公开(公告)号：NL2020257B1

公开(公告)日：2019-07-15

申请号：NL2020257

申请日：2018-01-09

Applicant: STICHTING VU

Inventor： GRAEME ALAN KING ,  FEDERICA BURLA ,  ERWIN JOHANNES GERARD PETERMAN ,  GIJS JAN LODEWIJK WUITE

IPC: C12N15/63 ,  A61K31/711 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/6806

Abstract: A method for supercoiling DNA, e.g. negatively supercoiling DNA, is disclosed. In an initial state, at least part of a DNA molecule is torsionally constrained and associated with a first linking number. The at least part of the DNA molecule has a first end connected to a first body and a second end connected to a second body. The method comprises increasing a distance between the first body and the second body for inducing a torque in the at least part of the DNA molecule and for bringing the at least part of the DNA molecule from the initial state into an intermediate state. In the intermediate state the at least part of the DNA molecule is temporarily torsionally unconstrained for at least partially releasing the induced torque for changing, e.g. decreasing, the first linking number. The method further comprises decreasing the distance between the first and second body for bringing the at least part of the DNA molecule from the intermediate state into a further state in which the at least part of the DNA nmlecule is torsionally constrained and associated with a second linking number different from the first linking number.

公开(公告)号：NL2019937B1

公开(公告)日：2019-05-24

申请号：NL2019937

申请日：2017-11-17

Applicant: STICHTING VU

Inventor： GRAEME ALAN KING ,  ANDREAS SEBASTIAN BIEBRICHER ,  ERWIN JOHANNES GERARD PETERMAN ,  IDDO HELLER ,  GIJS JAN LODEWIJK WUITE

IPC: G01L1/24 ,  G01N15/14 ,  G02B21/00

Abstract: One aspect of this disclosure relates to a computer- implemented method for determining a force acting on at least 5 part of a structure, for example a biological structure, such as a DNA molecule. The method comprises controlling a light- sensitive system, e.g. of a microscope, to determine light information based on light from the structure. The light is incident on at least a part of the light sensitive system. The 10 light-sensitive system may be said to capture the light from the structure. The at least part of the structure comprises one or more optically active entities, such as DNA intercalator molecules and donor/acceptor fluorophores. At least one of (i) an optical activity of the entities and (ii) 15 a quantity of the entities depends on the force acting on the at least part of the structure. Furthermore, the light information defines a light property value associated with said at least part of the structure. The method further comprises determining the force acting on the at least part of 20 the structure on the basis of said light property value and a reference light property value.

公开(公告)号：NL2020862B1

公开(公告)日：2019-11-12

申请号：NL2020862

申请日：2018-05-02

Applicant: LUMICKS TECH B V ,  STICHTING VU

Inventor： GIJS JAN LODEWIJK WUITE ,  RAYA SORKIN ,  DOUWE KAMSMA ,  GERRIT SITTERS ,  ERWIN JOHANNES GERARD PETERMAN ,  GIULIA BERGAMASCHI ,  MATTIJS DE GROOT

IPC: B01L3/00 ,  G01N29/06

Abstract: A method for probing mechanical properties of cellular bodies is described wherein the method includes: providing a 5 plurality of particles in a fluid medium contained in a holding space of a sample holder, each of the plurality of particles being attached to a cellular body and each cellular body being positioned on a surface of the holding space; generating a resonant bulk acoustic wave in the holding space, the resonant 10 bulk acoustic wave exerting an acoustic force on each of the plurality of particles in a direction away from the surface of the holding space or in a direction towards the surface of the holding space, each of the plurality of particles having an acoustic contrast factor and a size, the acoustic contrast 15 factor and the size being selected such that the force exerted on a particle is larger than the force exerted on the cellular body to which the particle is attached; and, for at least part of the particles attached to the cellular bodies, measuring a displacement of a particle in response to the exertion of the 20 force on the particle, the measured displacement being associated with at least one mechanical property of the cellular body attached to the particle. Fig. ZB

公开(公告)号：NL2020377B1

公开(公告)日：2019-08-14

申请号：NL2020377

申请日：2018-02-05

Applicant: LUMICKS TECH B V ,  STICHTING VU

Inventor： IDDO HELLER ,  ANNA ELISABETH CHRISTINA MEIJERING ,  ANDREAS SEBASTIAN BIEBRICHER ,  GIJS JAN LODEWIJK WUITE ,  ERWIN JOHANNES GERARD PETERMAN

IPC: G01N33/58

Abstract: One aspect of this disclosure relates to a method for determining a presence of at least one guest structure at a host structure. The method comprises a light—sensitive system receiving light from the host structure. The host structure hosts one or more optically active entities at at least one part of the host structure. Herein, the at least one part does not host the at least one guest structure. Furthermore, the optically active entities cause light emission from said at least one part. The method also comprises the light—sensitive system outputting a signal based on the received light. The method further comprises determining a light value based on the output signal. The light value indicates an amount of light from the host structure being incident on the light— sensitive system. The method also comprises determining on the basis of the light value at least one of a quantity and a position of the at least one guest structure at the host structure.

公开(公告)号：NL2014262B1

公开(公告)日：2016-10-13

申请号：NL2014262

申请日：2015-02-09

Applicant: STICHTING VU

Inventor： ANDREAS SEBASTIAN BIEBRICHER ,  ANDREA CANDELLI ,  ERWIN JOHANNES GERARD PETERMAN ,  IDDO HELLER ,  NIELS LAURENS ,  GIJS JAN LODEWIJK WUITE

IPC: B01L3/00 ,  G02B21/32

Abstract: The present disclosure relates to an apparatus (lOO) and method for controlling a plurality of simultaneously active optical traps (0Tl,OT2,0T3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (0Tl,OT2,0T3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.