公开(公告)号：US12077560B2

公开(公告)日：2024-09-03

申请号：US17211092

申请日：2021-03-24

Applicant: Waters Technologies Corporation

Inventor： Jennifer M. Nguyen ,  Matthew A. Lauber ,  Yun Wang Alelyunas ,  Gregory T. Roman ,  Henry Y. Shion

IPC: B01L3/00 ,  A23C9/146 ,  B01D53/10 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C07K1/18 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/18 ,  G01N21/3577 ,  G01N21/359 ,  G01N21/39 ,  G01N21/45 ,  G01N21/64 ,  G01N30/12 ,  G01N30/68 ,  G01N30/70 ,  G01N30/72 ,  G01N30/88 ,  G01N33/00 ,  G01N33/18 ,  G01N33/50 ,  G01N33/68

CPC classification number: C07K1/18 ,  B01D53/10 ,  A23C9/1465 ,  C12Q2547/101

Abstract: The present disclosure provides devices, systems, kits and methods useful for quantitation of biomolecules such as intact proteins and nucleic acids.

公开(公告)号：US20240141429A1

公开(公告)日：2024-05-02

申请号：US18498328

申请日：2023-10-31

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Catalin Doneanu ,  Matthew A. Lauber ,  Mame Maissa Gaye

IPC: C12Q1/6876 ,  C12Q1/6806

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q2600/158 ,  C12Q2600/166

Abstract: The present disclosure describes methods for analyzing mRNA poly(A) tail sequences with a high resolution to determine poly(A) tail length and heterogeneity. The method involves digesting an mRNA molecule to liberate a poly(A) tail, preparing a chromatographic sample comprising the mRNA poly(A) tails, preparing a second chromatographic sample comprising a reference sequence comprising a mRNA poly(A) tails having a predetermined length, separating the first and second samples by a chromatography method, which result in one or more chromatograms, and determining a sequence length of the mRNA poly(A) tails by comparing the chromatograms of the first and second samples. Chromatography methods for analyzing the poly(A) tail may include ultraviolet size-exclusion chromatography (SEC UV), ultraviolet ion-pair reversed-phase liquid chromatography (IP RP LC UV), ultra high performance liquid chromatography (UPHLC), and combinations thereof.

公开(公告)号：US20230393104A1

公开(公告)日：2023-12-07

申请号：US18206993

申请日：2023-06-07

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Anna Boardman ,  Mathew H. DeLano ,  Matthew A. Lauber

IPC: G01N30/56 ,  G01N30/52 ,  B05D1/00 ,  G01N30/60

CPC classification number: G01N30/56 ,  G01N30/52 ,  B05D1/60 ,  G01N30/6052 ,  G01N2030/567 ,  G01N33/74

Abstract: A device for processing samples is disclosed. Interior surfaces of the device, which come in contact with fluids, define wetted surfaces. A portion of the wetted surfaces are coated with an alkylsilyl coating having the Formula I:




R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20-, —(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

公开(公告)号：US20230393103A1

公开(公告)日：2023-12-07

申请号：US18206989

申请日：2023-06-07

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Mathew H. DeLano ,  Scott A. McCall ,  Jonathan Belanger ,  Theodore A. Dourdeville ,  Kerri Smith ,  Paul Rainville ,  Dimple Shah ,  Stephen J. Shiner ,  Catalin Doneanu ,  Michael Donegan

IPC: G01N30/56 ,  B01J20/32 ,  B05D1/00 ,  B01J20/291 ,  C23C16/04 ,  C23C16/30 ,  C23C14/04 ,  C23C14/12 ,  C23C14/24 ,  G01N30/60

CPC classification number: G01N30/56 ,  B01J20/3272 ,  B05D1/60 ,  B01J20/291 ,  C23C16/045 ,  C23C16/30 ,  C23C14/046 ,  C23C14/12 ,  C23C14/24 ,  G01N30/6052 ,  B05D2518/10 ,  G01N30/7266

Abstract: A device for separating analytes is disclosed. The device has a sample injector, sample injection needle, sample reservoir container in communication with the sample injector, chromatography column downstream of the sample injector, and fluid conduits connecting the sample injector and the column. The interior surfaces of the fluid conduits, sample injector, sample reservoir container, and column form a flow path having wetted surfaces. A portion of the wetted surfaces of the flow path are coated with an alkylsilyl coating that is inert to at least one of the analytes. The alkylsilyl coating has the Formula I:






R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20, -(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or -(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

公开(公告)号：US11747310B2

公开(公告)日：2023-09-05

申请号：US16664403

申请日：2019-10-25

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Michael F. Morris ,  Matthew A. Lauber ,  Darryl W. Brousmiche

