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1.发明申请METHOD OF EXTENDING SINGLE-STRANDED NUCLEIC ACID, SINGLE-STRANDED NUCLEIC ACID EXTENDING APPARATUS AND DNA CHIP 审中-公开扩增单链核酸，单链核酸扩增装置和DNA芯片的方法
公开(公告)号：WO2005033326A3
公开(公告)日：2005-05-19
申请号：PCT/JP2004015094
申请日：2004-10-06
Applicant: SONY CORP , MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
Inventor： MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6832 , C12Q2523/301 , C12Q2523/307
Abstract: The action of high-frequency AC electric field on a single-stranded nucleic acid being present in an aqueous solution is verified, and the action is utilized for enhancement of the efficiency of hybridization for conversion of the single-stranded nucleic acid to a companion complementary strand. There are provided, for example, a method of nucleic acid extension and nucleic acid extending apparatus, wherein high-frequency AC electric field is applied to a single-stranded nucleic acid being present in liberated form in pure water or an aqueous solution of 5 to 11 pH (R), or to a single-stranded nucleic acid fixed onto the surface (f) of one or both (E) of counter electrodes (E,E) provided so as to face the aqueous solution (R), thereby extending the single-stranded nucleic acid.
Abstract translation: 证实了高频AC电场对存在于水溶液中的单链核酸的作用，并且该作用被用于提高单链核酸转化为伴侣互补的杂交效率 链。 例如，提供了一种核酸延伸和核酸延伸装置的方法，其中将高频AC电场施加到以游离态形式存在的单链核酸在纯水或5至 （或E）对电极（E，E）的一个或两个表面（f）上固定的单链核酸，以便面对水溶液（R），从而延伸 单链核酸。
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2.发明公开METHOD OF EXTENDING SINGLE-STRANDED NUCLEIC ACID, SINGLE-STRANDED NUCLEIC ACID EXTENDING APPARATUS AND DNA CHIP 审中-公开方法单链NUCLEIC装置的延长单链NUCLEIC和DNA芯片的推广
公开(公告)号：EP1679369A4
公开(公告)日：2007-12-12
申请号：EP04792334
申请日：2004-10-06
Applicant: SONY CORP
Inventor： MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6832 , C12Q2523/301 , C12Q2523/307
Abstract: The action of high-frequency AC electric field on a single-stranded nucleic acid being present in an aqueous solution is verified, and the action is utilized for enhancement of the efficiency of hybridization for conversion of the single-stranded nucleic acid to a companion complementary strand. There are provided, for example, a method of nucleic acid extension and nucleic acid extending apparatus, wherein high-frequency AC electric field is applied to a single-stranded nucleic acid being present in liberated form in pure water or an aqueous solution of 5 to 11 pH (R), or to a single-stranded nucleic acid fixed onto the surface (f) of one or both (E) of counter electrodes (E,E) provided so as to face the aqueous solution (R), thereby extending the single-stranded nucleic acid.
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Patent Agency Ranking3.发明专利Method for preventing nonspecific adsorption of fluorescent intercalator to solid phase surface, hybridization detection method using the method, and substrate for bioassay 审中-公开用于防止荧光相互作用体非固定吸附到固相表面的方法，使用该方法的杂化检测方法和用于生物传感的基质
公开(公告)号：JP2005331334A
公开(公告)日：2005-12-02
申请号：JP2004149226
申请日：2004-05-19
Inventor： MATSUMOTO SAYOKO , YAMASHITA YUKIISA , ONISHI MICHIHIRO , SATO KAZUMASA
IPC: G01N33/53 , G01N33/543 , G01N37/00
Abstract: PROBLEM TO BE SOLVED: To prevent effectively nonspecific adsorption of an intercalator onto the solid state surface.
