Abstract:
An apparatus comprising a light source, a lens system, a reticle illuminated by the light source, a housing having a substantially dark viewing chamber and a pearl holder for holding the cultured pearl in the substantially dark viewing chamber, and a reticle device for providing a reticle image observable through the lens system. The substantially dark viewing chamber is optically connected to the light source so as to allow light from the light source to traverse an aperture in the pearl holder to illuminate the pearl. The substantially dark viewing chamber is also optically connected to the lens system. In order to provide maximum contrast between light and darkness in the substantially dark viewing chamber, the housing is arranged to prevent entry of extraneous light into the substantially dark viewing chamber. A preferred method according to the present invention includes the following steps. A pearl is placed in the substantially dark viewing chamber. The pearl is illuminated by irradiating a relatively small portion of a side of the pearl with light from the light source, so that luminescence is stimulated throughout the pearl only when the pearl is a natural pearl, and so that the thickness of the nacre coating is observable through the lens system when the pearl is a cultured pearl. The thickness of the nacre coating of the illuminated pearl is measured by comparison with the reticle image observed through the lens system.
Abstract:
A spectral cover for a reflectance photometer is disclosed in which the spectral cover is designed to cover the readhead area of the reflectance photometer and block the transmission of light in a wavelength range of at least 60 to no more than 200 nanometers centered about the detection wavelength while permitting light of other wavelengths to be transmitted. The invention permits the readhead cover to be permanently or semipermanently positioned on the reflectance photometer. Since a user is able to see through the spectral cover it is possible to position a test device correctly and quickly over the readhead of the reflectance photometer. Signal noise levels due to ambient light are substantially eliminated by the invention.
Abstract:
A reflection meter for measuring the percentage of reflected light from a surface. A light source produces light which strikes the surface. The surface reflects the light into a chamber which directs the light to a detector. The detector senses the reflected light and produces a detect signal that represents the light reflected. A microprocessor receives the detect signal and calculates a percentage of light reflected from the surface which is shown on a display. An absorbing receiver engage the surface and absorbs any light that is transmitted through the surface.
Abstract:
A reference system utilizes pulsed arc light excitation which excitation pulses are directed onto a flow cell containing a fluorescent dye. Fluorescent light emitted from the dye is guided to a photomultiplier tube which converts it to electrical pulses. A portion of each excitation light pulse is guided by a light pipe onto a PIN diode light detector which converts these light signals to electrical pulses. A LED reference light source is pulsed to generate a plurality of reference light pulses one of which occurs between each excitation pulse. A portion of each of these pulses is guided to each of the two light detectors and two more series of electrical pulses are generated. A microprocessor then reads the four electrical pulses resulting from each pair of light pulses and performs a computation on the resulting numbers which indicates the relative concentration of the target concentration being assayed. An optical system masks the excitation light pulses and the emitted light pulses to minimize the amount of scattered excitation light that gets into the emitted light optical channel and spatially integrates the images of the arc yielding the excitation light to control the stability of the image projected onto the photomultiplier tube. The light pipe and an output lens spatially integrates the image of the excitation light mask and focuses this light on the PIN diode so that the dancing image of the arc does not modulate with the variations of the PIN diode cathode and destabilize its output signal.
Abstract:
There is disclosed herein a reference system for a fluorometer designed to detect very low levels of materials tagged with fluorophores. There is also disclosed an optical system for use in such a system which improves the signal to noise ratio. The reference system utilizes pulsed arc light excitation which excitation pulses are directed onto a flow cell containing the fluorescent dye. Fluorescent light emitted from the dye is guided to a photomultiplier tube which converts it to electrical pulses. A portion of each excitation light pulse is guided by a light pipe onto a PIN diode light detector which converts these light signals to electrical pulses. A LED reference light source is pulsed to generate a plurality of reference light pulses one of which occurs between each excitation pulse. A portion of each of these pulses is guided to each of the two light detectors and two more series of electrical pulses are generated. A microprocessor then reads the four electrical pulses resulting from each pair of light pulses and performs a computation on the resulting numbers which indicates the relative concentration of the target concentration being assayed. The optical system makes the excitation light pulses and the emitted light pulses to minimize the amount of scattered excitation light that gets into the emitted light optical channel and to control the location and size of the image projected onto the photomultiplier tube to stabilize its output signal. The light pipe and an output lens spatially integrates the image of the excitation light mask and focusses this light on the PIN diode so that the dancing image of the arc does not wander off the face of the PIN diode and destabilize its output signal. The action of the flow cell fluid contents spatially intergrates the fluorescent light thereby helping to stabilize the output of the photomultiplier tube.
Abstract:
A light absorber for use in a sampling tube used with sensitive optical pollution detectors the light absorber including a central light receiving conical surface inclined by at least one annular truncated conical surface to provide at least one annular groove or valley surrounding the central cone and wherein one wall of the groove or valley is undercut to shade the base of the groove or valley from direct impinging light.
Abstract:
A flaw detector for optically transmissive surfaces having a first light collector above the surface and a second light collector below the surface. A scanning light beam is directed into the first light collector through a beam entrance aperture and only light scattered from the surface is collected. Light specularly reflected from the surface exits the collector through the beam entrance aperture. Similarly, light passing through the surface enters the second collector, but the axial beam component is dumped through an opening in the second collector, while only diffracted light is collected. Preferably, two-stage light collectors are used with the first stage admitting the beam and generating a scattered or diffracted beam component, with the second stage admitting the scattered or diffracted beam component and integrating the component over a collection surface and sampling the integrated portion at a photoelectric detector. An electrical output signal from the detector may be displayed.
Abstract:
The present invention provides a far-infrared light source capable of reducing the shift in the location irradiated with far-infrared light even when the frequency of the far-infrared light changes. A far-infrared light source according to the present invention is configured so that the variation in the emission angle of far-infrared light in a nonlinear optical crystal when the frequency of the far-infrared light changes is substantially offset by the variation in the refractive angle of the far-infrared light at the interface between the nonlinear optical crystal and a prism when the frequency of the far-infrared light changes (see FIG. 8).
Abstract:
An optical sensor includes light sources configured to emit light, a substrate on which the light sources are mounted, the substrate comprising holes in regions on which the light sources are mounted, and a first photodetector configured to receive a first light emitted from a front surface of each of the light sources, the first light being reflected or scattered from an object. The optical sensor further includes at least one second photodetector configured to receive a second light emitted from a rear surface of each of the light sources, the second light passing through the holes corresponding to the light sources.
Abstract:
A system and method that images biological samples and uses chromophores to analyze the imaged samples. The chromophore analysis can be done by itself or in conjunction with fluorophore analysis in High Content Imaging systems. To perform chromophore analysis the biological samples can be labeled with different chromophores and imaged using transmitted light that is at least partially absorbed by the chromophores. To also perform fluorophore analysis the samples can also be labeled with fluorophores that are excited by excitation light. The chromophore analysis and fluorophore analysis can be performed separately or concurrently using a High Content Imaging system. The system provides the expanded capability by illuminating the chromophore-labeled samples with transmitted light of different wavelengths and automatically detecting the images which represent the differential absorption of the colored lights by the sample.