公开(公告)号：US10047392B2

公开(公告)日：2018-08-14

申请号：US15119859

申请日：2015-02-23

Applicant: Northeastern University

Inventor： Andrey Ivankin ,  Joseph Larkin ,  Robert Henley ,  Meni Wanunu

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N33/487 ,  G01N21/77

Abstract: Described herein are systems for analysis of biopolymers and complexes containing biopolymers based on optical measurement of ion flux through pores. Also described are methods of using such devices for analysis of biopolymers and complexes containing biopolymers, including methods of determining the nucleotide sequences of polynucleotides.

公开(公告)号：US20160282326A1

公开(公告)日：2016-09-29

申请号：US15037863

申请日：2014-11-25

Applicant: Northeastern University

Inventor： Pradeep Waduge ,  Joseph Larkin ,  Moneesh Upmanyu ,  Swastik Kar ,  Meni Wanunu

IPC: G01N33/487 ,  C23C16/26 ,  C12Q1/68 ,  G01N27/447

CPC classification number: G01N33/48721 ,  B82Y30/00 ,  B82Y40/00 ,  C01B32/186 ,  C12Q1/6869 ,  C23C16/0281 ,  C23C16/26 ,  C23C16/56 ,  G01N27/44704 ,  G01N27/4473 ,  G01N27/44791

Abstract: Devices contain freestanding, ultra thin (

Abstract translation: 器件包含独立，超薄（<10 nm厚）的膜和制造这些器件的方法。 使用装置的方法包含独立的超薄膜，用于确定多核苷酸的序列和用于水溶液的脱盐。 一种装置，包括：具有上表面，下表面和孔的基底，所述孔具有连接上表面和下表面并形成孔的一个或多个壁; 以及附着到所述基底的下表面并形成所述孔的底部的膜，所述膜具有小于10nm的厚度。 膜上的电导率小于1 nS /？m2。

公开(公告)号：US11041247B2

公开(公告)日：2021-06-22

申请号：US16781486

申请日：2020-02-04

Applicant: Northeastern University

Inventor： Pradeep Waduge ,  Swastik Kar ,  Meni Wanunu ,  Joseph Larkin ,  Ismail Bilgin

IPC: C25B9/23 ,  G01N33/487 ,  C01B32/186 ,  C12Q1/6869 ,  G01N27/447

Abstract: The invention provides methods for direct growth of low noise, atomically thin freestanding membranes of two-dimensional monocrystalline or polycrystalline materials, such as transition metal chalcogenides including molybdenum disulfide. The freestanding membranes are directly grown over an aperture by reacting two precursors in a chemical vapor deposition process carried out at atmospheric pressure. Membrane growth is preferentially over apertures in a thin sheet of solid state material. The resulting membranes are one or a few atomic layers thick and essentially free of defects. The membranes are useful for sequencing of biopolymers through nanopores.

公开(公告)号：US10570444B2

公开(公告)日：2020-02-25

申请号：US15572296

申请日：2016-05-11

Applicant: Northeastern University

Inventor： Meni Wanunu ,  Joseph Larkin ,  Robert Henley

IPC: C12Q1/6869 ,  G02B6/10 ,  B82Y20/00

Abstract: Devices and methods useful for sequencing and characterizing single nucleic acid molecules involve large arrays of nanopore zero-mode waveguides (NZMWs). The methods and devices are made possible by fabrication of nanoporous membranes of appropriate porosity for use in nucleotide sequencing. The fabrication methods eliminate the need for serial nanopore formation and make possible the mass production of highly efficient DNA and RNA single molecule sequencing devices.

公开(公告)号：US10550487B2

公开(公告)日：2020-02-04

申请号：US15552373

申请日：2015-11-25

Applicant: Northeastern University

Inventor： Pradeep Waduge ,  Swastik Kar ,  Meni Wanunu ,  Joseph Larkin ,  Ismail Bilgin

IPC: C25B9/10 ,  G01N33/487 ,  C01B32/186 ,  C12Q1/6869 ,  G01N27/447

Abstract: The invention provides methods for direct growth of low noise, atomically thin freestanding membranes of two-dimensional monocrystalline or polycrystalline materials, such as transition metal chalcogenides including molybdenum disulfide. The freestanding membranes are directly grown over an aperture by reacting two precursors in a chemical vapor deposition process carried out at atmospheric pressure. Membrane growth is preferentially over apertures in a thin sheet of solid state material. The resulting membranes are one or a few atomic layers thick and essentially free of defects. The membranes are useful for sequencing of biopolymers through nanopores.