公开(公告)号：JPH02249970A

公开(公告)日：1990-10-05

申请号：JP7143589

申请日：1989-03-23

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  AIBA SHUICHI ,  GONDA KINJI ,  KAJIWARA KAZUTO

IPC: G01N35/00 ,  C12M1/34 ,  G01N35/08

Abstract: PURPOSE:To prolong the life of a reagent and calibrating liquid by providing a reagent vessel connected to a detector in a thermostatic chamber which maintains the vessel at a room temp. or below. CONSTITUTION:After a sample liquid S is supplied by a pump 7 to an injector 10, the injector 10 is rotated to switch the flow passage to the flow passage of a carrier liquid C1 and the sample liquid S cut off in such a manner is inserted by the carrier liquid C1 fed by a pump 6 and is mixed 12. The flow passage of the sample liquid S is connected to the flow passage from a calibrat ing liquid vessel 1 by a 3-way valve 5 just before the pump 7. The calibrating liquid can be supplied to the ejector 10 in place of the sample liquid S by switching the 3-way valve 5 at the time of calibration. On the other hand, the reagent is supplied from the reagent vessel 2 by a pump 9 to another injector 11 and the reagent cut off by a similarly switching the injector 11 is inserted by the carrier liquid C2 and is mixed 13 by a pump 8. The liquid mixture from the mixers 12, 13 is further mixed 14 and is passed through a detector 15, from which the liquid mixture is discharged. This liquid mixture is processed 16 as an electrical signal and the concn. thereof is determined.

公开(公告)号：JPH03123481A

公开(公告)日：1991-05-27

申请号：JP26170989

申请日：1989-10-06

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  MOTOHASHI RYOICHI ,  GONDA KINJI ,  KAJIWARA KAZUTO

IPC: C12M1/34 ,  C12Q1/06 ,  C12Q1/66

Abstract: PURPOSE:To determine the cell concentration and cell activity in a cell suspension liquid at the same time by placing a chemiluminescence detector in a detection channel and an absorbance detector in a sample supplying channel, etc., mixing a sample supplied through a sample channel and a reagent supplied through a reagent channel in a mixer and transferring the mixture through the detection channel. CONSTITUTION:A sample liquid S containing suspended cells in injected into a carrier liquid C1 and an ATP extraction liquid R is injected into the carrier liquid before a mixer 11. A luminescent reagent E containing luciferin and luciferase is injected into a carrier liquid C2 and the sample liquid is mixed to the reagent liquid with a mixer 12. A sample channel 2 is joined to a reagent channel 4 and the mixture is passed through the mixer 13 to a chemiluminescence detector 6 and an absorbance detector 7 to determine the ATP concentration and turbidity. The ATP concentration in the cell and the concentration outside of the cell can be separately determined by injecting the ATP extraction liquid R and without using the injection of the liquid.