公开(公告)号：EP1873514A4

公开(公告)日：2012-12-12

申请号：EP06745396

申请日：2006-04-12

Applicant: SONY CORP

Inventor： OHTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/64 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/25 ,  G01N21/78 ,  G01N33/53 ,  G01N33/566

CPC classification number: G01N21/6452 ,  C12Q1/6816 ,  G01N21/253 ,  C12Q2565/501

公开(公告)号：JP2007017282A

公开(公告)日：2007-01-25

申请号：JP2005199016

申请日：2005-07-07

Applicant: Sony Corp ,  ソニー株式会社

Inventor： OTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/64 ,  C12M1/00 ,  G01N21/78 ,  G01N33/53 ,  G01N33/566 ,  G06N3/00

Abstract: PROBLEM TO BE SOLVED: To learn the judgment of the adoption or non-adoption of a spot based on reliability. SOLUTION: Developed profile data 744 including feature quantity data is provided to SVM 91 and the images of the respective spots of a developed profile image 741 are displayed on a display part. The indication related to whether the respective spots are set to adoption spots 742 or non-adoption spots 743 is received as the teacher data of a learning mode from a user. The non-adoption spots 743 are the spots judged not to be adopted because a large deburi or the like detracting the reliability of a measured result and the adoption spots 742 are the spots wherein deburi or the like is present and effective hybridization is produced. The SVM 91 learns the relation of the teacher data indicated by the user with the feature quantity data at every spot of the developed profile data 744 to store the learning result in a spot removing pattern database 92. This invention can be adapted to a biological data processor. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：根据可靠性了解采用或不采用现场的判断。 解决方案：将包括特征量数据的显影曲线数据744提供给SVM 91，并且显影轮廓图像741的各个点的图像显示在显示部分上。 作为用户的学习模式的教师数据，接收到各点是否设定为采用点742或非采用点743的指示。 非采用点743是判断为不被采用的点，因为损害了测量结果的可靠性的大型文件等，并且采用点742是存在其中存在有效杂质的斑点，并且产生了有效的杂交。 SVM 91学习用户指示的教师数据与所开发的简档数据744的每个位置的特征量数据的关系，以将学习结果存储在斑点去除模式数据库92中。本发明可以适用于生物数据 处理器。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006300798A

公开(公告)日：2006-11-02

申请号：JP2005124570

申请日：2005-04-22

Applicant: Sony Corp ,  ソニー株式会社

Inventor： OTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/64 ,  G01N21/78 ,  G01N33/53 ,  G01N33/566

Abstract: PROBLEM TO BE SOLVED: To sufficiently ensure a dynamic range of an estimable hybridized amount. SOLUTION: In a case that a plurality of developed profile images acquired by scanning the spot 12 provided on a DNA chip 11 by exciting lights different in intensity are set to a processing target, in a preparation part 81, hybridized amounts of which the number is the same as that of the developed profile images are acquired from the fluorescence intensities of the developed profile images and a preliminarily prepared fluorescence intensity-hybridized amount conversion formula. One hybridized amount is calculated by applying weighting to the acquired hybridized amounts and the calculated hybridized amount is set as the estimate value of the hybridized amount produced in the spot 12. This biological data processor is adapted to an apparatus for measuring the fluorescence intensity of the DNA chip. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：充分确保可估计杂交量的动态范围。 解决方案：在将通过激励强度不同的光照射到DNA芯片11上的斑点12获得的多个显影图像图像设置为处理目标的情况下，在准备部分81中，杂交量 与显影图像的荧光强度和初步制备的荧光强度 - 杂交量转换公式相比，数字与显影图像的数量相同。 通过对获得的杂交量应用加权来计算一个杂交量，并将计算的杂交量设置为在斑点12中产生的杂交量的估计值。该生物数据处理器适用于测量荧光强度的装置 DNA芯片 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006300797A