SOLUTION: The solid state surface f for detecting hybridization between a nucleic acid D for detection and a target nucleic acid T is covered with a material to be positively charged such as avidin or modified with a functional group to be positively charged resulting from a silane coupling agent, to thereby prevent nonspecific adsorption of the fluorescent intercalator I onto the solid state surface f. Thus this method is especially useful when detecting hybridization on the solid state surface f by using the fluorescent intercalator I.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 要解决的问题：为了防止嵌入剂有效地非特异性地吸附到固态表面上。 解决方案：用于检测用于检测的核酸D和靶核酸T之间的杂交的固态表面f被被带正电的材料如抗生物素蛋白覆盖，或被官能团改性，由带正电的官能团 硅烷偶联剂，从而防止荧光嵌入剂I非固定吸附到固态表面f上。 因此，当使用荧光嵌入剂I检测固态表面f上的杂交时，该方法特别有用。（C）2006，JPO＆NCIPI
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4.发明专利Interaction detecting section and substrate for bioassay equipped with the same, and medium dropping method and method for preventing water in medium from evaporating 审中-公开相关检测部分和与之相关的生物传感器基板，以及用于防止来自蒸发器的介质中的水的中间浸渍方法和方法
公开(公告)号：JP2005300460A
公开(公告)日：2005-10-27
申请号：JP2004120468
申请日：2004-04-15
Inventor： MATSUMOTO SAYOKO , YUBI HIROSHI
IPC: G01N33/53 , G01N1/00 , G01N33/543 , G01N33/566 , G01N37/00
Abstract: PROBLEM TO BE SOLVED: To provide a technique or the like, capable of effectively preventing water in a medium which is reserved or held in a reaction region, from evaporating.
SOLUTION: An interaction detecting section 1 is provided with the reaction section 2 providing a field of interaction between matters; a capillary section 3 being in communication with the reaction section 2 and sending a hydrophile medium M to the reaction section 2 by utilizing capillary phenomenon. The interaction detecting section 1, a periphery region 31b of a sucking inlet 31a of the capillary section 3, an inner wall surface of the capillary 31 ranging from the sucking inlet 31a to the reaction section 2 and an inner wall surface of the reaction section 2 are made hydrophile. Furthermore, a substrate B for a bioassay is provided with the detection section 1.
COPYRIGHT: (C)2006,JPO&NCIPIAbstract translation: 要解决的问题：提供能够有效防止保留或保持在反应区域中的介质中的水不会蒸发的技术等。 解决方案：交互检测部分1设置有反应部分2，提供事件之间的相互作用领域; 毛细管部分3与反应部分2连通并且通过利用毛细管现象将亲水介质M发送到反应部分2。 相互作用检测部1，毛细管部3的吸入口31a的周边区域31b，毛细管31的从吸入口31a到反应部2的内壁面和反应部2的内壁面 被做成亲水。 此外，用于生物测定的基板B设置有检测部分1.版权所有（C）2006，JPO＆NCIPI
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5.发明专利Detection part of interaction between substances, bioassay substrate using it, bioassay device and bioassay method 审中-公开使用物质，生物传感器设备和生物传感器方法的物质，生物基质之间的相互作用的检测部分
公开(公告)号：JP2006177725A
公开(公告)日：2006-07-06
申请号：JP2004369976
申请日：2004-12-21
Inventor： SEGAWA YUJI , MATSUMOTO SAYOKO , KISHII NORIYUKI , ONISHI MICHIHIRO
IPC: G01N33/543 , G01N21/64 , G01N21/78 , G01N33/53 , G01N33/542 , G01N37/00
Abstract: PROBLEM TO BE SOLVED: To provide a novel technique which reduces the background noise and achieves enhancement of the S/N ratio. SOLUTION: This detection part is used when the specific or complementary interaction between a probe molecule X and a target molecule Y is detected, on the basis of the fluorescence signal from a fluorescent substance and equipped with at least a reaction region R for providing a place of the interaction and the detection surface D facing the reaction region R. The detection surface D provides the interaction detecting part 1, formed of a metal or an alloy for exciting surface plasmon, a substrate using the detection part 1, a device that uses using the detection part 1 and a method that uses the detection part 1. COPYRIGHT: (C)2006,JPO&NCIPI
