公开(公告)号：US20160211126A1

公开(公告)日：2016-07-21

申请号：US14918269

申请日：2015-10-20

Applicant: Sarah Trimpin

Inventor： Sarah Trimpin

IPC: H01J49/04 ,  H01J49/16

CPC classification number: H01J49/044 ,  H01J27/24 ,  H01J49/0468 ,  H01J49/164

Abstract: Disclosed herein are systems and methods for mass spectrometry using laserspray ionization (LSI). LSI can create multiply-charged ions at atmospheric pressure for analysis and allows for analysis of high molecular weight molecules including molecules over 4000 Daltons. The analysis can be solvent-based or solvent-free. Solvent-free analysis following LSI allows for improved spatial resolution beneficial in surface and/or tissue imaging.

Abstract translation: 本文公开了使用激光喷射电离（LSI）的质谱的系统和方法。 LSI可以在大气压下产生多电荷离子进行分析，并允许分析高分子量分子，包括超过4000道尔顿的分子。 分析可以是溶剂型或无溶剂型。 在LSI之后的无溶剂分析允许有利于表面和/或组织成像的改善的空间分辨率。

公开(公告)号：US09105458B2

公开(公告)日：2015-08-11

申请号：US13899552

申请日：2013-05-21

Applicant: Sarah Trimpin ,  Ellen dela Victoria Inutan

Inventor： Sarah Trimpin ,  Ellen dela Victoria Inutan

IPC: H01J49/16 ,  H01J49/10 ,  G01N27/62

CPC classification number: G01N27/622 ,  H01J49/04 ,  H01J49/10 ,  H01J49/16

Abstract: An ionization method for use with mass spectrometry or ion mobility spectrometry is a small molecule compound(s) as a matrix into which is incorporated analyte. The matrix has attributes of sublimation or evaporation when placed in vacuum at or near room temperature and produces both positive and negative charges. Placing the sample into a region of sub-atmospheric pressure, the region being in fluid communication with the vacuum of the mass spectrometer or ion mobility spectrometer, produces gas-phase ions of the analyte for mass-to-charge or drift-time analysis without use of a laser, high voltage, particle bombardment, or a heated ion transfer region. This matrix and vacuum assisted ionization process can operate from atmosphere or vacuum and produces ions from large (e.g. proteins) and small molecules (e.g. drugs) with charge states similar to those observed in electrospray ionization.

Abstract translation: 用于质谱或离子迁移谱的离子化方法是作为基质的小分子化合物，其中加入有分析物。 当置于室温或室温附近时，该基质具有升华或蒸发的特性，并产生正电荷和负电荷。 将样品置于亚大气压力区域内，该区域与质谱仪或离子迁移谱仪的真空流体连通，产生分析物的气相离子，用于质量 - 电荷或漂移 - 时间分析，无需 使用激光，高电压，粒子轰击或加热的离子转移区域。 该基质和真空辅助电离过程可以从大气或真空中进行操作，并从大的（例如蛋白质）和小分子（例如药物）产生离子，其电荷状态类似于在电喷雾电离中观察到的电荷状态。

公开(公告)号：US20140166875A1

公开(公告)日：2014-06-19

申请号：US14073731

申请日：2013-11-06

Applicant: Sarah Trimpin

Inventor： Sarah Trimpin

IPC: H01J49/10

CPC classification number: H01J49/0468 ,  H01J49/04 ,  H01J49/10 ,  H01J49/24

Abstract: A multiplex system and method for achieving high throughput analysis of samples using solvent assisted ionization inlet includes an ionizing system with a heated inlet channel and a pressure differential across the inlet channel, pipet tips serially aligned with the inlet to a mass spectrometer, and a system of mapping data generated by mass spectrometry.

