公开(公告)号：US20220019028A1

公开(公告)日：2022-01-20

申请号：US17375688

申请日：2021-07-14

Applicant: Eric Todd Marple ,  Kirk David Urmey ,  John Meckert

Inventor： Eric Todd Marple ,  Kirk David Urmey ,  John Meckert

IPC: G02B6/293 ,  G02B6/36 ,  G01J3/44

Abstract: A fiber optic probe is provided with a distal sampling end, a proximal end, and light delivery and collection paths therethrough. The probe includes a window disposed at the distal sampling end of the fiber optic probe, the window having a distal end and a proximal end. A lens is disposed at the proximal end of the window, the lens having a distal end, a proximal end, and an aperture. A light delivery optical fiber is provided having a distal end and a proximal end, the distal end being received by the lens aperture. An optical isolator provided within the lens aperture to optically isolate the light delivery path and the light collection path. A collection optical fiber is provided in optical communication with the fiber collection filter. The probe may include a lens collection filter disposed between the window and the lens.

公开(公告)号：US11226474B2

公开(公告)日：2022-01-18

申请号：US16856941

申请日：2020-04-23

Applicant: Trustees of Boston University

Inventor： Jerome Charles Mertz ,  Devin Robert Beaulieu ,  Thomas Gary Bifano

IPC: G01J3/44 ,  G02B21/00 ,  G01N21/64

Abstract: A method for obtaining one or more images of a sample using a microscope includes dividing, using a reverberation cavity, a first one of a plurality of laser pulses into a plurality of sequential sub-pulses, each of the plurality of sequential sub-pulses having a power that is less than a previous one of the plurality of sequential sub-pulses, directing, using the one or more lenses of the microscope, the plurality of sequential sub-pulses onto a portion of the sample to generate a plurality of signals, each of the plurality of signals being associated with a different depth within the sample, and detecting the plurality of signals from the sample to generate one or more images of at least a portion of the sample.

公开(公告)号：US20210404939A1

公开(公告)日：2021-12-30

申请号：US17470504

申请日：2021-09-09

Applicant: Becton, Dickinson and Company

Inventor： Maya Hage ,  Alexander Fainshtein

IPC: G01N15/14 ,  G01J3/44

Abstract: Disclosed herein include systems, devices, methods, and spillover editor for displaying and editing spillover values. A view of a spillover editor can comprise a triangular grid of rows and columns, representing flourophores, each comprising at least one display area and two spillover values. After receiving an adjusted spillover value, an adjusted view of the spillover editor can comprise adjusted plots determined using the adjusted spillover value.

公开(公告)号：US11209346B2

公开(公告)日：2021-12-28

申请号：US16603817

申请日：2018-04-11

Applicant: Unchained Labs

Inventor： Markus Lankers ,  Oliver Valet ,  Lisa Krapf ,  Karsten Goede ,  Steve Bober

IPC: G01J3/44 ,  G01N15/14 ,  G01N21/05 ,  G01N21/85 ,  G02B21/10 ,  G02B21/34 ,  G01N21/65 ,  G01N15/00

Abstract: The invention relates to a liquid cell (1) for the microscopic image capture and Raman spectroscopic material analysis of a particle suspension in a reflected light microscope, having at least the following components: a measuring chamber (2) which has a base (3), a measuring window (5) opposite the base (3), and a seal (6), wherein the base (3) has a planar design at least in one region of the support of the seal (6), and the base (3) has a reflective surface (4) which is provided such that Raman excitation light incident through the measuring window (5) is reflected on the reflective surface (4) in a directed manner such that the background signal in a Raman measurement is reduced and the Raman signal of a particle in a suspension is increased. The invention further relates to a microscope which has such a liquid cell.

公开(公告)号：US11206976B2

公开(公告)日：2021-12-28

申请号：US14884445

申请日：2015-10-15

Applicant: New York University

Inventor： R. Theodore Smith

IPC: G01N33/48 ,  A61B3/00 ,  A61B3/14 ,  A61B3/12 ,  A61B3/10 ,  G01J3/44 ,  G01N21/64 ,  G16H50/20 ,  G16B40/10 ,  G16H30/00

Abstract: A system and method are for analyzing fluorescence of fluorophors in an eye using a non-negative matrix factorization (NMF) method. The NMF method may be initialized with Gaussian mixture model fits and may optionally be constrained to provide identical abundance images for data obtained in response to two or more excitation wavelengths.

公开(公告)号：US11198610B2

公开(公告)日：2021-12-14

申请号：US16116091

申请日：2018-08-29

Applicant: Tsinghua University ,  HON HAI PRECISION INDUSTRY CO., LTD.

