公开(公告)号：US08675192B2

公开(公告)日：2014-03-18

申请号：US12933350

申请日：2009-03-20

Applicant: Nicolas Ugolin ,  Denis Menut ,  Julien Le Meur ,  Nadine Coulon ,  Sylvie Chevillard ,  Emilie Bosc ,  Jean-Marc Joseph Desaulniers

Inventor： Nicolas Ugolin ,  Denis Menut ,  Julien Le Meur ,  Nadine Coulon ,  Sylvie Chevillard ,  Emilie Bosc ,  Jean-Marc Joseph Desaulniers

IPC: G01J3/30

CPC classification number: G01N21/718 ,  G01N21/6452

Abstract: The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following, steps: a) at least two laser beams (F″) are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and analyzed for each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the qualified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations of the latter in mixtures of the quantified element and exogenous element in known proportions.

Abstract translation: 本发明涉及用于生物分析的平面介质的表面或体内的生物分子靶的高速定量测定的装置和方法。 根据本发明的方法包括以下步骤：a）至少两个激光束（F“）通过这些光束的同时交叉来聚焦并重叠在所述介质的每个测量点上以提取所含的热等离子体 （P），包括目标中存在的测量化学元素和另外一种对目标外源的化学元素，并以该已知量存在于该介质上; b）在测量这些光线的亮度的同时，对每个测量点检测并分析对应于量化元素和外源元素的每个等离子体的发光射线; 然后c）通过先前校准合格元素的射线来确定量化元件的每个测量点中的浓度，以确定特定于所述元素的射线的亮度与后者的亮度之间的相关性， 已知比例的量化元素和外源元素。

公开(公告)号：US08486866B2

公开(公告)日：2013-07-16

申请号：US12441225

申请日：2007-09-14

Applicant: Nicolas Ugolin ,  Denis Menut ,  Julien Le Meur ,  Pascal Wodling ,  Sylvie Chevillard

Inventor： Nicolas Ugolin ,  Denis Menut ,  Julien Le Meur ,  Pascal Wodling ,  Sylvie Chevillard

IPC: C40B50/14 ,  G01J3/30 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

CPC classification number: G01N21/718 ,  B33Y80/00

Abstract: The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip (1) of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points (2) each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam (18) is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be quantified and given concentrations of this element.

Abstract translation: 本发明特别涉及一种用于定量测量生物分子靶标的方法，该方法已经沉积在具有由靶标杂交的探针基质的类型的生物芯片（1）上，该基质包含多个测量点（2） 每个包括多个探针，其特征在于，其包括以下步骤：a）至少一个激光束（18）聚焦到每个测量点上，以便从其中提取包含要量化的化学元素的热限制等离子体， 存在于目标物中，任选地存在于探针中; b）通过测量这些线的相应强度，对每个测量点检测和分析来自等离子体的发光线; 然后c）通过先前校准线确定要量化的元素和给定浓度的特异性线之间的相关性来确定元件或其中包含在靶中的组的每个测量点中的浓度 的元素。

公开(公告)号：US20130101665A1

公开(公告)日：2013-04-25

申请号：US13640202

申请日：2011-04-08

Applicant: Nicolas Ugolin ,  Caroline Falck ,  Emilie Lefevre ,  Sylvie Chevillard ,  Carine Tisne-Vicrobeck

Inventor： Nicolas Ugolin ,  Caroline Falck ,  Emilie Lefevre ,  Sylvie Chevillard ,  Carine Tisne-Vicrobeck

IPC: C07K16/00

Abstract: Novel chimeric molecules, termed “half-antibodies”, which are capable of self-assembling to form an epitope recognition site. Using these half-antibodies or a vesicle, a viral particle, a composition or a kit thereof, for therapeutic applications, such as the prevention or treatment of cancers, genetic diseases, infectious diseases, and for in vitro diagnostic applications and detecting biological molecules. The half-antibodies include at least two chimeric molecules A and B, each has a polypeptide domain characteristic of a variable domain of a heavy chain or of a light chain of an antibody, and a nucleotide domain, the nucleotide domain of A and that of B being capable of pairing into a double stranded structure. Biologically active nucleic sequences can be grafted onto these chimeric molecules to prevent the expression of target genes in the interior of a human or non-human mammalian cell.