Abstract translation: 使用溶剂辅助电离入口实现样品的高通量分析的多路复用系统和方法包括具有加热入口通道和跨入口通道的压力差的离子化系统，与质谱仪的入口串联对准的移液管尖端，以及系统 通过质谱法生成的测绘数据。

公开(公告)号：US20130306856A1

公开(公告)日：2013-11-21

申请号：US13899552

申请日：2013-05-21

Applicant: Sarah Trimpin ,  Ellen dela Victoria Inutan

Inventor： Sarah Trimpin ,  Ellen dela Victoria Inutan

IPC: H01J49/16

CPC classification number: G01N27/622 ,  H01J49/04 ,  H01J49/10 ,  H01J49/16

Abstract: An ionization method for use with mass spectrometry or ion mobility spectrometry is a small molecule compound(s) as a matrix into which is incorporated analyte. The matrix has attributes of sublimation or evaporation when placed in vacuum at or near room temperature and produces both positive and negative charges. Placing the sample into a region of sub-atmospheric pressure, the region being in fluid communication with the vacuum of the mass spectrometer or ion mobility spectrometer, produces gas-phase ions of the analyte for mass-to-charge or drift-time analysis without use of a laser, high voltage, particle bombardment, or a heated ion transfer region. This matrix and vacuum assisted ionization process can operate from atmosphere or vacuum and produces ions from large (e.g. proteins) and small molecules (e.g. drugs) with charge states similar to those observed in electrospray ionization.

Abstract translation: 用于质谱或离子迁移谱的离子化方法是作为基质的小分子化合物，其中加入有分析物。 当置于室温或室温附近时，该基质具有升华或蒸发的特性，并产生正电荷和负电荷。 将样品置于亚大气压力区域内，该区域与质谱仪或离子迁移谱仪的真空流体连通，产生分析物的气相离子，用于质量 - 电荷或漂移 - 时间分析，无需 使用激光，高电压，粒子轰击或加热的离子转移区域。 该基质和真空辅助电离过程可以从大气或真空中进行操作，并从大的（例如蛋白质）和小分子（例如药物）产生离子，其电荷状态类似于在电喷雾电离中观察到的电荷状态。

公开(公告)号：US11430648B2

公开(公告)日：2022-08-30

申请号：US16861214

申请日：2020-04-29

Applicant: Sarah Trimpin ,  Ellen dela Victoria Inutan

Inventor： Sarah Trimpin ,  Ellen dela Victoria Inutan

IPC: H01J49/16 ,  H01J49/10 ,  G01N27/622 ,  H01J49/04

Abstract: An ionization method for use with mass spectrometry or ion mobility spectrometry is a small molecule compound(s) as a matrix into which is incorporated analyte. The matrix has attributes of sublimation or evaporation when placed in vacuum at or near room temperature and produces both positive and negative charges. Placing the sample into a region of sub-atmospheric pressure, the region being in fluid communication with the vacuum of the mass spectrometer or ion mobility spectrometer, produces gas-phase ions of the analyte for mass-to-charge or drift-time analysis without use of a laser, high voltage, particle bombardment, or a heated ion transfer region. This matrix and vacuum assisted ionization process can operate from atmosphere or vacuum and produces ions from large (e.g. proteins) and small molecules (e.g. drugs) with charge states similar to those observed in electrospray ionization.

公开(公告)号：US20200343084A1

公开(公告)日：2020-10-29

申请号：US16861214

申请日：2020-04-29

Applicant: Sarah Trimpin ,  Ellen dela Victoria Inutan

Inventor： Sarah Trimpin ,  Ellen dela Victoria Inutan

IPC: H01J49/16 ,  H01J49/10 ,  G01N27/62 ,  H01J49/04

Abstract: An ionization method for use with mass spectrometry or ion mobility spectrometry is a small molecule compound(s) as a matrix into which is incorporated analyte. The matrix has attributes of sublimation or evaporation when placed in vacuum at or near room temperature and produces both positive and negative charges. Placing the sample into a region of sub-atmospheric pressure, the region being in fluid communication with the vacuum of the mass spectrometer or ion mobility spectrometer, produces gas-phase ions of the analyte for mass-to-charge or drift-time analysis without use of a laser, high voltage, particle bombardment, or a heated ion transfer region. This matrix and vacuum assisted ionization process can operate from atmosphere or vacuum and produces ions from large (e.g. proteins) and small molecules (e.g. drugs) with charge states similar to those observed in electrospray ionization.

