公开(公告)号：WO2014083384A1

公开(公告)日：2014-06-05

申请号：PCT/IB2012/056790

申请日：2012-11-28

Applicant: STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK ,  FIERS, Martijn ,  BOUTILIER, Kimberly

Inventor： FIERS, Martijn ,  BOUTILIER, Kimberly

IPC: C07C311/21 ,  A01H4/00 ,  A01N39/04 ,  A01N41/06 ,  A01N43/38 ,  A01N43/40 ,  A01N43/84 ,  A01P21/00 ,  C07C311/29 ,  C07C311/44 ,  C07D295/26 ,  C12N5/04

CPC classification number: C07C311/21 ,  A01H4/001 ,  A01H4/005 ,  A01N41/06 ,  A01N43/42 ,  C07C311/29 ,  C07C311/44 ,  C07D209/08 ,  C07D215/58 ,  C07D295/26 ,  A01N37/10 ,  A01N37/40 ,  A01N39/04 ,  A01N43/38 ,  A01N43/40 ,  A01N2300/00

Abstract: Benzenesulfonamide compounds potentiate 2,4-D induced embryogenesis in plants. In particular, 4-chloro-N-methyl-N-(2-methylphenyl) benzenesulfonamide and analogs induce somatic embryogenesis in plants. Methods of inducing somatic embryogenesis comprise exposing selected plant tissues, e.g. seed embryos, to auxins, e.g. 2.4-D and the benzenesulfonamide compounds. Compounds can be prepared by reacting sulfonyl chloride, an amine and pyridine in CH 2 CI 2 . Crude product is suspended in ethyl acetate and washed in sodium and potassium hydrogen sulphates and brine, then dried and filtered.

Abstract translation: 苯磺酰胺化合物增强植物中2,4-D诱导的胚胎发生。 特别地，4-氯-N-甲基-N-（2-甲基苯基）苯磺酰胺和类似物在植物中诱导体细胞胚发生。 诱导体细胞胚发生的方法包括将所选择的植物组织，例如， 种子胚，到生长素，例如 2.4-D和苯磺酰胺化合物。 化合物可以通过使磺酰氯，胺和吡啶在CH 2 Cl 2中反应来制备。 将粗产物悬浮于乙酸乙酯中，并在硫酸钠和氢氧化钾和盐水中洗涤，然后干燥并过滤。

公开(公告)号：WO2015043621A1

公开(公告)日：2015-04-02

申请号：PCT/EP2013/069851

申请日：2013-09-24

Applicant: STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK

Inventor： BOUTILIER, Kimberly ,  ANGENENT, Gerrit Cornelis ,  SORIANO CASTAN, Mercedes ,  HUI, Li

IPC: A01H1/08 ,  A01H1/00

CPC classification number: A01H4/008 ,  A01H1/00 ,  A01H1/08 ,  A01N37/28

Abstract: A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

Abstract translation: 单倍体胚胎发生的转变由组蛋白脱乙酰酶（HDAC）的活性控制。 用HDAC抑制剂（HDACi）阻断HDAC活性。 甘蓝型油菜中的曲古抑菌素A（TSA），甘蓝型油菜，芜菁，拟南芥和辣椒辣椒配子体，导致发生胚胎发育的细胞比例大幅增加。 在欧洲油菜中，用一种特定的HDACi（SAHA）处理可以改善这些胚胎转化（即萌发）到幼苗中。 通过向培养基中加入HDACi，可以改善在应激后培养被子植物的小孢子以产生单倍体胚，单倍体植物和双倍体植物的现有方法。 有利的是，当使用HDACi时，迄今为止难以通过小孢子培养的单倍体胚胎发生的物种变得有用。 单倍体和双倍体植物在植物育种计划中具有工业应用。

公开(公告)号：WO2015044199A1

公开(公告)日：2015-04-02

申请号：PCT/EP2014/070367

申请日：2014-09-24

Applicant: STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK

Inventor： BOUTILIER, Kimberly ,  ANGENENT, Gerrit Cornelis ,  SORIANO CASTAN, Mercedes ,  HUI, Li

IPC: A01H1/08 ,  A01H1/00

CPC classification number: A01H4/008 ,  A01H1/00 ,  A01H1/08 ,  A01N37/28

Abstract: A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, B. oleracea , Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

Abstract translation: 单倍体胚胎发生的转变由组蛋白脱乙酰酶（HDAC）的活性控制。 用HDAC抑制剂（HDACi）阻断HDAC活性。 甘蓝型油菜中的曲古抑菌素A（TSA），甘蓝型油菜，芜菁，甘蓝，拟南芥和辣椒辣椒配子体，导致胚胎发生细胞的比例大幅增加。 在欧洲油菜中，用一种特定的HDACi（SAHA）处理可以改善这些胚胎转化（即萌发）到幼苗中。 通过向培养基中加入HDACi，可以改善在应激后培养被子植物的小孢子以产生单倍体胚，单倍体植物和双倍体植物的现有方法。 有利的是，当使用HDACi时，迄今为止难以通过小孢子培养的单倍体胚胎发生的物种变得有用。 单倍体和双倍体植物在植物育种计划中具有工业应用。

公开(公告)号：WO2014083383A1

公开(公告)日：2014-06-05

申请号：PCT/IB2012/056789

申请日：2012-11-28

Applicant: STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK ,  FIERS, Martijn ,  BOUTILIER, Kimberly

Inventor： FIERS, Martijn ,  BOUTILIER, Kimberly

IPC: C07D401/04 ,  A01H4/00 ,  A01N43/40 ,  A01N43/42 ,  A01P21/00 ,  C07D215/54 ,  C12N5/04

CPC classification number: A01N43/42 ,  A01H4/001 ,  A01N43/38 ,  A01N43/40 ,  C07D215/54 ,  C07D401/04

Abstract: Cyclopentyl 2,7,7-trimethyl-5-oxo-4-(4-pyridinyl)-1,4,5,6,7,8-hexahydro-3- quinolinecarboxylate and similar compounds are potentiators of auxin-induced somatic embryogenesis in plants. In particular, the inventors have discovered certain of these compounds induce somatic embryogenesis in Arabidopsis in the presence of 2,4-D. Also tested is BAY K 8644. Methods of inducing somatic embryogenesis comprise exposing selected plant tissues, e.g. seed embryos, to auxins, e.g. 2.4-D and the compounds.

