Abstract:
0 An improved hydrolysate can be prepared from a water-soluble protein wherein the protein has been heated under alkaline conditions for a period of time insufficient to gel the protein followed by cooling the solution to below 30°C. A process for enzymatically hydrolyzing a proteinaceous material to provide an improved hydrolysate comprises (a) adjusting the pH of an aqueous solution of a proteinaceous material to a pH within the range of from 7.5 to 10 and at least 0.5 pH units above the native pH of the solution of proteinaceous material, the solution having a temperature of less than 30°C., the total dissolved protein content being within the range of from 0.5% to 20% by weight when determined at said pH; (b) heating the alkaline solution of step a) to an elevated temperature within the range of from 50°C. and 150°C. at a rate insufficient to cause gelation of the solution; (c) cooling the heated solution to a temperature within the range of from 30°C. to 2°C. within 1 hour after the solution reaches its maximum temperature level, the cooling being conducted at a rate sufficient to prevent gelation of the protein containing solution; and (d) enzymatically hydrolyzing the protein in the so treated solution.
Abstract:
A combination of fungal protease and pancreatin is used in enzymatically hydrolyzing protein. The combination of these enzymes in hydrolyzing a proteinaceous material provides good yield at short reaction time with limited organoleptic problems.
Abstract:
A combination of fungal protease and pancreatin is used in enzymatically hydrolyzing protein. The combination of these enzymes in hydrolyzing a proteinaceous material provides good yield at short reaction time with limited organoleptic problems.
Abstract:
0 An improved hydrolysate can be prepared from a water-soluble protein wherein the protein has been heated under alkaline conditions for a period of time insufficient to gel the protein followed by cooling the solution to below 30°C. A process for enzymatically hydrolyzing a proteinaceous material to provide an improved hydrolysate comprises
(a) adjusting the pH of an aqueous solution of a proteinaceous material to a pH within the range of from 7.5 to 10 and at least 0.5 pH units above the native pH of the solution of proteinaceous material, the solution having a temperature of less than 30°C., the total dissolved protein content being within the range of from 0.5% to 20% by weight when determined at said pH; (b) heating the alkaline solution of step a) to an elevated temperature within the range of from 50°C. and 150°C. at a rate insufficient to cause gelation of the solution; (c) cooling the heated solution to a temperature within the range of from 30°C. to 2°C. within 1 hour after the solution reaches its maximum temperature level, the cooling being conducted at a rate sufficient to prevent gelation of the protein containing solution; and (d) enzymatically hydrolyzing the protein in the so treated solution.
Abstract:
The thermogelation temperature of egg albumen can be decreased by cooling an alkaline solution of egg albumen heated to a temperature within the range of from 60°C. and the gelation temperature of the egg albumen, the solution having a pH within the range of from 8 and 10 and an egg albumen content in said solution of less than 20%, to a temperature sufficient to arrest further modification of the protein. The product is a thermogelable egg albumen having a low- eredthermogelation temperature.
Abstract:
A purified protein particularly adapted for enzymatic hydrolysis is prepared by gelling the protein followed by breaking the gel into particles and washing the particles in sufficient liquid to allow portions of the nonproteinaceous material in the gel matrix to diffuse into the liquid.