公开(公告)号：WO2007141811A2

公开(公告)日：2007-12-13

申请号：PCT/IT2006000420

申请日：2006-06-06

Applicant: ST MICROELECTRONICS SRL ,  FROSINI ANDREA ,  FISCHETTI ALESSANDRA

Inventor： FROSINI ANDREA ,  FISCHETTI ALESSANDRA

CPC classification number: C12Q1/6837 ,  C12Q1/6825 ,  C12Q1/6834

Abstract: A process for preparing a semiconductor substrate for biological analysis in an integrated device, the biological analysis comprising the steps of amplifying DNA and detecting amplified DNA in the same chamber, comprises the steps of a) forming a silicon dioxide surface on said semiconductor substrate b) treating said silicon dioxide surface with a silane; c) forming a silanized surface; d) grafting nucleic acid probes; e) treating said silanized surface with a deactivating agent and f) forming a deactivated substrate sequentially. Further the process can include the step of cleaning the silicon dioxide substrate before the step of treating said silicon dioxide surface with a silane and the step of reacting the terminal group of the silane with a cross-linker or alternatively the step of reacting the derivatized nucleic acid probes with a cross-linker, before the grafting step.

Abstract translation: 一种用于制备用于集成器件中的生物分析的半导体衬底的方法，所述生物分析包括扩增DNA并在同一室中检测扩增的DNA的步骤，包括以下步骤：a）在所述半导体衬底上形成二氧化硅表面b） 用硅烷处理所述二氧化硅表面; c）形成硅烷化表面; d）接枝核酸探针; e）用去活化剂处理所述硅烷化表面，和f）顺序形成失活的底物。 此外，该方法可以包括在用硅烷处理所述二氧化硅表面的步骤之前清洁二氧化硅底物的步骤，以及使硅烷的末端基团与交联剂反应的步骤，或者可选地，使衍生的核酸 在接枝步骤之前用交联剂的酸性探针。

公开(公告)号：DE602004030678D1

公开(公告)日：2011-02-03

申请号：DE602004030678

申请日：2004-07-19

Applicant: ST MICROELECTRONICS SRL

Inventor： PALMIERI MICHELE ,  FISCHETTI ALESSANDRA

IPC: B01L3/00 ,  B01J19/00 ,  G01N27/447 ,  G01N33/543