Inventor： Ying-Cheng Wang ,  Yuan-Hao Jin ,  Qun-Qing Li ,  Shou-Shan Fan

IPC: C01B32/16 ,  G01J3/44 ,  G01N21/03 ,  G01N21/65 ,  C01B32/168 ,  G03F7/00 ,  B05D5/00

Abstract: The disclosure relates to a method for making carrier for use in single molecule detection. The method includes: providing a rigid substrate; coating a polymer layer on a surface of the rigid substrate, the polymer layer is in semisolid state; transferring a nano-scaled pattern of a template on a surface of the polymer layer by pressing the template on the surface of the polymer layer; obtaining a flexible substrate by removing the template; and applying a metal layer on the flexible substrate. The carrier for use in single molecule detection has a relative higher SERS and can enhance the Raman scattering.

公开(公告)号：US11195276B2

公开(公告)日：2021-12-07

申请号：US16996750

申请日：2020-08-18

Applicant: Cedars-Sinai Medical Center

Inventor： Pramod Butte ,  Adam Mamelak

IPC: G06T7/00 ,  H04N5/33 ,  G01J1/04 ,  G01J1/44 ,  H04N5/235 ,  H04N5/225 ,  A61B1/04 ,  A61B1/00 ,  A61B1/06 ,  A61B1/07 ,  G01J3/44 ,  A61B1/05 ,  G01N21/64 ,  H04N9/04

Abstract: The invention provides systems and methods for imaging a sample. In various embodiments, the invention provides a system comprising an image sensor, a laser for emitting excitation light for an infrared or near-infrared fluorophore, a visible light source, a notch beam splitter, a notch filter, a synchronization module, an image processing unit, an image displaying unit, and light-conducting channels. In various embodiments, the present invention provides a system comprising an image sensor, a laser for emitting excitation light for an infrared or near-infrared fluorophore, a laser clean-up filter, a notch filter, a white light source, an image processing unit, an image displaying unit, and light-conducting channels. In accordance with the present invention, the image sensor can detect both visible light and infrared light.

公开(公告)号：US11193879B2

公开(公告)日：2021-12-07

申请号：US17221545

申请日：2021-04-02

Applicant: 1087 Systems, Inc.

Inventor： Matthias Wagner ,  John Heanue

IPC: G01J3/44 ,  G01N15/14 ,  G01N29/24 ,  G01N21/17 ,  B07C5/34 ,  G01N21/65 ,  G01N21/3563 ,  B01L3/00 ,  G01N21/35 ,  C12N5/071 ,  G01N15/10 ,  G01N15/00

Abstract: An apparatus for sorting cells includes a measurement volume that contains a cell to be measured, a light source that provides light to cause an emission by a fluorescent label attached to the cell, and an optic device that directs the light through the measurement volume. The apparatus flows the cells through the measurement volume such that as the cell flows through the measurement volume, it interacts with the light, resulting in a change in light originating from the measurement volume, the change in light is a fluorescence emission. Another optic device directs a portion of the light originating from the measurement volume to a detector, which detects the portion of the light. A processor operably coupled to the detector generates an estimate of DNA quantity in the cell based on the change in light originating from the measurement volume, and determines a characteristic of the cell from the estimate.

公开(公告)号：US11193828B2

公开(公告)日：2021-12-07

申请号：US17014258

申请日：2020-09-08

Applicant: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE

Inventor： Won Kyoung Lee ,  Hong-Seok Seo

IPC: G01J3/44 ,  G01J3/10 ,  G01N21/65 ,  G01J3/02

Abstract: A spectroscopic apparatus includes a splitter that receives a first detected signal output from a sample to which an incident beam is irradiated, and outputs a reflected signal and a second detected signal by splitting the first detected signal, and a signal processor that receives the reflected signal and the second detected signal, and extracts a Raman signal from the second detected signal in response to the received reflected signal.

公开(公告)号：US20210372933A1

公开(公告)日：2021-12-02

申请号：US16961675

申请日：2019-01-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ya-Hong Xie ,  Owen Liang

IPC: G01N21/65 ,  G01J3/02 ,  G01N33/569 ,  G01N33/574 ,  G01N33/68 ,  G01J3/44

Abstract: Systems and methods for characterizing biological specimens, which may involve identifying a cell type or state corresponding to a disease or health condition of a subject. A biological specimen is subjected to electromagnetic radiation for spectroscopic analysis such as Surface Enhanced Raman Spectroscopy (SERS) to determine the relative abundance of proteins or amino acids in the cells, which is used in a comparison to previously stored relative abundance data of a database to automatically identifies at least one of cell type and/or cell state of the cells (or the disease/health state of the subject with the disease state including the possibility of virus infection, or drug susceptibility of a subject to bacteria or fungus). The method may also be employed with biological entities or cellular structures such as exosomes and even protein or nucleic acid fragments to determine disease states or health states of the subject.