公开(公告)号：WO2023067487A1

公开(公告)日：2023-04-27

申请号：PCT/IB2022/059978

申请日：2022-10-18

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： LAUBER, Matthew A. ,  WYNDHAM, Kevin ,  FEKETE, Szabolcs ,  YANG, Hua ,  KOZA, Stephan

IPC: G01N30/88 ,  G01N30/96 ,  G01N30/54 ,  G01N30/34

Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30°C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.

公开(公告)号：EP4419904A1

公开(公告)日：2024-08-28

申请号：EP22817804.2

申请日：2022-10-18

Applicant: Waters Technologies Corporation

Inventor： LAUBER, Matthew A. ,  WYNDHAM, Kevin ,  FEKETE, Szabolcs ,  YANG, Hua ,  KOZA, Stephan

IPC: G01N30/88 ,  G01N30/96 ,  G01N30/54 ,  G01N30/34

CPC classification number: G01N2030/882720130101 ,  G01N30/88 ,  G01N30/54 ,  G01N30/34 ,  G01N30/96