Abstract translation: 要解决的问题：提供降低背景噪声并实现S / N比的提高的新型技术。 解决方案：当检测到探针分子X和靶分子Y之间的特异性或互补相互作用时，根据来自荧光物质的荧光信号并且至少配备有反应区域R的装置，使用该检测部分 提供相互作用的位置和面向反应区域R的检测表面D.检测表面D提供由用于激发表面等离子体激元的金属或合金形成的相互作用检测部分1，使用检测部分1的基板， 其使用检测部分1和使用检测部分1的方法。版权所有：（C）2006，JPO＆NCIPI
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公开(公告)号：JP2011258549A
公开(公告)日：2011-12-22
申请号：JP2011107447
申请日：2011-05-12
Inventor： SUGIYAMA HIROKI , KUMITA HIDEYUKI , MATSUMOTO SAYOKO , SAKAI HIDEKI , TOKITA YUICHI , SAGAWA TUNETOSHI , FUJITA SHUJI , YAMAGUCHI DAISUKE , MITA HIROKI , NAKAGAWA TAKAAKI
CPC classification number: Y02E60/527
Abstract: PROBLEM TO BE SOLVED: To provide a fuel container for a biofuel cell and a biofuel cell system, whereby fuel solution in a battery can be exchanged simply and conveniently without a need for a fuel supply mechanism such as a pump in a fuel cell body.SOLUTION: The fuel container including: a fuel solution storage part in which the fuel solution is stored; a waste liquid storage part in which waste liquid is stored; and a liquid feed mechanism by which the fuel solution is discharged and the solution after use is sucked is used to exchange fuel of the biofuel cell equipped with an electrode having oxygen existing on the surface. Concretely, the fuel solution stored in the fuel solution storage part of the fuel container is poured into the biofuel cell by the fuel feed mechanism, and the solution (waste liquid) after use in the biofuel cell is recovered in the waste liquid storage part.
Abstract translation: 要解决的问题：提供一种用于生物燃料电池和生物燃料电池系统的燃料容器，由此可以简单方便地更换电池中的燃料溶液，而不需要诸如燃料中的泵的燃料供应机构 细胞体。 解决方案：燃料容器包括：燃料溶液储存部分，其中存储有燃料溶液; 储存有废液的废液储存部; 以及吸入燃料溶液并使用后的溶液被吸入的液体供给机构，用于交换配备有表面存在氧的电极的生物燃料电池的燃料。 具体地说，储存在燃料容器的燃料溶液贮存部的燃料溶液通过燃料供给机构注入生物燃料电池，在生物燃料电池中使用后的溶液（废液）被回收到废液收容部。 版权所有（C）2012，JPO＆INPIT
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7.发明专利Method for elongating single strand nucleic acid and apparatus for elongating single strand nucleic acid and dna chip 审中-公开用于制备单丝核酸的方法和用于制备单丝核酸和DNA芯片的装置
公开(公告)号：JP2005110539A
公开(公告)日：2005-04-28
申请号：JP2003346779
申请日：2003-10-06
Inventor： MATSUMOTO SAYOKO , MAMINE TAKAYOSHI , WASHIZU MASAO , KUROSAWA OSAMU
CPC classification number: C12Q1/6837 , C12Q1/6806 , C12Q1/6832 , C12Q2523/301 , C12Q2523/307
Abstract: PROBLEM TO BE SOLVED: To verify the action of a high frequency AC electric field on a single strand nucleic acid existing in an aqueous solution never containing an ingredient such as a nucleic acid material, an enzyme, or a primer for synthesizing DNA, and to use the action for improving the efficiency of a hybridization using the single strand nucleic acid as a complementary chain for the other nucleic acid.
SOLUTION: This method for elongating the single strand nucleic acid, comprises making a high frequency AC electric field to act on a single strand nucleic acid existing in pure water or an aqueous solution (R) of pH 15 to 11, or a single strand nucleic acid immobilized on the surface or surfaces (f) of one or both of counter electrodes (E, E) adjacently disposed to the aqueous solution (R). And an apparatus for elongating the single strand nucleic acid, and the like are provided.
COPYRIGHT: (C)2005,JPO&NCIPIAbstract translation: 要解决的问题：为了验证高频AC电场对存在于不含有诸如用于合成DNA的核酸材料，酶或引物的成分的水溶液中的单链核酸的作用 并且使用该作用来提高使用单链核酸作为其它核酸的互补链的杂交效率。 解决方案：用于延长单链核酸的方法包括使高频AC电场作用于存在于纯水或pH 15至11的水溶液（R）中的单链核酸，或 单链核酸固定在相邻设置于水溶液（R）的相对电极（E，E）中的一个或两个的表面或表面（f）上。 并且提供了用于延长单链核酸的装置等。 版权所有（C）2005，JPO＆NCIPI
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