公开(公告)号：US09687152B2

公开(公告)日：2017-06-27

申请号：US14009473

申请日：2012-04-05

Applicant: Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

Inventor： Haishan Zeng ,  Harvey Lui ,  David McLean ,  Anthony Lee ,  Hequn Wang ,  Shuo Tang

IPC: A61B5/00 ,  A61B5/06 ,  G02B21/00 ,  G02B21/06 ,  G01J3/44 ,  G01J3/02 ,  A61B18/18 ,  A61N5/06

CPC classification number: A61B5/0064 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/4836 ,  A61B5/6831 ,  A61B5/6832 ,  A61B18/18 ,  A61N5/06 ,  A61N5/0616 ,  G01J3/0218 ,  G01J3/027 ,  G01J3/44 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/06

Abstract: A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength λ; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals com-prises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.

公开(公告)号：US09654745B2

公开(公告)日：2017-05-16

申请号：US13635682

申请日：2011-03-17

Applicant: Haishan Zeng ,  Yasser Fawzy

Inventor： Haishan Zeng ,  Yasser Fawzy

IPC: A61B1/06 ,  A61B5/00 ,  H04N9/04 ,  A61B1/00 ,  G01J3/10 ,  G01J3/28 ,  G01J3/36 ,  H04N5/33 ,  A61B1/04 ,  H04N5/225

CPC classification number: H04N9/045 ,  A61B1/00009 ,  A61B1/00057 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0607 ,  A61B1/063 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/0676 ,  A61B1/0684 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/0086 ,  G01J3/10 ,  G01J3/28 ,  G01J3/36 ,  G01J2003/2866 ,  G01N21/35 ,  H04N5/332 ,  H04N9/646 ,  H04N2005/2255

Abstract: Methods and apparatus for video rate or near video rate quantitative imaging of tissue physiological and morphological properties from visible/NIR light spectral images obtain rapid multi-spectral reflectance images by illuminating with a series of spectra containing multiple narrow wavelength bands. An iterative light-transport based inversion algorithm may be applied for correcting the intensity of the spectral images from the geometry/coupling effect as well as from the scattering amplitude distortions. The method can produce video rate absorption as well as scattering spectral images that can be further analyzed very rapidly, using matrix-based rapid inversion algorithms to produce more detailed quantitative images containing information relevant to tissue physiology and morphology.

公开(公告)号：US20130012794A1

公开(公告)日：2013-01-10

申请号：US13635682

申请日：2011-03-17

Applicant: Haishan Zeng ,  Yasser Fawzy

Inventor： Haishan Zeng ,  Yasser Fawzy

IPC: A61B1/06 ,  A61B1/04 ,  A61B5/1455

CPC classification number: H04N9/045 ,  A61B1/00009 ,  A61B1/00057 ,  A61B1/00186 ,  A61B1/043 ,  A61B1/05 ,  A61B1/0607 ,  A61B1/063 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/0676 ,  A61B1/0684 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/0086 ,  G01J3/10 ,  G01J3/28 ,  G01J3/36 ,  G01J2003/2866 ,  G01N21/35 ,  H04N5/332 ,  H04N9/646 ,  H04N2005/2255

Abstract: Methods and apparatus for video rate or near video rate quantitative imaging of tissue physiological and morphological properties from visible/NIR light spectral images obtain rapid multi-spectral reflectance images by illuminating with a series of spectra containing multiple narrow wavelength bands. An iterative light-transport based inversion algorithm may be applied for correcting the intensity of the spectral images from the geometry/coupling effect as well as from the scattering amplitude distortions. The method can produce video rate absorption as well as scattering spectral images that can be further analyzed very rapidly, using matrix-based rapid inversion algorithms to produce more detailed quantitative images containing information relevant to tissue physiology and morphology.

