公开(公告)号：US11761968B2

公开(公告)日：2023-09-19

申请号：US17208564

申请日：2021-03-22

Applicant: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED

Inventor： Brett Holmquist ,  Nigel Clarke

IPC: G01N33/82 ,  G01N33/68 ,  H01J49/00

CPC classification number: G01N33/82 ,  G01N33/6848 ,  G01N33/6851 ,  H01J49/0027 ,  G01N2560/00 ,  H01J49/00 ,  Y10T436/212

Abstract: The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry.

公开(公告)号：US20190250175A1

公开(公告)日：2019-08-15

申请号：US16390989

申请日：2019-04-22

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Nigel J. Clarke ,  Brett Holmquist ,  Gloria Kwang-Ja Lee ,  Richard E. Reitz

IPC: G01N33/82 ,  G01N30/72

CPC classification number: G01N33/82 ,  G01N30/72 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/00 ,  H01J49/004 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

公开(公告)号：US20180156828A1

公开(公告)日：2018-06-07

申请号：US15848325

申请日：2017-12-20

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Nigel J. Clarke ,  Brett Holmquist ,  Gloria Kwang-Ja Lee ,  Richard E. Reitz

IPC: G01N33/82 ,  G01N30/72 ,  H01J49/00 ,  G01N30/88

CPC classification number: G01N33/82 ,  G01N30/72 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/00 ,  H01J49/004 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

公开(公告)号：US20170102400A1

公开(公告)日：2017-04-13

申请号：US15388844

申请日：2016-12-22

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Nigel J. Clarke ,  Brett Holmquist ,  Gloria Kwang-Ja Lee ,  Richard E. Reitz

IPC: G01N33/82 ,  G01N30/72

CPC classification number: G01N33/82 ,  G01N30/72 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/00 ,  H01J49/004 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

公开(公告)号：US09529004B2

公开(公告)日：2016-12-27

申请号：US15005801

申请日：2016-01-25

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Nigel J. Clarke ,  Brett Holmquist ,  Gloria Kwang-Ja Lee ,  Richard E. Reitz

IPC: G01N33/82 ,  G01N30/72 ,  G01N30/88 ,  H01J49/00

CPC classification number: G01N33/82 ,  G01N30/72 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/00 ,  H01J49/004 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally are directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

公开(公告)号：US08852951B2

公开(公告)日：2014-10-07

申请号：US13751915

申请日：2013-01-28

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Brett Holmquist ,  Nigel J. Clarke ,  Anne Caston-Balderrama ,  Richard E Reitz

IPC: G01N33/00

CPC classification number: G01N27/62 ,  G01N33/82 ,  Y10T436/10 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydroxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

Abstract translation: 提供了使用质谱法检测样品中二羟基维生素D代谢物的存在或量的方法。 方法通常包括在样品中离子化二羟基维生素D代谢物，并检测离子的量以确定样品中维生素D代谢物的存在或量。 在某些优选的实施方案中，所述方法包括在质谱法之前免疫促进二羟维生素D代谢物。 还提供了在单个测定中检测两种或更多种二羟维生素D代谢物的存在或量的方法。

公开(公告)号：US12066446B2

公开(公告)日：2024-08-20

申请号：US18142153

申请日：2023-05-02

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Brett Holmquist ,  Nigel Clarke ,  Anne Caston ,  Richard E. Reitz

IPC: G01N33/82

CPC classification number: G01N33/82 ,  Y10T436/10 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.

公开(公告)号：US11921122B2

公开(公告)日：2024-03-05

申请号：US18107947

申请日：2023-02-09

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Nigel J. Clarke ,  Brett Holmquist ,  Gloria Kwang-Ja Lee ,  Richard E. Reitz

IPC: G01N33/82 ,  G01N30/72 ,  G01N30/88 ,  H01J49/00

CPC classification number: G01N33/82 ,  G01N30/7233 ,  G01N30/72 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2458/15 ,  G01N2560/00 ,  H01J49/00 ,  H01J49/004 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally directed to ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay.

公开(公告)号：US11852636B2

公开(公告)日：2023-12-26

申请号：US18091726

申请日：2022-12-30

Applicant: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED

Inventor： Brett Holmquist ,  Nigel Clarke

IPC: G01N33/82 ,  G01N30/72 ,  H01J49/00 ,  H01J49/14 ,  G01N30/00 ,  G01N30/88 ,  G01N30/02

CPC classification number: G01N33/82 ,  G01N30/7233 ,  H01J49/004 ,  H01J49/145 ,  G01N2030/009 ,  G01N2030/027 ,  G01N2030/8813

Abstract: The invention relates to the detection of non-metabolized vitamin D. In a particular aspect, the invention relates to methods for detecting underivatized non-metabolized vitamin D by mass spectrometry.

公开(公告)号：US11650216B2

公开(公告)日：2023-05-16

申请号：US16748234

申请日：2020-01-21

Applicant: Quest Diagnostics Investments Incorporated

Inventor： Brett Holmquist ,  Nigel Clarke ,  Anne Caston ,  Richard E. Reitz

IPC: G01N27/62 ,  G01N33/82

CPC classification number: G01N33/82 ,  Y10T436/10 ,  Y10T436/20 ,  Y10T436/203332 ,  Y10T436/24

Abstract: Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.