Abstract translation: 称为“半抗体”的新型嵌合分子，其能够自组装形成表位识别位点。 使用这些半抗体或囊泡，病毒颗粒，组合物或试剂盒，用于治疗应用，例如预防或治疗癌症，遗传疾病，感染性疾病和用于体外诊断应用和检测生物分子。 半抗体包括至少两个嵌合分子A和B，它们各自具有特征在于抗体的重链或轻链的可变结构域的多肽结构域，以及核苷酸结构域，A的核苷酸结构域和 B能够配对成双链结构。 生物活性核酸序列可以接枝到这些嵌合分子上以防止靶基因在人或非人哺乳动物细胞内部的表达。

公开(公告)号：US08388706B2

公开(公告)日：2013-03-05

申请号：US12742980

申请日：2008-11-14

Applicant: Nicolas Ugolin

Inventor： Nicolas Ugolin

IPC: C10J3/08 ,  C10J3/18 ,  B01J7/00 ,  C01B6/24 ,  C01B3/02

CPC classification number: B01J19/129 ,  B01J8/0055 ,  B01J8/44 ,  B01J19/088 ,  B01J19/10 ,  B01J19/126 ,  B01J19/127 ,  B01J19/18 ,  B01J19/1806 ,  B01J19/20 ,  B01J19/2405 ,  B01J19/26 ,  B01J2208/00884 ,  B01J2219/0809 ,  B01J2219/0815 ,  B01J2219/082 ,  B01J2219/0826 ,  B01J2219/0828 ,  B01J2219/083 ,  B01J2219/0833 ,  B01J2219/0841 ,  B01J2219/0852 ,  B01J2219/0854 ,  B01J2219/0856 ,  B01J2219/0858 ,  B01J2219/086 ,  B01J2219/0866 ,  B01J2219/0867 ,  B01J2219/0869 ,  B01J2219/0871 ,  B01J2219/0879 ,  B01J2219/0892 ,  B01J2219/0896 ,  B01J2219/0898 ,  B01J2219/1215 ,  B01J2219/1227 ,  B01J2219/1239 ,  B01J2219/1242 ,  B01J2219/1245 ,  B01J2219/1266 ,  B01J2219/1269 ,  B01J2219/1272 ,  B01J2219/1284 ,  B01J2219/1296 ,  B01J2219/185 ,  B01J2219/1943 ,  C10B19/00 ,  C10J3/487 ,  C10J3/66 ,  C10J2300/0916 ,  C10J2300/094 ,  C10J2300/1284 ,  C10J2300/16 ,  C10L9/083 ,  Y02E50/15 ,  Y02E60/364 ,  Y02P20/134 ,  Y02P20/145 ,  Y02T50/678

Abstract: A system uses thermal solar energy coupled with microwaves and plasma for producing carbon monoxide (CO) and dihydrogen (H2) from carbonated compounds (biomass, domestic waste, sludge from waste water, fossil coal), wherein the obtained gaseous mixture yields, amongst others, hydrocarbon fuels (olefins, paraffin), esters, and alcohols via a Fischer-Tropsch synthesis. In a first step the carbonated compounds are roasted and pyrolized to produce char and dry coal, and a mixture of superheated gases containing CO2, steam, tars and non-condensable volatile materials. The method includes in a second step, and from the pyrolyis products (char or coal, gas mixture), generating a syngas substantially containing a mixture of carbon monoxide and dihydrogen, the mixture being used in Fischer-Tropsch synthesis units. After the Fischer-Tropsch step, the synthesis products are separated in a distillation column after heating in solar furnaces of mixed furnaces (solar/microwave).

