公开(公告)号：WO2015043621A1

公开(公告)日：2015-04-02

申请号：PCT/EP2013/069851

申请日：2013-09-24

Applicant: STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK

Inventor： BOUTILIER, Kimberly ,  ANGENENT, Gerrit Cornelis ,  SORIANO CASTAN, Mercedes ,  HUI, Li

IPC: A01H1/08 ,  A01H1/00

CPC classification number: A01H4/008 ,  A01H1/00 ,  A01H1/08 ,  A01N37/28

Abstract: A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

Abstract translation: 单倍体胚胎发生的转变由组蛋白脱乙酰酶（HDAC）的活性控制。 用HDAC抑制剂（HDACi）阻断HDAC活性。 甘蓝型油菜中的曲古抑菌素A（TSA），甘蓝型油菜，芜菁，拟南芥和辣椒辣椒配子体，导致发生胚胎发育的细胞比例大幅增加。 在欧洲油菜中，用一种特定的HDACi（SAHA）处理可以改善这些胚胎转化（即萌发）到幼苗中。 通过向培养基中加入HDACi，可以改善在应激后培养被子植物的小孢子以产生单倍体胚，单倍体植物和双倍体植物的现有方法。 有利的是，当使用HDACi时，迄今为止难以通过小孢子培养的单倍体胚胎发生的物种变得有用。 单倍体和双倍体植物在植物育种计划中具有工业应用。

公开(公告)号：WO2015044199A1

公开(公告)日：2015-04-02

申请号：PCT/EP2014/070367

申请日：2014-09-24

Applicant: STICHTING DIENST LANDBOUWKUNDIG ONDERZOEK

Inventor： BOUTILIER, Kimberly ,  ANGENENT, Gerrit Cornelis ,  SORIANO CASTAN, Mercedes ,  HUI, Li

IPC: A01H1/08 ,  A01H1/00

CPC classification number: A01H4/008 ,  A01H1/00 ,  A01H1/08 ,  A01N37/28

Abstract: A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, B. oleracea , Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

Abstract translation: 单倍体胚胎发生的转变由组蛋白脱乙酰酶（HDAC）的活性控制。 用HDAC抑制剂（HDACi）阻断HDAC活性。 甘蓝型油菜中的曲古抑菌素A（TSA），甘蓝型油菜，芜菁，甘蓝，拟南芥和辣椒辣椒配子体，导致胚胎发生细胞的比例大幅增加。 在欧洲油菜中，用一种特定的HDACi（SAHA）处理可以改善这些胚胎转化（即萌发）到幼苗中。 通过向培养基中加入HDACi，可以改善在应激后培养被子植物的小孢子以产生单倍体胚，单倍体植物和双倍体植物的现有方法。 有利的是，当使用HDACi时，迄今为止难以通过小孢子培养的单倍体胚胎发生的物种变得有用。 单倍体和双倍体植物在植物育种计划中具有工业应用。

公开(公告)号：EP3048874A1

公开(公告)日：2016-08-03

申请号：EP14772335.7

申请日：2014-09-24

Applicant: Stichting Dienst Landbouwkundig Onderzoek

Inventor： BOUTILIER, Kimberly ,  ANGENENT, Gerrit Cornelis ,  SORIANO CASTAN, Mercedes ,  HUI, Li

IPC: A01H1/08 ,  A01H1/00

CPC classification number: A01H4/008 ,  A01H1/00 ,  A01H1/08 ,  A01N37/28

Abstract: A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g. trichostatin A (TSA), in Brassica napus, B. rapa, B. oleracea, Arabidopsis thaliana and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e. germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

Abstract translation: 单倍体胚胎发生的转变受组蛋白脱乙酰酶（HDACs）的活性控制。 用HDAC抑制剂（HDACi）阻断HDAC活性，例如， 在欧洲油菜，芜菁，甘蓝，拟南芥和辣椒辣椒雄配子体中的曲古抑菌素A（TSA）导致经历胚发生生长的细胞比例的大幅增加。 在欧洲油菜中，用一种特定的HDACi（SAHA）处理改善了这些胚胎向幼苗的转化（即萌发）。 通过向培养基中加入HDACi可以改善在应激后培养被子植物小孢子的小孢子以产生单倍体胚，单倍体植物和双倍体植物的现有方法。 有利地，当使用HDACi时，迄今通过小孢子培养难以达到单倍体胚胎发生的物种变得有用。 单倍体和双倍体植物在植物育种程序中具有工业应用。