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公开(公告)号：US20220082489A1
公开(公告)日：2022-03-17
申请号：US17304844
申请日：2021-06-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huiming Gu , Shao Qing , Ming Yan , David Vrane
IPC: G01N15/14
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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公开(公告)号：US20220082488A1
公开(公告)日：2022-03-17
申请号：US17304843
申请日：2021-06-26
Applicant: Cytek Biosciences, Inc.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huiming Gu , Shao Qing , Ming Yan , David Vrane
IPC: G01N15/14
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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公开(公告)号：US20200333237A1
公开(公告)日：2020-10-22
申请号：US16920618
申请日：2020-07-03
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane , Ming Yan , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
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公开(公告)号：US10788411B2
公开(公告)日：2020-09-29
申请号：US15494260
申请日：2017-04-21
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Eric Chase , Yung-Chieh Hsieh
Abstract: A system, method, and apparatus are provided for cytometry with dual laser beams. In one example, the method includes directing an incident light beam from a source to enter an optical waveplate; polarizing the incident light beam into a polarized light beam in response to the incident light beam entering through the optical waveplate; directing the polarized light beam to enter a birefringent crystal; separating the polarized light beam into an ordinary light beam and an extraordinary light beam in response to the polarized light beam entering the birefringent crystal; directing the ordinary light beam and the extraordinary light beam to enter a lens; focusing the ordinary light beam and the extraordinary light beam into dual light beams separated by a beam displacement; and coupling the dual light beams to form a sample region having substantially uniform light intensity to analyze moving particles in the particle analyzer.
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公开(公告)号：US10739245B2
公开(公告)日：2020-08-11
申请号：US15498397
申请日：2017-04-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： David Vrane , Ming Yan , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.
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Patent Agency Ranking
公开(公告)号：US12209948B2
公开(公告)日：2025-01-28
申请号：US17304843
申请日：2021-06-26
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Maria Jaimes , Zhenyu Zhang , Jian Zhou , Huimin Gu , Qing Shao , Ming Yan , David Vrane
IPC: G01N15/14 , G01N15/00 , G01N15/1434 , G01N15/01
Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.
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公开(公告)号：US20250020639A1
公开(公告)日：2025-01-16
申请号：US18747407
申请日：2024-06-18
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Yu Rong , Jing Dai , Xingyong Wu , Rong Zhang , Peter Robles , Su Chen , Bill Godfrey , Ming Yan
IPC: G01N33/533 , C09K11/06 , G01N15/01 , G01N15/10 , G01N15/14 , G01N33/543 , G01N33/569
Abstract: In one embodiment, a method to synthesize highly fluorescent complexes is disclosed. The highly fluorescent complexes are synthesized by using inorganic nanoparticles, coupling agents, linkers, and fluorescent dye molecules. The unique nanoparticle-dye complexes (referred to as “SN-dye”) can provide ultra-bright fluorescent labelling. This is demonstrated by coupling the complexes to the antibody and subsequently using the conjugated antibody for more sensitive immunological detection in flow cytometry applications.
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公开(公告)号：US11913868B2
公开(公告)日：2024-02-27
申请号：US17302651
申请日：2021-05-10
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC: G01N15/00 , G01J3/00 , G01J1/08 , G01N21/47 , G01N15/14 , G01J3/02 , G01J3/36 , G01N15/10 , G01J3/12
CPC classification number: G01N15/1436 , G01J1/08 , G01J3/021 , G01J3/0208 , G01J3/0218 , G01J3/0256 , G01J3/0291 , G01J3/36 , G01N15/1434 , G01N15/1459 , G01N21/47 , G01J2003/1213 , G01N2015/1006 , G01N2015/145
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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公开(公告)号：US11169076B2
公开(公告)日：2021-11-09
申请号：US15659610
申请日：2017-07-25
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase
IPC: G01N15/00 , G01N21/00 , G01J3/00 , G01N15/14 , G01J1/08 , G01J3/02 , G01J3/36 , G01N21/47 , G01J3/12 , G01N15/10
Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.
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公开(公告)号：US20180299367A1
公开(公告)日：2018-10-18
申请号：US15942430
申请日：2018-03-30
Applicant: CYTEK BIOSCIENCES, INC.
Inventor： Ming Yan , Yung-Chieh Hsieh , David Vrane , Eric Chase , Wenbin Jiang
Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.
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