公开(公告)号：US09824872B2

公开(公告)日：2017-11-21

申请号：US15181300

申请日：2016-06-13

Applicant: Sarah Trimpin

Inventor： Sarah Trimpin

IPC: H01J49/00 ,  H01J49/04 ,  H01J49/24 ,  H01J49/10

CPC classification number: H01J49/0468 ,  H01J49/04 ,  H01J49/10 ,  H01J49/24

Abstract: A multiplex system and method for achieving high throughput analysis of samples using solvent assisted ionization inlet includes an ionizing system with a heated inlet channel and a pressure differential across the inlet channel, pipet tips serially aligned with the inlet to a mass spectrometer, and a system of mapping data generated by mass spectrometry.

公开(公告)号：US20140027631A1

公开(公告)日：2014-01-30

申请号：US13881684

申请日：2011-10-25

Applicant: Sarah Trimpin

Inventor： Sarah Trimpin

IPC: H01J49/16

CPC classification number: H01J49/164 ,  G01N27/622 ,  H01J49/165 ,  H01J49/24 ,  H01J49/40

Abstract: Disclosed herein are systems and methods that allow analysis of macromolecular structures using laserspray ionization at intermediate pressure or high vacuum using commercially available mass spectrometers with or without modification and with the application of heat. The systems and methods produce multiply-charged ions for improved analysis in mass spectrometry.

Abstract translation: 本文公开了允许使用具有或不具有改性和加热的市售质谱仪在中压或高真空下使用激光喷射电离分析大分子结构的系统和方法。 该系统和方法产生多重带电离子，以改进质谱分析。

公开(公告)号：US20130214154A1

公开(公告)日：2013-08-22

申请号：US13819487

申请日：2011-09-01

Applicant: Charles Nehemiah McEwen ,  Vincent Salvatore Pagnotti ,  Sarah Trimpin

Inventor： Charles Nehemiah McEwen ,  Vincent Salvatore Pagnotti ,  Sarah Trimpin

IPC: H01J49/10

CPC classification number: H01J49/10 ,  H01J49/0404 ,  H01J49/0468

Abstract: An ionizing system includes a channel having an inlet disposed in a first pressure region and an outlet disposed in a second pressure region, a pressure of the first pressure region being greater than a pressure of the second pressure region. A heater is coupled to the channel and configured to heat the channel. A device is configured to introduce an analyte into the channel where the analyte is ionized.

Abstract translation: 电离系统包括具有设置在第一压力区域中的入口和设置在第二压力区域中的出口的通道，第一压力区域的压力大于第二压力区域的压力。 加热器耦合到通道并且被配置为加热通道。 一种装置被配置为将分析物引入分析物被离子化的通道中。

公开(公告)号：US20120085903A1

公开(公告)日：2012-04-12

申请号：US13376132

申请日：2010-06-03

Applicant: Sarah Trimpin

Inventor： Sarah Trimpin

IPC: H01J49/26

CPC classification number: H01J49/044 ,  H01J27/24 ,  H01J49/0468 ,  H01J49/164

Abstract: Disclosed herein are systems and methods for mass spectrometry using laserspray ionization (LSI). LSI can create multiply-charged ions at atmospheric pressure for analysis and allows for analysis of high molecular weight molecules including molecules over 4000 Daltons. The analysis can be solvent-based or solvent-free. Solvent-free analysis following LSI allows for improved spatial resolution beneficial in surface and/or tissue imaging.

Abstract translation: 本文公开了使用激光喷射电离（LSI）的质谱的系统和方法。 LSI可以在大气压下产生多电荷离子进行分析，并允许分析高分子量分子，包括超过4000道尔顿的分子。 分析可以是溶剂型或无溶剂型。 在LSI之后的无溶剂分析允许有利于表面和/或组织成像的改善的空间分辨率。