公开(公告)号：US09862941B2

公开(公告)日：2018-01-09

申请号：US15288969

申请日：2016-10-07

Applicant: The Regents of the University of California ,  Pioneer Hi-Bred International, Inc.

Inventor： Abraham P. Lee ,  Do-Hyun Lee ,  H. Kumar Wickramasinghe ,  Yinglei Tao ,  Xuan Li ,  Yue Yun

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00

CPC classification number: C12N15/1006 ,  B01L3/00 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2400/086 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2565/107

Abstract: Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

公开(公告)号：US09658162B2

公开(公告)日：2017-05-23

申请号：US14642655

申请日：2015-03-09

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： H. Kumar Wickramasinghe

IPC: G01N21/65 ,  G01J3/44 ,  G01J3/45 ,  G01Q30/02

CPC classification number: G01N21/65 ,  G01J3/0224 ,  G01J3/44 ,  G01J3/45 ,  G01J3/453 ,  G01J9/0215 ,  G01J2009/0261 ,  G01N2021/655 ,  G01Q30/02

Abstract: An apparatus and method for measuring amplitude and/or phase of a molecular vibration uses a polarization modulated pump beam and a stimulating Stokes beam on a probe of a scanning probe microscope to detect a Raman scattered Stokes beam from the sample. The detected Raman scattered Stokes beam is used to derive at least one of the amplitude and the phase of the molecular vibration.

公开(公告)号：US10549277B2

公开(公告)日：2020-02-04

申请号：US15697898

申请日：2017-09-07

Applicant: The Regents of the University of California

Inventor： Abraham P. Lee ,  H. Kumar Wickramasinghe ,  Do-Hyun Lee ,  Yinglei Tao ,  Xuan Li

IPC: B01L3/00 ,  C12Q1/6886 ,  C12Q1/6806 ,  C12N15/10

Abstract: Disclosed is a lab-on-a-chip platform comprising a microfluidic device sealed with a thin membrane. An analysis probe configured to penetrate the thin membrane to access a sample trapped in the microfluidic device.

公开(公告)号：US10526595B2

公开(公告)日：2020-01-07

申请号：US15950982

申请日：2018-04-11

Applicant: The Regents of the University of California ,  Pioneer Hi-Bred International, Inc.

Inventor： Abraham P. Lee ,  Do-Hyun Lee ,  H. Kumar Wickramasinghe ,  Yinglei Tao ,  Xuan Li ,  Yue Yun

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6844 ,  C12Q1/6806

Abstract: Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

公开(公告)号：US20170107507A1

公开(公告)日：2017-04-20

申请号：US15288969

申请日：2016-10-07

Applicant: The Regents of the University of California ,  Pioneer Hi-Bred International, Inc.

Inventor： Abraham P. Lee ,  Do-Hyun Lee ,  H. Kumar Wickramasinghe ,  Yinglei Tao ,  Xuan Li ,  Yue Yun

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1006 ,  B01L3/00 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2400/086 ,  C12N15/101 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2565/107

Abstract: Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

公开(公告)号：US20150253248A1

公开(公告)日：2015-09-10

申请号：US14642655

申请日：2015-03-09

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： H. Kumar Wickramasinghe

IPC: G01N21/65 ,  G01J3/45 ,  G01J3/44

CPC classification number: G01N21/65 ,  G01J3/0224 ,  G01J3/44 ,  G01J3/45 ,  G01J3/453 ,  G01J9/0215 ,  G01J2009/0261 ,  G01N2021/655 ,  G01Q30/02

Abstract: An apparatus and method for measuring amplitude and/or phase of a molecular vibration uses a polarization modulated pump beam and a stimulating Stokes beam on a probe of a scanning probe microscope to detect a Raman scattered Stokes beam from the sample. The detected Raman scattered Stokes beam is used to derive at least one of the amplitude and the phase of the molecular vibration.

Abstract translation: 用于测量分子振动的幅度和/或相位的装置和方法使用偏振调制泵浦光束和刺激斯托克斯光束在扫描探针显微镜的探针上以检测来自样品的拉曼散射斯托克斯光束。 检测到的拉曼散射斯托克斯光束用于推导分子振动的振幅和相位中的至少一个。

公开(公告)号：US20180230453A1

公开(公告)日：2018-08-16

申请号：US15950982

申请日：2018-04-11

Applicant: The Regents of the University of California ,  Pioneer Hi-Bred International, Inc.

Inventor： Abraham P. Lee ,  Do-Hyun Lee ,  H. Kumar Wickramasinghe ,  Yinglei Tao ,  Xuan Li ,  Yue Yun

IPC: C12N15/10 ,  C12Q1/6806 ,  B01L3/00 ,  C12Q1/6844

CPC classification number: C12N15/1006 ,  B01L3/00 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0681 ,  B01L2400/086 ,  C12N15/101 ,  C12N15/1017 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2565/101 ,  C12Q2565/107 ,  C40B40/08

Abstract: Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

公开(公告)号：US20180078940A1

公开(公告)日：2018-03-22

申请号：US15697898

申请日：2017-09-07

Applicant: The Regents of the University of California

Inventor： Abraham P. Lee ,  H. Kumar Wickramasinghe ,  Do-Hyun Lee ,  Yinglei Tao ,  Xuan Li

IPC: B01L3/00 ,  C12Q1/68 ,  C12N15/10

Abstract: Disclosed is a lab-on-a-chip platform comprising a microfluidic device sealed with a thin membrane. An analysis probe configured to penetrate the thin membrane to access a sample trapped in the microfluidic device.