公开(公告)号：JPH02249479A

公开(公告)日：1990-10-05

申请号：JP7143689

申请日：1989-03-23

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  AIBA SHUICHI ,  GONDA KINJI ,  KAJIO KAZUTO

IPC: G01N35/08 ,  C12M1/34

Abstract: PURPOSE:To enable measurement of an extracellular ATP concentration, an extracellular and intracellular ATP concentration and blank value switching in order in a short time by operating many pumps and injectors corresponding to a measuring object. CONSTITUTION:For measurement of an extracellular ATP (adenosine triphosphate) concentration, the injector 1 is rotated in order to change the passage to the carrier liquid (C1) after supplying a cell suspension (S) to the injector 1 and the injector 2 is simultaneously changed in order to send a luminous agent (E) sandwiching the luminous agent (E) with carrier liquids (C2) after supplying the luminous agent (E) to the injector 2. For measurement of an intracellular ATP concentration, an ATP-extracted solution (R) is injected into the sample passage 3 from the supply passage 10 before a cell suspension (S) injected between the carrier liquid (C1) by the injector 1 reaches a mixer 6. Blank value is measured by supplying only the carrier liquid (C1) and the carrier liquid (C2).