Abstract translation: 通过可见/近红外光谱图像对组织生理和形态特征进行视频率或近视频率定量成像的方法和装置通过用包含多个窄波长带的一系列光谱照射获得快速多光谱反射图像。 可以应用基于迭代光传输的反演算法来从几何/耦合效应以及散射振幅失真校正光谱图像的强度。 该方法可以产生视频速率吸收以及可以非常快速地进一步分析的散射光谱图像，使用基于矩阵的快速反演算法来产生包含与组织生理学和形态相关的信息的更详细的定量图像。

公开(公告)号：US20090270702A1

公开(公告)日：2009-10-29

申请号：US11722822

申请日：2006-01-20

Applicant: Haishan Zeng ,  Yasser Sherif Fawzy

Inventor： Haishan Zeng ,  Yasser Sherif Fawzy

IPC: A61B5/1455 ,  A61B6/00

CPC classification number: G01N21/474 ,  A61B1/043 ,  A61B1/2676 ,  A61B5/0066 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0261 ,  A61B5/14542 ,  A61B5/1459 ,  G01J3/02 ,  G01J3/0243 ,  G01J3/0291 ,  G01N21/359 ,  G01N21/4795 ,  G01N2021/4742

Abstract: The present invention provides a new method and device for disease detection, more particularly cancer detection, from the analysis of diffuse reflectance spectra measured in vivo during endoscopic imaging. The measured diffuse reflectance spectra are analyzed using a specially developed light-transport model and numerical method to derive quantitative parameters related to tissue physiology and morphology. The method also corrects the effects of the specular reflection and the varying distance between endoscope tip and tissue surface on the clinical reflectance measurements. The model allows us to obtain the absorption coefficient (μa) and further to derive the tissue micro-vascular blood volume fraction and the tissue blood oxygen saturation parameters. It also allows us to obtain the scattering coefficients (μs and g) and further to derive the tissue micro-particles volume fraction and size distribution parameters.

Abstract translation: 本发明提供了一种用于疾病检测，特别是癌症检测的新方法和装置，其用于在内窥镜成像期间在体内测量的漫反射光谱。 使用特别开发的光传输模型和数值方法分析测量的漫反射光谱，以得出与组织生理学和形态相关的定量参数。 该方法还可以校正镜面反射和内窥镜尖端与组织表面之间距离变化对临床反射率测量的影响。 该模型允许我们获得吸收系数（mua），并进一步导出组织微血管血液体积分数和组织血氧饱和度参数。 它还允许我们获得散射系数（mus和g），并进一步导出组织微粒体积分数和尺寸分布参数。

公开(公告)号：US07190452B2

公开(公告)日：2007-03-13

申请号：US11058045

申请日：2005-02-14

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

IPC: G01J3/28

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract translation: 提供来自物体的同时图像和光谱的光学系统，例如组织样本，诸如计算机芯片的工业对象或可以用诸如显微镜，内窥镜，望远镜或照相机的光学系统观察的任何其它物体 。 在一些实施例中，系统提供对应于对象的原始图像内的各种期望波长范围的多个图像，以及如果需要的方向指针，其可以提供频谱的精确位置的识别 获得，以及增强指向设备的能力。

公开(公告)号：US20050203421A1

公开(公告)日：2005-09-15

申请号：US11053263

申请日：2005-02-07

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Palcic

IPC: G01N21/64 ,  A61B1/00 ,  A61B1/04 ,  G01J3/02 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N23/00 ,  A61B6/00 ,  G21K1/12 ,  H05G1/60

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract translation: 提供来自物体的同时图像和光谱的光学系统，例如组织样本，诸如计算机芯片的工业对象或可以用诸如显微镜，内窥镜，望远镜或照相机的光学系统观察的任何其它物体 。 在一些实施例中，系统提供对应于对象的原始图像内的各种期望波长范围的多个图像，以及如果需要的方向指针，其可以提供频谱的精确位置的识别 获得，以及增强指向设备的能力。

公开(公告)号：US06546272B1

公开(公告)日：2003-04-08

申请号：US09602202

申请日：2000-06-22

Applicant: Nicholas B. MacKinnon ,  Stephen Lam ,  Haishan Zeng ,  Calum E. MacAulay

Inventor： Nicholas B. MacKinnon ,  Stephen Lam ,  Haishan Zeng ,  Calum E. MacAulay

IPC: A61B500

CPC classification number: A61B5/6852 ,  A61B1/00183 ,  A61B5/0066 ,  A61B5/0084 ,  A61B18/12

Abstract: Apparatus for acquiring in vivo images of a site of interest within the internal organs of a body. The apparatus includes an elongate, flexible catheter. The catheter is introducible into the body and has a first end that remains external to the body and a second and positionable adjacent the site of interest. A movable scanning unit having at least one sensor for acquiring images is housed adjacent the second end of the catheter. There is a drive mechanism to control movement of the movable scanning unit from the first external end of the catheter to acquire multiple images of the site of interest. The drive mechanism has a control element extending the length of the catheter lumen adapted for linear movement within the lumen to generate linear or rotational movement of the scanning unit.

