公开(公告)号：NL2020257B1

公开(公告)日：2019-07-15

申请号：NL2020257

申请日：2018-01-09

Applicant: STICHTING VU

Inventor： GRAEME ALAN KING ,  FEDERICA BURLA ,  ERWIN JOHANNES GERARD PETERMAN ,  GIJS JAN LODEWIJK WUITE

IPC: C12N15/63 ,  A61K31/711 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/6806

Abstract: A method for supercoiling DNA, e.g. negatively supercoiling DNA, is disclosed. In an initial state, at least part of a DNA molecule is torsionally constrained and associated with a first linking number. The at least part of the DNA molecule has a first end connected to a first body and a second end connected to a second body. The method comprises increasing a distance between the first body and the second body for inducing a torque in the at least part of the DNA molecule and for bringing the at least part of the DNA molecule from the initial state into an intermediate state. In the intermediate state the at least part of the DNA molecule is temporarily torsionally unconstrained for at least partially releasing the induced torque for changing, e.g. decreasing, the first linking number. The method further comprises decreasing the distance between the first and second body for bringing the at least part of the DNA molecule from the intermediate state into a further state in which the at least part of the DNA nmlecule is torsionally constrained and associated with a second linking number different from the first linking number.

公开(公告)号：NL2019937B1

公开(公告)日：2019-05-24

申请号：NL2019937

申请日：2017-11-17

Applicant: STICHTING VU

Inventor： GRAEME ALAN KING ,  ANDREAS SEBASTIAN BIEBRICHER ,  ERWIN JOHANNES GERARD PETERMAN ,  IDDO HELLER ,  GIJS JAN LODEWIJK WUITE

IPC: G01L1/24 ,  G01N15/14 ,  G02B21/00

Abstract: One aspect of this disclosure relates to a computer- implemented method for determining a force acting on at least 5 part of a structure, for example a biological structure, such as a DNA molecule. The method comprises controlling a light- sensitive system, e.g. of a microscope, to determine light information based on light from the structure. The light is incident on at least a part of the light sensitive system. The 10 light-sensitive system may be said to capture the light from the structure. The at least part of the structure comprises one or more optically active entities, such as DNA intercalator molecules and donor/acceptor fluorophores. At least one of (i) an optical activity of the entities and (ii) 15 a quantity of the entities depends on the force acting on the at least part of the structure. Furthermore, the light information defines a light property value associated with said at least part of the structure. The method further comprises determining the force acting on the at least part of 20 the structure on the basis of said light property value and a reference light property value.