Abstract translation: 一个系统使用与微波和等离子体耦合的热太阳能从碳酸化合物（生物质能，生活废物，废水，化石煤泥）中产生一氧化碳（CO）和二氢（H2），其中获得的气体混合物产生 ，烃燃料（烯烃，石蜡），酯和醇通过费 - 托合成。 在第一步中，将碳酸化合物焙烧和热解以产生炭和干煤，以及含有CO 2，蒸汽，焦油和不可冷凝挥发性物质的过热气体的混合物。 该方法包括在第二步骤中，从热解产物（炭或煤，气体混合物）中产生基本上含有一氧化碳和二氢的混合物的合成气，该混合物用于费 - 托合成装置。 在费 - 托步骤之后，在混合炉（太阳能/微波炉）的太阳能炉中加热后，在蒸馏塔中分离合成产物。

公开(公告)号：US08211693B2

公开(公告)日：2012-07-03

申请号：US12700680

申请日：2010-02-04

Applicant: Nicolas Ugolin ,  Sylvie Chevillard ,  Catherine Ory ,  Jerome Lebeau

Inventor： Nicolas Ugolin ,  Sylvie Chevillard ,  Catherine Ory ,  Jerome Lebeau

IPC: C12M1/34

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  B01L2300/0819 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0478 ,  G01N1/405 ,  G01N27/44773 ,  G01N27/44782

Abstract: The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.

Abstract translation: 本发明涉及用于分离和/分析溶解在复合混合物中的几种分子靶的装置，其特征在于其包括a）微柱基质，其中每个微柱（2）包含固定的分子探针，用于保留 通过特异性探针/靶连接物包含在复合物混合物中的特定分子靶，b）用于将引入本发明装置的复合物混合物循环到a），c）中定义的基质的每个微柱的第一毛细管网（3） 第二毛细管网络（4），用于在洗脱后使保留在微柱上的分子靶朝向传感器（5）循环，用于进行其回收和/或分析，以及d）如果需要，传感器（5） ，优选以质谱仪的形式，用于进行不同分子靶标的回收和/或分析。

公开(公告)号：US20110109904A1

公开(公告)日：2011-05-12

申请号：US12933350

申请日：2009-03-20

Applicant: Nicolas Ugolin ,  Denis Menut ,  Julien Le Meur ,  Nadine Coulon ,  Sylvie Chevillard ,  Emilie Bosc ,  Jean-Marc-Joseph Desaulniers

Inventor： Nicolas Ugolin ,  Denis Menut ,  Julien Le Meur ,  Nadine Coulon ,  Sylvie Chevillard ,  Emilie Bosc ,  Jean-Marc-Joseph Desaulniers

IPC: G01J3/443

CPC classification number: G01N21/718 ,  G01N21/6452

Abstract: The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following, steps: a) at least two laser beams (F″) are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and anglyset each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the qualified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations the latter in mixtures of the quantified element and the exogenous element in known proportions.

Abstract translation: 本发明涉及用于生物分析的平面介质的表面或体内的生物分子靶的高速定量测定的装置和方法。 根据本发明的方法包括以下步骤：a）至少两个激光束（F“）通过这些光束的同时交叉来聚焦并覆盖在所述介质的每个测量点上以提取所含有的热等离子体 P），包括目标中存在的测量化学元素和另外一种对目标外源的化学元素，并以该已知量存在于该介质上; b）在测量这些光线的亮度的同时检测对应于量化元件和外源元件的每个等离子体的发光射线，并对每个测量点进行角度测量; 然后c）量化元素的每个测量点中的浓度通过先前校准合格元素的射线来确定，以确定特定于所述元素的射线的亮度与后者在混合物中的浓度之间的相关性 量化元素和已知比例的外源元素。

