公开(公告)号：JPH03123481A

公开(公告)日：1991-05-27

申请号：JP26170989

申请日：1989-10-06

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  GONDA KINJI ,  KAJIWARA KAZUTO

IPC: C12M1/34 ,  C12Q1/06 ,  C12Q1/66

Abstract: PURPOSE:To determine the cell concentration and cell activity in a cell suspension liquid at the same time by placing a chemiluminescence detector in a detection channel and an absorbance detector in a sample supplying channel, etc., mixing a sample supplied through a sample channel and a reagent supplied through a reagent channel in a mixer and transferring the mixture through the detection channel. CONSTITUTION:A sample liquid S containing suspended cells in injected into a carrier liquid C1 and an ATP extraction liquid R is injected into the carrier liquid before a mixer 11. A luminescent reagent E containing luciferin and luciferase is injected into a carrier liquid C2 and the sample liquid is mixed to the reagent liquid with a mixer 12. A sample channel 2 is joined to a reagent channel 4 and the mixture is passed through the mixer 13 to a chemiluminescence detector 6 and an absorbance detector 7 to determine the ATP concentration and turbidity. The ATP concentration in the cell and the concentration outside of the cell can be separately determined by injecting the ATP extraction liquid R and without using the injection of the liquid.

公开(公告)号：JPH02249970A

公开(公告)日：1990-10-05

申请号：JP7143589

申请日：1989-03-23

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  AIBA SHUICHI ,  GONDA KINJI ,  KAJIWARA KAZUTO

IPC: G01N35/00 ,  C12M1/34 ,  G01N35/08

Abstract: PURPOSE:To prolong the life of a reagent and calibrating liquid by providing a reagent vessel connected to a detector in a thermostatic chamber which maintains the vessel at a room temp. or below. CONSTITUTION:After a sample liquid S is supplied by a pump 7 to an injector 10, the injector 10 is rotated to switch the flow passage to the flow passage of a carrier liquid C1 and the sample liquid S cut off in such a manner is inserted by the carrier liquid C1 fed by a pump 6 and is mixed 12. The flow passage of the sample liquid S is connected to the flow passage from a calibrat ing liquid vessel 1 by a 3-way valve 5 just before the pump 7. The calibrating liquid can be supplied to the ejector 10 in place of the sample liquid S by switching the 3-way valve 5 at the time of calibration. On the other hand, the reagent is supplied from the reagent vessel 2 by a pump 9 to another injector 11 and the reagent cut off by a similarly switching the injector 11 is inserted by the carrier liquid C2 and is mixed 13 by a pump 8. The liquid mixture from the mixers 12, 13 is further mixed 14 and is passed through a detector 15, from which the liquid mixture is discharged. This liquid mixture is processed 16 as an electrical signal and the concn. thereof is determined.

公开(公告)号：JPH02249479A

公开(公告)日：1990-10-05

申请号：JP7143689

申请日：1989-03-23

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  AIBA SHUICHI ,  GONDA KINJI ,  KAJIO KAZUTO

IPC: G01N35/08 ,  C12M1/34

Abstract: PURPOSE:To enable measurement of an extracellular ATP concentration, an extracellular and intracellular ATP concentration and blank value switching in order in a short time by operating many pumps and injectors corresponding to a measuring object. CONSTITUTION:For measurement of an extracellular ATP (adenosine triphosphate) concentration, the injector 1 is rotated in order to change the passage to the carrier liquid (C1) after supplying a cell suspension (S) to the injector 1 and the injector 2 is simultaneously changed in order to send a luminous agent (E) sandwiching the luminous agent (E) with carrier liquids (C2) after supplying the luminous agent (E) to the injector 2. For measurement of an intracellular ATP concentration, an ATP-extracted solution (R) is injected into the sample passage 3 from the supply passage 10 before a cell suspension (S) injected between the carrier liquid (C1) by the injector 1 reaches a mixer 6. Blank value is measured by supplying only the carrier liquid (C1) and the carrier liquid (C2).