公开(公告)日：2006-11-02

申请号：JP2005124569

申请日：2005-04-22

Applicant: Sony Corp ,  ソニー株式会社

Inventor： OTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/78 ,  C12M1/40 ,  C12N15/00 ,  G01N33/53 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To know a developed amount from the intensity of fluorescence more accurately and quantitatively. SOLUTION: A fluorescence intensity acquiring part 22 receives the input of the fluorescence intensity of each of spots outputted from the photodiode 54 of a fluorescence intensity acquiring pickup 41. In a fluorescence intensity hybridized amount conversion formula calculating part 25, a fluorescence intensity-hybridized amount conversion formula for unitarily determining the relation of fluorescence intensity with the hybridized amount corresponding to it is calculated from the ratio of the probe and dummy probe in each of the spots stored in a spot position-mixing ratio memory part 26 and the image of each of the spot supplied from an image processing part 24. This invention can be adapted to this biological data processor for processing biological data. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：从荧光强度更准确和定量地了解发达量。 荧光强度获取部22接收从荧光强度获取拾取器41的光电二极管54输出的每个点的荧光强度的输入。在荧光强度杂交量转换公式计算部25中，荧光强度 用于一次确定荧光强度与对应于其的杂交量的关系的杂交量转换公式是根据存储在光点位置混合比存储部分26中的每个斑点中的探针和虚拟探针的比率和图像 从图像处理部分24提供的每个点。本发明可以适用于用于处理生物数据的该生物数据处理器。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006300799A

公开(公告)日：2006-11-02

申请号：JP2005124571

申请日：2005-04-22

Applicant: Sony Corp ,  ソニー株式会社

Inventor： OTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/64 ,  C12M1/00 ,  C12N15/09 ,  G01N21/78 ,  G01N33/53 ,  G01N33/566

CPC classification number: G01N21/6452 ,  C12Q1/6816 ,  G01N21/253 ,  C12Q2565/501

Abstract: PROBLEM TO BE SOLVED: To accurately measure the hybridization of a DNA chip at a low cost without complicating a constitution. SOLUTION: A debris 463 having the area in a spot boundary 461 is set to a spot inner region 522 and the region in the spot boundary 461 excepting the debris 463 is set to a spot inner region 521. A flag (f) is set to the respective spot inner regions 521 and 522 on the basis of its reliability. The data in the spot inner region low in reliability is not utilized. This biological data processor can be adapted to an apparatus for measuring the fluorescence intensity of the DNA chip. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：以低成本准确地测量DNA芯片的杂交而不会使构造复杂化。 解决方案：将具有点边界461中的区域的碎屑463设置到点内部区域522，并且除了碎片463之外的斑点边界461中的区域被设置为点内部区域521.标记（f） 基于其可靠性设定在各个点内部区域521和522。 不利用可靠性低的点内区域的数据。 该生物数据处理器可以适用于测量DNA芯片的荧光强度的装置。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006094707A

公开(公告)日：2006-04-13

申请号：JP2004281000

申请日：2004-09-28

Applicant: Sony Corp ,  ソニー株式会社

Inventor： MAMINE TAKAYOSHI ,  TSUJIMOTO ATSUMI ,  YAMAMOTO TAKUO

IPC: C12Q1/68 ,  C12M1/00 ,  C12N15/09 ,  G01N5/02

CPC classification number: C12Q1/6872 ,  C12Q2565/607 ,  C12Q2537/149 ,  C12Q2521/319

Abstract: PROBLEM TO BE SOLVED: To accurately and rapidly determine a base sequence of a nucleic acid molecule by measuring mass change of the nucleic acid molecule before or after cleavage treatment. SOLUTION: The prevent invention provides a method for determining a base sequence comprising at least carrying out a first step for cleaving a target nucleic molecule N while controlling a cleaving part (e.g. decomposition part by enzyme), a second step for measuring mass change of mass difference information of a nucleic acid molecule (N 1 and N 2 ) after the first step to the mass of the target nucleic acid molecule N and a third step for obtaining base information of cleaved nucleic acid molecules (n 1 and n 2 ) based on the measured data. The present invention further provides an apparatus, etc., suitable for the method. COPYRIGHT: (C)2006,JPO&NCIPI

Abstract translation: 待解决的问题：通过测量裂解处理之前或之后的核酸分子的质量变化，准确且快速地确定核酸分子的碱基序列。 解决方案：本发明提供了一种确定碱基序列的方法，包括至少在控制切割部分（例如酶的分解部分）的同时进行用于切割靶核酸分子N的第一步骤，第二步是测量质量 将第一步后的核酸分子（N 1 和N 2 ）的质量差异信息改变为靶核酸分子N的质量，第三步 基于测量数据获得切割的核酸分子的基本信息（n 1 和n 2 ）。 本发明还提供一种适用于该方法的装置等。 版权所有（C）2006，JPO＆NCIPI