公开(公告)号：US20100258429A1

公开(公告)日：2010-10-14

申请号：US12742980

申请日：2008-11-14

Applicant: Nicolas Ugolin

Inventor： Nicolas Ugolin

IPC: A62D3/178 ,  B01J19/08

CPC classification number: B01J19/129 ,  B01J8/0055 ,  B01J8/44 ,  B01J19/088 ,  B01J19/10 ,  B01J19/126 ,  B01J19/127 ,  B01J19/18 ,  B01J19/1806 ,  B01J19/20 ,  B01J19/2405 ,  B01J19/26 ,  B01J2208/00884 ,  B01J2219/0809 ,  B01J2219/0815 ,  B01J2219/082 ,  B01J2219/0826 ,  B01J2219/0828 ,  B01J2219/083 ,  B01J2219/0833 ,  B01J2219/0841 ,  B01J2219/0852 ,  B01J2219/0854 ,  B01J2219/0856 ,  B01J2219/0858 ,  B01J2219/086 ,  B01J2219/0866 ,  B01J2219/0867 ,  B01J2219/0869 ,  B01J2219/0871 ,  B01J2219/0879 ,  B01J2219/0892 ,  B01J2219/0896 ,  B01J2219/0898 ,  B01J2219/1215 ,  B01J2219/1227 ,  B01J2219/1239 ,  B01J2219/1242 ,  B01J2219/1245 ,  B01J2219/1266 ,  B01J2219/1269 ,  B01J2219/1272 ,  B01J2219/1284 ,  B01J2219/1296 ,  B01J2219/185 ,  B01J2219/1943 ,  C10B19/00 ,  C10J3/487 ,  C10J3/66 ,  C10J2300/0916 ,  C10J2300/094 ,  C10J2300/1284 ,  C10J2300/16 ,  C10L9/083 ,  Y02E50/15 ,  Y02E60/364 ,  Y02P20/134 ,  Y02P20/145 ,  Y02T50/678

Abstract: A system uses thermal solar energy coupled with microwaves and plasma for producing carbon monoxide (CO) and dihydrogen (H2) from carbonated compounds (biomass, domestic waste, sludge from waste water, fossil coal), wherein the obtained gaseous mixture yields, amongst others, hydrocarbon fuels (olefins, paraffin), esters, and alcohols via a Fischer-Tropsch synthesis. In a first step the carbonated compounds are roasted and pyrolized to produce char and dry coal, and a mixture of superheated gases containing CO2, steam, tars and non-condensable volatile materials. The method includes in a second step, and from the pyrolyis products (char or coal, gas mixture), generating a syngas substantially containing a mixture of carbon monoxide and dihydrogen, the mixture being used in Fischer-Tropsch synthesis units. After the Fischer-Tropsch step, the synthesis products are separated in a distillation column after heating in solar furnaces of mixed furnaces (solar/microwave).

Abstract translation: 一个系统使用与微波和等离子体耦合的热太阳能从碳酸化合物（生物质能，生活废物，废水，化石煤泥）中产生一氧化碳（CO）和二氢（H2），其中获得的气体混合物产生 ，烃燃料（烯烃，石蜡），酯和醇通过费 - 托合成。 在第一步中，将碳酸化合物焙烧和热解以产生炭和干煤，以及含有CO 2，蒸汽，焦油和不可冷凝挥发性物质的过热气体的混合物。 该方法包括在第二步骤中，从热解产物（炭或煤，气体混合物）中产生基本上含有一氧化碳和二氢的混合物的合成气，该混合物用于费 - 托合成装置。 在费 - 托步骤之后，在混合炉（太阳能/微波炉）的太阳能炉中加热后，在蒸馏塔中分离合成产物。

公开(公告)号：US20080182758A1

公开(公告)日：2008-07-31

申请号：US11892672

申请日：2007-08-24

Applicant: Nicolas Ugolin ,  Sylvie Chevillard ,  Alexandre Coutant ,  Jerome Lebeau

Inventor： Nicolas Ugolin ,  Sylvie Chevillard ,  Alexandre Coutant ,  Jerome Lebeau

IPC: C40B30/04 ,  C40B60/12 ,  C40B40/08 ,  C40B50/00

CPC classification number: G01N33/5438 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0822 ,  B01L2300/0864 ,  B01L2300/0867 ,  C12Q1/6837 ,  C12Q2565/513 ,  C12Q2545/114

Abstract: The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.

Abstract translation: 本发明涉及一种分析复合混合物中所含的分子靶的方法，包括以下步骤：a）使待分析的分子靶标的混合物与不同类型的主要探针的阵列接触，由此每种 形成阵列的主要探针可以在能够在分子靶标和主要探针之间进行特异性结合的条件下特异性地结合选自分子靶标的靶标类型; b）任选地消除不与分子靶特异性结合的主要探针; c）分离特异性结合在探针/靶复合体中的分子靶和主要探针，例如恢复代表要分析的分子靶标的指纹的主要探针阵列; 和d）定量分析步骤c中洗脱的主要探针。