IPC: G01N30/00 ,  G01N30/02 ,  B01D15/30 ,  C07D215/48 ,  G01N30/04 ,  C07H15/26 ,  G01N30/88 ,  G01N33/68 ,  G01N30/62

CPC classification number: G01N30/02 ,  B01D15/305 ,  C07D215/48 ,  C07H15/26 ,  G01N30/04 ,  G01N30/88 ,  G01N33/6842 ,  G01N2030/027 ,  G01N2030/047 ,  G01N2030/626 ,  G01N2030/8836 ,  G01N2400/00 ,  G01N2440/38

Abstract: Methods are provided for making rapid labeled dextran ladders and other calibrants useful in liquid chromatography. The methodologies include a two-step process comprising a reductive amination step of providing a reducing glycan and reacting it with a compound having a primary amine to produce an intermediate compound. The intermediate compound is then rapidly tagged with a rapid tagging reagent to produce the rapid labeled dextran ladder.

公开(公告)号：US20230136953A1

公开(公告)日：2023-05-04

申请号：US17976030

申请日：2022-10-28

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Xiaoxiao Liu ,  Michael Donegan

IPC: G01N33/53 ,  G01N33/92 ,  C12Q1/6806

Abstract: The present disclosure provides compositions and methods for sample processing, particularly for oligonucleotide analysis e.g. analysis of formulated nucleic acid drugs. A composition for pretreating at least one target nucleic acid in a biological mixture provided herein includes a chaotropic agent selected from a substituted guanidine, a substituted amidine, a substituted quaternary amine, or a combination thereof, an optional protease, and/or an optional disulfide-reducing agent. Methods of analyzing at least one target nucleic acid in a biological mixture is also provided herein. Furthermore, the present disclosure provides methods for quantifying at least one target cationic lipid interacting with a nucleic acid.

公开(公告)号：US20230126856A1

公开(公告)日：2023-04-27

申请号：US17970962

申请日：2022-10-21

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Darryl W. Brousmiche ,  Jun Yang ,  Weiqiang Gu ,  Mingcheng Xu ,  Peng Chen

IPC: G01N33/68 ,  C07D207/46

Abstract: Provided herein chiral derivatization reagents for use in separating and detecting of amine containing enantiomers. The said chiral derivatization reagents provide a combination of improved detectable properties to facilitate various downstream analyses. In particular, the chiral derivatization reagents include at least one chiral carbon atom; at least one strongly basic moiety; at least one chromophore moiety or at least one fluorophore moiety; and at least one reactive group. The present disclosure further provides methods for analyzing amine-containing enantiomeric isomers using a chromatographic separation device and a mass spectroscopy.

公开(公告)号：US20230111049A1

公开(公告)日：2023-04-13

申请号：US17961061

申请日：2022-10-06

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Xiaoxiao Liu

IPC: C12Q1/6872

Abstract: Methods for quenching a nuclease digestion of a target nucleic acid prior to downstream analysis of the target nucleic acid are disclosed herein. Particularly, methods for controlling the end point of a nuclease digestion prior to sequence analysis of a target nucleic acid is provided. Quenching of a nuclease digestion in the present disclosure employs at least one non-ionic or anionic denaturant combined with an optional reducing agent. The methods presented in this disclosure aids preserving the sample comprising the target nucleic acid or fragments thereof for long term storage and ensures that the effect of contaminating nucleases is eliminated during pretreatment step.

公开(公告)号：US11433320B2

公开(公告)日：2022-09-06

申请号：US16259705

申请日：2019-01-28

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Jennifer M. Nguyen ,  Ximo Zhang ,  Nilini Ranbaduge ,  Robert Birdsall ,  Henry Shion

IPC: B01D15/42 ,  B01D15/32 ,  G01N30/34 ,  B01D15/16 ,  B01J20/283 ,  B01J20/287 ,  G01N30/72 ,  G01N30/84 ,  B01D15/30

Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 0.20% (v/v) difluoroacetic acid and less than about 100 ppb of any individual metal impurity. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

公开(公告)号：US20220080388A1

公开(公告)日：2022-03-17

申请号：US17477340

申请日：2021-09-16

Applicant: Waters Technologies Corporation

Inventor： Yeliz Tunc Sarisozen ,  Nicole L. Lawrence ,  Darryl W. Brousmiche ,  Andrew Wyatt Schmudlach ,  Matthew A. Lauber

IPC: B01J20/289 ,  B01J20/26 ,  B01J20/28

Abstract: The present disclosure is directed to stationary phase materials (e.g., porous inorganic-organic hybrid particles) for performing size exclusion chromatography. Embodiments of the present disclosure feature hydroxy-terminated polyethylene glycol surface modified stationary phase materials.