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    Specific detection and quantification of cardiolipin and isolated mitochondria by positively charged AIE fluorogens and method of manufacturing thereof
    26.
    发明授权
    Specific detection and quantification of cardiolipin and isolated mitochondria by positively charged AIE fluorogens and method of manufacturing thereof 有权

    公开(公告)号：US10113968B2

    公开(公告)日：2018-10-30

    申请号：US15100707

    申请日：2014-12-03

    Applicant: The Hong Kong University of Science and Technology

    Inventor： Benzhong Tang ,  Yuning Hong ,  Wai Tung Leung

    IPC: G01N21/64 ,  G01N33/483

    Abstract: The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

    Aggregation induced emission active cytophilic fluorescent bioprobes for long-term cell tracking
    28.
    发明授权
    Aggregation induced emission active cytophilic fluorescent bioprobes for long-term cell tracking 有权
    聚集诱导发射活性细胞色素荧光生物探针进行长期细胞追踪

    公开(公告)号：US09409928B2

    公开(公告)日：2016-08-09

    申请号：US13649819

    申请日：2012-10-11

    Applicant: The Hong Kong University of Science and Technology

    Inventor： Benzhong Tang ,  Ka Ming Ng ,  Qian Luo ,  Yong Yu ,  Yuning Hong ,  Jianzhao Liu ,  Sijie Chen ,  Wing Yip Lam

    IPC: C12Q1/04 ,  C07F7/08 ,  B82Y15/00 ,  G01N21/64

    CPC classification number: C07F7/081 ,  B82Y15/00 ,  G01N21/6458 ,  G01N2021/6439 ,  Y10T428/2982

    Abstract: Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregation-induced emission (AIE) properties, which can be used for long-term cell tracking. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these AIE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.

    Abstract translation: 包含发光体形成的纳米颗粒的荧光生物探针包含具有聚集诱发发射（AIE）性质的发光体，其可用于长期细胞跟踪。 发光体在有机溶液中是非发射的，但当在水溶液中聚集时变得高度发射。 荧光分子可以容易地通过细胞膜，仅染色细胞质，并且在水性介质中形成高度发射的纳米聚集体，而在活细胞中没有任何明显的细胞毒性。 此外，分子可以保留在细胞内，而没有明显的泄漏到外部。 因此，这些基于AIE的化合物可用作选择性和细胞相容性的荧光生物探针，用于长期活细胞跟踪和成像。

    AIEgens for cancer cell imaging
    30.
    发明授权
    AIEgens for cancer cell imaging 有权

    公开(公告)号：US11578265B2

    公开(公告)日：2023-02-14

    申请号：US16319436

    申请日：2017-07-21

    Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

    Inventor： Benzhong Tang ,  Xiujuan Shi ,  Yee Yung Yu ,  Tsz Kin Kwok ,  Zheng Zhao ,  Pengfei Zhang ,  Huifang Su

    IPC: C09K11/06 ,  C07D221/14 ,  C07D213/57 ,  C07D401/12 ,  C07D285/14 ,  A61N5/06 ,  G01N33/533 ,  G01N33/58 ,  C07C211/43

    Abstract: The present subject matter is directed to a luminogen exhibiting aggregation induced emission, wherein T1, T2, and T3 comprise one or more polyynes as a conjugated bridge. The present subject matter is also directed to an AIEgen comprising a hydrophilic pyridium group as a strong electron-withdrawing group; a piperazine group as an electron-donating group; and a α-Cyanostilbene; wherein the AIEgen exhibits aggregation induced emission. The present subject matter is directed to a method of synthesizing an AIEgen and is further directed to a method of labeling comprising incubating a subject having cells with a conjugate formed by conjugating an AIEgen with an antibody; and selectively labeling desired cells by turn-on imaging, wherein labeling occurs when the desired cells are selectively stained by fluorescent emission of the AIEgen upon degradation of the antibody after cellular internalization of the conjugate through endocytosis.

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