Abstract translation: 用于获取身体内部器官内的感兴趣部位的体内图像的装置。 该装置包括细长的柔性导管。 导管可引入体内，并具有保持在身体外部的第一端，并且可以邻近感兴趣部位定位第二端。 具有用于获取图像的至少一个传感器的可移动扫描单元容纳在导管的第二端附近。 存在驱动机构，用于控制可移动扫描单元从导管的第一外端的移动，以获取感兴趣部位的多个图像。 驱动机构具有延伸导管管腔长度的控制元件，其适于在内腔内线性运动，以产生扫描单元的线性或旋转运动。

公开(公告)号：US20130231573A1

公开(公告)日：2013-09-05

申请号：US13521218

申请日：2011-01-21

Applicant: Haishan Zeng ,  Michael Short ,  Stephen Lam ,  Annette McWilliams

Inventor： Haishan Zeng ,  Michael Short ,  Stephen Lam ,  Annette McWilliams

IPC: A61B5/00

CPC classification number: A61B5/0075 ,  A61B5/0071 ,  A61B5/0084 ,  G01N21/65

Abstract: Near-infrared Raman spectroscopy can be applied to identify preneoplastic lesions of the bronchial tree. Real-time in vivo Raman spectra of lung tissues may be obtained with a fiber optic catheter passed down the instrument channel of a bronchoscope. Using prototype apparatus, preneoplastic lesions were detected with sensitivity and specificity of 96 and 91% respectively. The use of Raman spectroscopy apparatus and methods in conjunction with other bronchoscopy imaging modalities can substantially reduce the number of false positive results.

Abstract translation: 近红外拉曼光谱可用于鉴定支气管树瘤的肿瘤前病变。 肺组织的实时体内拉曼光谱可以通过沿支气管镜仪器通道向下传播的光纤导管获得。 使用原型装置，分别检测到96和91％的敏感性和特异性的癌前病变。 使用拉曼光谱设备和方法与其他支气管镜检查成像模式联合可以大大减少假阳性结果的数量。

公开(公告)号：US07253894B2

公开(公告)日：2007-08-07

申请号：US11053263

申请日：2005-02-07

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

IPC: G01J3/28

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract translation: 提供来自物体的同时图像和光谱的光学系统，例如组织样本，诸如计算机芯片的工业对象或可以用诸如显微镜，内窥镜，望远镜或照相机的光学系统观察的任何其它物体 。 在一些实施例中，系统提供对应于对象的原始图像内的各种期望波长范围的多个图像，以及如果需要的方向指针，其可以提供频谱的精确位置的识别 获得，以及增强指向设备的能力。

公开(公告)号：US07115841B2

公开(公告)日：2006-10-03

申请号：US11057965

申请日：2005-02-14

Applicant: Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

Inventor： Haishan Zeng ,  Stephen Lam ,  Branko Mihael Palcic

IPC: H05B3/02

CPC classification number: G01J3/02 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/0235 ,  G01J3/0243 ,  G01J3/0289 ,  G01J3/10 ,  G01J3/36 ,  G01N23/223 ,  G01N2223/076

Abstract: Optical systems that provide for simultaneous images and spectra from an object, such as a tissue sample, an industrial object such as a computer chip, or any other object that can be viewed with an optical system such as a microscope, endoscope, telescope or camera. In some embodiments, the systems provide multiple images corresponding to various desired wavelength ranges within an original image of the object, as well as, if desired, directional pointer(s) that can provide both an identification of the precise location from which a spectrum is being obtained, as well as enhancing the ability to point the device.

Abstract translation: 提供来自物体的同时图像和光谱的光学系统，例如组织样本，诸如计算机芯片的工业对象或可以用诸如显微镜，内窥镜，望远镜或照相机的光学系统观察的任何其它物体 。 在一些实施例中，系统提供对应于对象的原始图像内的各种期望波长范围的多个图像，以及如果需要的方向指针，其可以提供频谱的精确位置的识别 获得，以及增强指向设备的能力。