公开(公告)号：JP2006325409A

公开(公告)日：2006-12-07

申请号：JP2005149325

申请日：2005-05-23

Applicant: SONY CORP

Inventor： SHU SATOSHI ,  KISHII NORIYUKI ,  TSUJIMOTO ATSUMI ,  MAMINE TAKAYOSHI

IPC: C12N15/09

Abstract: PROBLEM TO BE SOLVED: To provide a technology for purifying a poly(A)RNA having a high purity efficiently from a specimen containing nucleic acids such as a cell, etc., and a method for preparing the poly(A)RNA based on the effect of an electric field impression on a polyadenine molecule. SOLUTION: This method is to change the dipole moment and orientation of the polyadenine molecules by impressing the electric field in a reaction field. Thereby, by impressing the electric field in the reaction field, it is possible to accelerate the complement fixation of a dT chain with the poly(A) chain of the poly(A)RNA, or accelerate the addition and/or elongation of the poly(A) chain to the 3'terminal site of the RNA. COPYRIGHT: (C)2007,JPO&INPIT

公开(公告)号：JP2006300796A

公开(公告)日：2006-11-02

申请号：JP2005124563

申请日：2005-04-22

Applicant: Sony Corp ,  ソニー株式会社

Inventor： OTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/64 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/483 ,  G01N33/566

Abstract: PROBLEM TO BE SOLVED: To estimate the unknown intensity of exciting light on the basis of developed profile image data.
SOLUTION: The hybridized amount in the fluorescence intensity pf
w (pj) of a first spot of first developed profile image data is equal to the hybridized amount in the fluorescence intensity pf
s (pj) of a second spot in second developed profile image data (a value shown by β in Fig.). The hybridized amount of the fluorescence intensity pf
w (pi) of a second spot of the first developed profile image data is equal to the hybridized amount of the fluorescence intensity pf
s (pi) of the second spot of the second developed profile image data (a value shown by α in Fig.). In all spots of the first developed profile image data, a hybridized amount can be calculated using a conversion formula hybridize
w (pf) and, in all spots of the second developed profile image data, a hybridized amount can be calculated using a conversion formula hybridize
s (pf).
COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：基于开发的轮廓图像数据来估计激发光的未知强度。 解决方案：第一显影图像数据的第一个斑点的荧光强度pf w （pj）的杂交量等于荧光强度pf < / SB>（pj）（第二显影剖面图像数据中的β表示的值）。 第一显影分布图像数据的第二个斑点的荧光强度pf w /（>）的杂交量等于荧光强度pf s / pi）第二显影图像数据的第二个点（图中由α表示的值）。 在第一显影图像数据的所有斑点中，可以使用转化公式杂交（pf）来计算杂交量，并且在第二显影图像数据的所有点中，杂交量可以 使用转化公式来计算，以便混合 s （pf）。 版权所有（C）2007，JPO＆INPIT

公开(公告)号：JP2006300795A

公开(公告)日：2006-11-02

申请号：JP2005124562

申请日：2005-04-22

Applicant: Sony Corp ,  ソニー株式会社

Inventor： OTO YASUNORI ,  TSUJIMOTO ATSUMI

IPC: G01N21/78 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/64 ,  G01N33/53 ,  G01N33/543 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To set the intensity of exciting light used for measuring the hybridized state produced in a reaction region without relying on user's input. SOLUTION: Scanning for measuring the hybridized amount produced in each of the spots 12 of a DNA chip 11 is performed so as to be separated into prescanning and main scanning. The prescanning is scanning of low precision for detecting the fluorescence intensity (hybridized amount) of each of the spots 12 necessary for determining the intensity of exciting light used in the main scanning and the main scanning is scanning of high precision performed using the exciting light of intensity determined from the result of the prescanning. In the main scanning, for example, exciting light of intensity different at every spot of a measuring target is used. This biological data measuring instrument can be adapted to an apparatus for measuring the fluorescence intensity of the DNA chip. COPYRIGHT: (C)2007,JPO&INPIT

Abstract translation: 要解决的问题：设置用于测量在反应区域中产生的杂化状态的激发光的强度，而不依赖于用户的输入。 解决方案：进行用于测量在DNA芯片11的每个点12中产生的杂交量的扫描，以分离为预扫描和主扫描。 预扫描是扫描低精度以检测确定主扫描中使用的激发光的强度所需的每个斑点12的荧光强度（杂化量），并且主扫描是使用激光的激光进行的高精度扫描 根据预扫描的结果确定强度。 在主扫描中，例如使用在测定对象的各个点处的强度不同的激发光。 该生物数据测量仪器可适用于测量DNA芯片的荧光强度的装置。 版权所有（C）2007，JPO＆INPIT