公开(公告)号：US11260943B2

公开(公告)日：2022-03-01

申请号：US16719371

申请日：2019-12-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Axel Scherer

IPC: B63C11/02 ,  G01N27/22 ,  G01N21/41

Abstract: Methods and devices including implantable micro-sensors used to detect tissue-dissolved inert gas and to detect microbubble formation to avoid Caisson disease are described. The disclosed methods and devices are based on measuring the refractive index changes in hydrophobic liquids after absorbing an inert gas such as nitrogen. The changes in the refractive index are based on implementing one of an interferometry, optical microcavity resonance shift, a photonic crystal resonance, a beam deflection, a resonance tuning or detuning, an amplitude change, or an intensity change method.

公开(公告)号：US20210363581A1

公开(公告)日：2021-11-25

申请号：US17125870

申请日：2020-12-17

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/70

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US10770170B2

公开(公告)日：2020-09-08

申请号：US15914356

申请日：2018-03-07

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N33/48 ,  G01N33/50 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64 ,  C12Q1/6825 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US10457937B2

公开(公告)日：2019-10-29

申请号：US15885352

申请日：2018-01-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA ,  CURATE BIOSCIENCES LLC

Inventor： Emil P. Kartalov ,  Cheng-Chung Lee ,  Paul Predki

IPC: C12P19/34 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6874 ,  B01L3/00 ,  C12Q1/6853

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the devices described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein. The micro-isolation devices can comprise a unique combination of barcodes in each microfluidics well, allowing two-dimensional mapping of genetic information.

公开(公告)号：US10077475B2

公开(公告)日：2018-09-18

申请号：US14633094

申请日：2015-02-26

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6851 ,  C12Q1/70

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US10066263B2

公开(公告)日：2018-09-04

申请号：US15623974

申请日：2017-06-15

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Emil P. Kartalov

IPC: C12Q1/68 ,  C12Q1/686 ,  C07H21/04 ,  G01N21/64

CPC classification number: C12Q1/686 ,  B01J2219/00659 ,  C07H21/04 ,  G01N2021/6417

Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.