Abstract translation: 环戊基2,7,7-三甲基-5-氧代-4-（4-吡啶基）-1,4,5,6,7,8-六氢-3-喹啉羧酸酯和类似化合物是生长素诱导的体细胞胚发生的增强剂 植物。 特别地，本发明人发现这些化合物中的某些化合物在2,4-D存在下诱导拟南芥中的体细胞胚发生。 还测试了BAY K 8644.诱导体细胞胚发生的方法包括将所选择的植物组织，例如， 种子胚，到生长素，例如 2.4-D和化合物。

公开(公告)号：EP2925739A1

公开(公告)日：2015-10-07

申请号：EP12889123.1

申请日：2012-11-28

Applicant: Stichting Dienst Landbouwkundig Onderzoek

Inventor： FIERS, Martijn ,  BOUTILIER, Kimberly

IPC: C07D401/04 ,  A01H4/00 ,  A01N43/40 ,  A01N43/42 ,  A01P21/00 ,  C07D215/54 ,  C12N5/04

CPC classification number: A01N43/42 ,  A01H4/001 ,  A01N43/38 ,  A01N43/40 ,  C07D215/54 ,  C07D401/04

Abstract: Cyclopentyl 2,7,7-trimethyl-5-oxo-4-(4-pyridinyl)-1,4,5,6,7,8-hexahydro-3- quinolinecarboxylate and similar compounds are potentiators of auxin-induced somatic embryogenesis in plants. In particular, the inventors have discovered certain of these compounds induce somatic embryogenesis in Arabidopsis in the presence of 2,4-D. Also tested is BAY K 8644. Methods of inducing somatic embryogenesis comprise exposing selected plant tissues, e.g. seed embryos, to auxins, e.g. 2.4-D and the compounds.

公开(公告)号：EP3048874A1

公开(公告)日：2016-08-03

申请号：EP14772335.7

申请日：2014-09-24

Applicant: Stichting Dienst Landbouwkundig Onderzoek

Inventor： BOUTILIER, Kimberly ,  ANGENENT, Gerrit Cornelis ,  SORIANO CASTAN, Mercedes ,  HUI, Li

IPC: A01H1/08 ,  A01H1/00

CPC classification number: A01H4/008 ,  A01H1/00 ,  A01H1/08 ,  A01N37/28

Abstract: A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, B. oleracea, Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

Abstract translation: 单倍体胚胎发生的转变受组蛋白脱乙酰酶（HDACs）的活性控制。 用HDAC抑制剂（HDACi）阻断HDAC活性，例如， 在欧洲油菜，芜菁，甘蓝，拟南芥和辣椒辣椒雄配子体中的曲古抑菌素A（TSA）导致经历胚发生生长的细胞比例的大幅增加。 在欧洲油菜中，用一种特定的HDACi（SAHA）处理改善了这些胚胎向幼苗的转化（即萌发）。 通过向培养基中加入HDACi可以改善在应激后培养被子植物小孢子的小孢子以产生单倍体胚，单倍体植物和双倍体植物的现有方法。 有利地，当使用HDACi时，迄今通过小孢子培养难以达到单倍体胚胎发生的物种变得有用。 单倍体和双倍体植物在植物育种程序中具有工业应用。

公开(公告)号：EP2925717A1

公开(公告)日：2015-10-07

申请号：EP12889119.9

申请日：2012-11-28

Applicant: Stichting Dienst Landbouwkundig Onderzoek

Inventor： FIERS, Martijn ,  BOUTILIER, Kimberly

IPC: C07C311/21 ,  A01H4/00 ,  A01N39/04 ,  A01N41/06 ,  A01N43/38 ,  A01N43/40 ,  A01N43/84 ,  A01P21/00 ,  C07C311/29 ,  C07C311/44 ,  C07D295/26 ,  C12N5/04

CPC classification number: C07C311/21 ,  A01H4/001 ,  A01H4/005 ,  A01N41/06 ,  A01N43/42 ,  C07C311/29 ,  C07C311/44 ,  C07D209/08 ,  C07D215/58 ,  C07D295/26 ,  A01N37/10 ,  A01N37/40 ,  A01N39/04 ,  A01N43/38 ,  A01N43/40 ,  A01N2300/00

Abstract: Benzenesulfonamide compounds potentiate 2,4-D induced embryogenesis in plants. In particular, 4-chloro-N-methyl-N-(2-methylphenyl) benzenesulfonamide and analogs induce somatic embryogenesis in plants. Methods of inducing somatic embryogenesis comprise exposing selected plant tissues, e.g. seed embryos, to auxins, e.g. 2.4-D and the benzenesulfonamide compounds. Compounds can be prepared by reacting sulfonyl chloride, an amine and pyridine in CH
2 CI
2 . Crude product is suspended in ethyl acetate and washed in sodium and potassium hydrogen sulphates and brine, then dried and filtered.