公开(公告)号：EP1908849A4

公开(公告)日：2009-03-04

申请号：EP06781776

申请日：2006-07-27

Applicant: SONY CORP

Inventor： NAKAMURA RYOTA ,  KISHII NORIYUKI ,  ABE TOMOTERU ,  YAMAMOTO TAKURO ,  YAMASHITA SHIKO ,  SEGAWA YUJI ,  OMORI SHINJI

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6832 ,  C12Q1/6837 ,  C12Q2563/173 ,  C12Q2527/101 ,  C12Q2521/307 ,  C12Q2527/107 ,  C12Q2523/307

Abstract: The object is to increase the S/N ratio in the detection of the occurrence of hybridization to increase the detection accuracy. Disclosed is a hybridization detection method, in which the hybridization between a probe nucleic acid chain with a target nucleic acid chain is allowed to proceed in a reaction system, and then the temperature of the reaction system where the hybridization proceeds is raised to a temperature higher than a predetermined temperature, thereby increasing the S/N ratio in the detection of the hybridization.

公开(公告)号：JP2007054048A

公开(公告)日：2007-03-08

申请号：JP2006170531

申请日：2006-06-20

Applicant: Sony Corp ,  ソニー株式会社

Inventor： SEGAWA YUJI ,  KISHII NORIYUKI ,  OMORI SHINJI ,  NAKAMURA RYOTA ,  ABE TOMOTERU ,  YAMAMOTO TAKUO ,  YAMASHITA SHIKO

IPC: C12Q1/68 ,  C12N15/09

CPC classification number: C12Q1/6832 ,  C12Q1/6837 ,  C12Q2563/173 ,  C12Q2527/101 ,  C12Q2521/307 ,  C12Q2527/107 ,  C12Q2523/307

Abstract: PROBLEM TO BE SOLVED: To increase the S/N ratio in the detection of the occurrence of hybridization to increase the detection accuracy.
SOLUTION: The hybridization between a probe nucleic acid chain and a target nucleic acid chain is allowed to proceed in a reaction system, and then the temperature of the reaction system where the hybridization proceeds is raised to a temperature higher than a predetermined temperature, thereby increasing the S/N ratio in the detection of the hybridization.
COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：为了提高杂交发生的检测中的S / N比，提高检测精度。 解决方案：使探针核酸链和靶核酸链之间的杂交在反应体系中进行，然后将杂交进行的反应体系的温度升高至高于预定温度的温度 ，从而提高杂交检测中的S / N比。 版权所有（C）2007，JPO＆INPIT