公开(公告)号：NL2020257B1

公开(公告)日：2019-07-15

申请号：NL2020257

申请日：2018-01-09

Applicant: STICHTING VU

Inventor： GRAEME ALAN KING ,  FEDERICA BURLA ,  ERWIN JOHANNES GERARD PETERMAN ,  GIJS JAN LODEWIJK WUITE

IPC: C12N15/63 ,  A61K31/711 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/6806

Abstract: A method for supercoiling DNA, e.g. negatively supercoiling DNA, is disclosed. In an initial state, at least part of a DNA molecule is torsionally constrained and associated with a first linking number. The at least part of the DNA molecule has a first end connected to a first body and a second end connected to a second body. The method comprises increasing a distance between the first body and the second body for inducing a torque in the at least part of the DNA molecule and for bringing the at least part of the DNA molecule from the initial state into an intermediate state. In the intermediate state the at least part of the DNA molecule is temporarily torsionally unconstrained for at least partially releasing the induced torque for changing, e.g. decreasing, the first linking number. The method further comprises decreasing the distance between the first and second body for bringing the at least part of the DNA molecule from the intermediate state into a further state in which the at least part of the DNA nmlecule is torsionally constrained and associated with a second linking number different from the first linking number.