits of the pertussis toxin or further fragments, where the fragments contain at least one gene which codes for at least one subunit of the pertussis toxin, characterised by a given sequence. Also claimed is pertussis toxin in which the subunits S1,S2, S3, S4 are characterised by their aminoterminal sequences.

公开(公告)号：AT107692T

公开(公告)日：1994-07-15

申请号：AT87830033

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50 ,  C12N1/20

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：HK3396A

公开(公告)日：1996-01-12

申请号：HK3396

申请日：1996-01-04

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：DE3750094D1

公开(公告)日：1994-07-28

申请号：DE3750094

申请日：1987-01-28

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12N15/09 ,  A61K39/00 ,  A61K39/095 ,  A61K39/10 ,  C07K14/00 ,  C07K14/195 ,  C07K14/235 ,  C07K14/41 ,  C12N1/00 ,  C12N15/00 ,  C12N15/83 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: Cloning and sequencing of the EcoRI fragment of B. pertussis chromosomal DNA with 4696 base pairs, conta ining the genes which code for the five subunits of the pertussis toxin. A hybrid plasmid containing the DNA fragment or its further fragments and a micro-organism transformed by the hybrid plasmid and capable of expressing the cloned DNA fragment or further fragments thereof by synthesis of the pertussis toxin or one or more subunits of the pertussis toxin. The pertussis toxine or one or more subunits of the pertussis toxin so obtained are useful for the preparation of vaccines and diagnostic kits.

公开(公告)号：IT8621314D0

公开(公告)日：1986-07-30

申请号：IT2131486

申请日：1986-07-30

Applicant: SCLAVO SPA

Inventor： RAPPUOLI RINO ,  NICOSIA ALFREDO ,  ARICO MARIA BEATRICE

IPC: C12K