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    PRODUCTION OF VON WILEBRAND FACTOR
    3.
    发明专利
    PRODUCTION OF VON WILEBRAND FACTOR 失效

    公开(公告)号：JPH08140692A

    公开(公告)日：1996-06-04

    申请号：JP29166394

    申请日：1994-11-25

    Applicant: TORAY INDUSTRIES

    Inventor： SANO EMIKO ,  HOKARI NAOTO ,  IIZUKA MASAHIKO

    IPC: C12M3/00 ,  A61K38/43 ,  A61P7/04 ,  C07K14/745 ,  C12P21/00 ,  C12R1/91

    Abstract: PURPOSE: To efficiently obtain the subject protein for treatment, etc., of von Wilebrand disease which is a congenital hemorrhagic disease in large amounts by culturing a vascular endothelial cell attached onto a microcarrier while adding shearing stress in a specific range. CONSTITUTION: This von Wilebrand factor useful for treatment, etc., of von Wilebrand disease which is a congenital hemorrhagic disease is efficiently obtained by culturing a vascular endothelial cell separated by from human umbilical vein an oxygen perfusion method in a cultured medium to which cultured supernatant of a human normal diploid fibroblast and fetal calf serum are added in a flask coated with collagen, and putting the culture into a spinner culturing bottle containing a microcarrier such as gelatin beads, inoculating a vascular endothelial cell thereinto, setting the number of rotation of stirring to 50-600rpm at 37 deg.C and culturing the cell for 8 days while applying shearing stress within a range of 20-130dyne/cm . Thereby, mass production of the von Wilebrand factor by the cultured cell which has been difficult hitherto is made possible.

    PRODUCTION OF USEFUL PROTEIN
    4.
    发明专利
    PRODUCTION OF USEFUL PROTEIN 失效

    公开(公告)号：JPS6232896A

    公开(公告)日：1987-02-12

    申请号：JP17125185

    申请日：1985-08-05

    Applicant: TORAY INDUSTRIES

    Inventor： IIZUKA MASAHIKO ,  SANO EMIKO

    IPC: C12N15/09 ,  C12N5/10 ,  C12N15/00 ,  C12N15/90 ,  C12P21/00 ,  C12R1/91

    Abstract: PURPOSE:To enable the mass-production of the objective useful protein utilizing a genetic engineering, by using an animal cell containing a vector integrated with a DNA coding the useful protein and proliferating and keeping the cell on a microcarrier. CONSTITUTION:An animal cell containing a vector integrated with a DNA coding a useful protein such as human interferon beta, human interferon gamma, human interleukin 2, etc., which can be produced by genetic engineering, is proliferated and maintained on a microcarrier. The microcarrier is generally a minute particle of a polysaccaride having positively charged chemical residue, or a minute particle of crosslinked dextran coated with collagen, etc. The necessary amount of bovine fetus serum to be added to the medium for maintaining the animal cell on the microcarrier is usually smaller than that for the proliferation medium.

    WASHING METHOD OF CELL
    6.
    发明专利
    WASHING METHOD OF CELL 失效

    公开(公告)号：JPS572682A

    公开(公告)日：1982-01-08

    申请号：JP7545880

    申请日：1980-06-06

    Applicant: TORAY INDUSTRIES

    Inventor： KUBOTA HIDEMASA ,  IIZUKA MASAHIKO ,  KIZAKI TAKAFUMI

    IPC: C12N5/02 ,  C12N5/00 ,  C12N5/07 ,  C12N5/071 ,  C12P21/00 ,  C12R1/91

    Abstract: PURPOSE:To prevent the peeling of cells from a microcarrier surface in washing thereof, by washing the cells grown on the microcarrier surface with an aqueous solution of a salt containing a nonproteinic water-soluble high polymeric substance. CONSTITUTION:Cells grown on a microcarrier surface are washed with an aqueous solution of a salt adjusted to a suitable osmotic pressure. In the process, a nonproteinic water-soluble high polymeric substance, e.g. dextran, methyl cellulose or polyvinyl pyrrolidone, in an amount in the range of 0.0001-1wt%, preferably 0.001-0.1wt%, is added to the aqueous solution.

    CELL CULTURING DEVICE
    7.
    发明专利
    CELL CULTURING DEVICE 失效

    公开(公告)号：JPS5299284A

    公开(公告)日：1977-08-19

    申请号：JP1395676

    申请日：1976-02-13

    Applicant: TORAY INDUSTRIES

    Inventor： IIZUKA MASAHIKO ,  NAKAJIYOU YOSHIHIRO

    IPC: C12M3/04 ,  C12P21/00

    Abstract: PURPOSE:To enable the mass-culturing of cells for the porduction of virus vaccine or interferon by closely stacking two or more culturing elements, each composed of a cell-attaching plate, its supporting frame and a hole connecting the inner and outer sides of the frame.

    METHOD OF INTERFERON PRODUCTION
    9.
    发明专利
    METHOD OF INTERFERON PRODUCTION 失效

    公开(公告)号：JPS56167624A

    公开(公告)日：1981-12-23

    申请号：JP7086280

    申请日：1980-05-29

    Applicant: TORAY INDUSTRIES

    Inventor： IIZUKA MASAHIKO ,  KUBOTA HIDEMASA ,  SANO EMIKO

    IPC: C12P21/00 ,  C07K14/555 ,  C12N5/00 ,  C12R1/91

    Abstract: PURPOSE:The animal cells that are grown on a microcarrier having chemical groups charged positively are treated with a specific solution to produce high units of interferone used as antiviral or antitumor. CONSTITUTION:The animal cells such as human normal diloid cells are grown on a microcarrier having chemical groups charged positively such as a crosslinked dextran bearing diethylaminoethyl groups are treated with an interferon inducer such as an RNA-dupllex, e.g., poly (I) or poly (C). At this time, the microcarrier on which the cells stick and grow is treated with an aqueous solution of a water- soluble high polymer charged negatively such as carboxymethyl cellulose or dextran sulfate, before or when the interferon inducer is added, to increase the activity of the interferon inducer and to produce high-unit interferon.

    PRODUCTION OF INTERFERON
    10.
    发明专利
    PRODUCTION OF INTERFERON 失效

    公开(公告)号：JPS5495795A

    公开(公告)日：1979-07-28

    申请号：JP188778

    申请日：1978-01-13

    Applicant: TORAY INDUSTRIES

    Inventor： IIZUKA MASAHIKO ,  HAISHI YUKIE ,  KOBAYASHI SHIGEYASU

    IPC: C12P21/00 ,  A61K38/21 ,  C12P21/02 ,  C12R1/91

    Abstract: PURPOSE:To produce interferon easily and economically, by treating interferon- producing cells with an interferon-inducing agent, and culturing the treated cells in an aqueous solution of inorganic salts containing amino acids mainly composed of glutamine. CONSTITUTION:Human deploid cells are treated with an interferon-inducing agent, such as double chain RNA, etc., and cultured in an inorganic salt medium containing amino acids wherein >=50% of the amino acids is glutamine, to afford interferon. The concentration of glutamine in the medium is pref. 100-1000 mg/1, and especially, about 300 mg/1. The medium is, compared with conventional cultivation media, economical, easily prepared, sterilized and adjusted its pH, low risk of saprophyte-contamination, and easily concentrated and purified.

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