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1.发明申请METHOD OF EXTENDING SINGLE-STRANDED NUCLEIC ACID, SINGLE-STRANDED NUCLEIC ACID EXTENDING APPARATUS AND DNA CHIP 审中-公开扩增单链核酸，单链核酸扩增装置和DNA芯片的方法
公开(公告)号：WO2005033326A3
公开(公告)日：2005-05-19
申请号：PCT/JP2004015094
申请日：2004-10-06
Applicant: SONY CORP , MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
Inventor： MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6832 , C12Q2523/301 , C12Q2523/307
Abstract: The action of high-frequency AC electric field on a single-stranded nucleic acid being present in an aqueous solution is verified, and the action is utilized for enhancement of the efficiency of hybridization for conversion of the single-stranded nucleic acid to a companion complementary strand. There are provided, for example, a method of nucleic acid extension and nucleic acid extending apparatus, wherein high-frequency AC electric field is applied to a single-stranded nucleic acid being present in liberated form in pure water or an aqueous solution of 5 to 11 pH (R), or to a single-stranded nucleic acid fixed onto the surface (f) of one or both (E) of counter electrodes (E,E) provided so as to face the aqueous solution (R), thereby extending the single-stranded nucleic acid.
Abstract translation: 证实了高频AC电场对存在于水溶液中的单链核酸的作用，并且该作用被用于提高单链核酸转化为伴侣互补的杂交效率 链。 例如，提供了一种核酸延伸和核酸延伸装置的方法，其中将高频AC电场施加到以游离态形式存在的单链核酸在纯水或5至 （或E）对电极（E，E）的一个或两个表面（f）上固定的单链核酸，以便面对水溶液（R），从而延伸 单链核酸。
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2.发明公开METHOD OF EXTENDING SINGLE-STRANDED NUCLEIC ACID, SINGLE-STRANDED NUCLEIC ACID EXTENDING APPARATUS AND DNA CHIP 审中-公开方法单链NUCLEIC装置的延长单链NUCLEIC和DNA芯片的推广
公开(公告)号：EP1679369A4
公开(公告)日：2007-12-12
申请号：EP04792334
申请日：2004-10-06
Applicant: SONY CORP
Inventor： MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6832 , C12Q2523/301 , C12Q2523/307
Abstract: The action of high-frequency AC electric field on a single-stranded nucleic acid being present in an aqueous solution is verified, and the action is utilized for enhancement of the efficiency of hybridization for conversion of the single-stranded nucleic acid to a companion complementary strand. There are provided, for example, a method of nucleic acid extension and nucleic acid extending apparatus, wherein high-frequency AC electric field is applied to a single-stranded nucleic acid being present in liberated form in pure water or an aqueous solution of 5 to 11 pH (R), or to a single-stranded nucleic acid fixed onto the surface (f) of one or both (E) of counter electrodes (E,E) provided so as to face the aqueous solution (R), thereby extending the single-stranded nucleic acid.
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3.发明专利Method for elongating single strand nucleic acid and apparatus for elongating single strand nucleic acid and dna chip 审中-公开用于制备单丝核酸的方法和用于制备单丝核酸和DNA芯片的装置
公开(公告)号：JP2005110539A
公开(公告)日：2005-04-28
申请号：JP2003346779
申请日：2003-10-06
Inventor： MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6832 , C12Q2523/301 , C12Q2523/307
Abstract: PROBLEM TO BE SOLVED: To verify the action of a high frequency AC electric field on a single strand nucleic acid existing in an aqueous solution never containing an ingredient such as a nucleic acid material, an enzyme, or a primer for synthesizing DNA, and to use the action for improving the efficiency of a hybridization using the single strand nucleic acid as a complementary chain for the other nucleic acid.
SOLUTION: This method for elongating the single strand nucleic acid, comprises making a high frequency AC electric field to act on a single strand nucleic acid existing in pure water or an aqueous solution (R) of pH 15 to 11, or a single strand nucleic acid immobilized on the surface or surfaces (f) of one or both of counter electrodes (E, E) adjacently disposed to the aqueous solution (R). And an apparatus for elongating the single strand nucleic acid, and the like are provided.
COPYRIGHT: (C)2005,JPO&NCIPIAbstract translation: 要解决的问题：为了验证高频AC电场对存在于不含有诸如用于合成DNA的核酸材料，酶或引物的成分的水溶液中的单链核酸的作用 并且使用该作用来提高使用单链核酸作为其它核酸的互补链的杂交效率。 解决方案：用于延长单链核酸的方法包括使高频AC电场作用于存在于纯水或pH 15至11的水溶液（R）中的单链核酸，或 单链核酸固定在相邻设置于水溶液（R）的相对电极（E，E）中的一个或两个的表面或表面（f）上。 并且提供了用于延长单链核酸的装置等。 版权所有（C）2005，JPO＆NCIPI
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