公开(公告)号：HK1164407A1

公开(公告)日：2012-09-21

申请号：HK12104955

申请日：2012-05-21

Applicant: WATERS TECHNOLOGIES CORP

Inventor： CORMIER SYLVAIN ,  ANDREWS RICHARD W ,  TACCONI ROBERT ,  DOBBS CRAIG H

IPC: F04B20060101

公开(公告)号：EP2401502A4

公开(公告)日：2016-10-19

申请号：EP10746644

申请日：2010-02-17

Applicant: WATERS TECHNOLOGIES CORP

Inventor： CORMIER SYLVAIN ,  ANDREWS RICHARD W ,  TACCONI ROBERT ,  DOBBS CRAIG H

IPC: F04B13/02 ,  F04B23/06 ,  G01N1/38 ,  G01N1/40 ,  G01N30/20

CPC classification number: F04B13/02 ,  F04B23/06 ,  G01N1/38 ,  G01N1/40 ,  G01N30/20

Abstract: Described are a method and system for diluting a sample in a liquid chromatography system. A sample flowing at a first flow rate is combined for a predetermined time with a diluent flowing at a second flow rate to generate a volume of diluted sample. The diluted sample has a dilution ratio determined according to the two flow rates. A portion of the volume is loaded into a sample loop of an injection valve and subsequently injected into a mobile phase flowing to a chromatography column. The method eliminates the need to have a technician available to perform dilutions or to transfer the sample to a remote location for dilution. Advantageously, manufacturing or processing downtime to perform the dilution is not required and the amount of sample flowing to waste is reduced.