公开(公告)号：US20090237658A1

公开(公告)日：2009-09-24

申请号：US12421186

申请日：2009-04-09

Applicant: Edward Calvin ,  Wayne D. Roth

Inventor： Edward Calvin ,  Wayne D. Roth

IPC: G01N21/00

CPC classification number: G06K9/6262 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/1465 ,  G01N2015/1497 ,  G06K9/00147 ,  G06K9/6228

Abstract: Methods for altering one or more parameters of a measurement system are provided. One method includes analyzing a sample using the system to generate values from classification channels of the system for a population of particles in the sample. The method also includes identifying a region in a classification space in which the values for the populations are located. In addition, the method includes determining an optimized classification region for the population using one or more properties of the region. The optimized classification region contains a predetermined percentage of the values for the population. The optimized classification region is used for classification of particles in additional samples.

公开(公告)号：US07362432B2

公开(公告)日：2008-04-22

申请号：US11036571

申请日：2005-01-13

Applicant: Wayne D. Roth

Inventor： Wayne D. Roth

IPC: G01J3/30 ,  G01N21/00 ,  G01N21/25 ,  F21V9/16

CPC classification number: B82Y20/00 ,  B82Y10/00 ,  G01N15/14

Abstract: Methods and systems for expanding the dynamic range of a system are provided. One method includes splitting fluorescent light emitted by a particle into multiple light paths having different intensities, detecting the fluorescent light in the multiple light paths with different channels to generate multiple signals, and determining which of the channels is operating in a linear range based on the multiple signals. The method also includes altering the signal generated by the channel operating in the linear range to compensate for the different intensities. Another method includes illuminating a particle in multiple illumination zones with light having different intensities and separately detecting fluorescent light emitted by the particle while located in the multiple illumination zones to generate multiple signals. The method also includes determining which of the signals is located in a linear range and altering the signal located in the linear range to compensate for the different intensities.

Abstract translation: 提供了扩展系统动态范围的方法和系统。 一种方法包括将由颗粒发射的荧光分成具有不同强度的多个光路，检测具有不同通道的多个光路中的荧光以产生多个信号，以及基于以下步骤确定哪个通道在线性范围内操作 多个信号。 该方法还包括改变由在线性范围中操作的通道产生的信号以补偿不同的强度。 另一种方法包括用具有不同强度的光照射多个照明区域中的颗粒，并分别检测位于多个照明区域中的颗粒发射的荧光，以产生多个信号。 该方法还包括确定哪个信号位于线性范围内并且改变位于线性范围内的信号以补偿不同的强度。

公开(公告)号：US07318336B2

公开(公告)日：2008-01-15

申请号：US10918647

申请日：2004-08-13

Applicant: Wayne D. Roth ,  Douglas E. Moore

Inventor： Wayne D. Roth ,  Douglas E. Moore

IPC: G01N21/00

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。

公开(公告)号：US08889347B2

公开(公告)日：2014-11-18

申请号：US11757841

申请日：2007-06-04

Applicant: Wayne D. Roth ,  Charles J. Collins ,  Dung Duong ,  Edward Calvin

Inventor： Wayne D. Roth ,  Charles J. Collins ,  Dung Duong ,  Edward Calvin

IPC: C12Q1/68 ,  C12M1/36 ,  G01N21/00 ,  G01N33/543

CPC classification number: G01N33/54333

Abstract: Systems and methods for performing measurements of one or more materials are provided. One system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels. Another system is configured to image one or more materials in an imaging volume of a measurement device. An additional system is configured to substantially immobilize one or more materials in an imaging volume of a measurement device. A further system is configured to transfer one or more materials to an imaging volume of a measurement device from one or more storage vessels, to image the one or more materials in the imaging volume, to substantially immobilize the one or more materials in the imaging volume, or some combination thereof.

Abstract translation: 提供了用于执行一种或多种材料的测量的系统和方法。 一个系统被配置为将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积。 另一系统被配置成对测量装置的成像体积中的一种或多种材料进行成像。 附加系统被配置为基本上将一种或多种材料固定在测量装置的成像体积中。 另一系统被配置成将一种或多种材料从一个或多个存储容器传送到测量装置的成像体积，以对成像体积中的一种或多种材料进行成像，以使成像体积中的一种或多种材料基本上固定 ，或其某些组合。

公开(公告)号：US20120008869A1

公开(公告)日：2012-01-12

申请号：US13236324

申请日：2011-09-19

Applicant: Wayne D. Roth

Inventor： Wayne D. Roth

IPC: G06K9/62

CPC classification number: G06K9/0014 ,  G01N15/1463 ,  G06T7/0012 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20021 ,  G06T2207/30024 ,  G06T2207/30072

Abstract: Methods, storage mediums, and systems for image data processing are provided. Embodiments for the methods, storage mediums, and systems include configurations to perform one or more of the following steps: background signal measurement, particle identification using classification dye emission and cluster rejection, inter-image alignment, inter-image particle correlation, fluorescence integration of reporter emission, and image plane normalization.

公开(公告)号：US08062609B2

公开(公告)日：2011-11-22

申请号：US11938457

申请日：2007-11-12

Applicant: Jarden E. Krager ,  William R. Deicher ,  Adam Richard Schilffarth ,  Paul Pempsell ,  Wayne D. Roth

Inventor： Jarden E. Krager ,  William R. Deicher ,  Adam Richard Schilffarth ,  Paul Pempsell ,  Wayne D. Roth

IPC: G01N21/01

CPC classification number: G01N15/1404 ,  G01N21/6428 ,  G01N21/76 ,  G01N2015/1409 ,  Y10T137/0352 ,  Y10T137/85986 ,  Y10T436/2575

Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.

Abstract translation: 提供了流式细胞仪，其包括询问流动池和延伸到询问流动池中的多个测定流体线。 操作这种流式细胞计数器的方法包括用鞘液灌注询问流动池，并将不同的分析流体注入穿过多条流体管线的鞘液流。 提供流体线组件，其包括多个毛细管，所述多个毛细管耦合到构造成耦合到流式细胞仪的询问流动池组件的基部。 毛细管的尺寸构造使得当流体线组件布置在流式细胞计数器内并且流体从一个或多个毛细管以相对于询问流动池内的包围护套流体的给定压力差分配时，流体 在询问流动池内基本集中对齐。

公开(公告)号：US08060316B2

公开(公告)日：2011-11-15

申请号：US11461582

申请日：2006-08-01

Applicant: Edward A. Calvin ,  Wayne D. Roth

Inventor： Edward A. Calvin ,  Wayne D. Roth

IPC: G01N33/48

CPC classification number: G01N15/1456 ,  G01N2015/1477 ,  G01N2015/1488 ,  G06F19/707

Abstract: Methods, data structures, and systems for classifying particles are provided. In particular, the methods and systems are configured to acquire a first set of data corresponding to measurable parameters of a microparticle and identify a location of a look-up table to which the first set of data corresponds, wherein the look-up table is framed by values associated with at least one of the measurable parameters. Furthermore, the methods and systems are configured to determine whether the first set of data fits one or more predefined algorithms respectively indicative of a different microparticle classification associated with the identified location of the look-up table. The methods and systems are further configured to classifying the microparticle within at least one predefined categorization based upon the determination of whether the first set of data fits the one or more predefined algorithms.

Abstract translation: 提供了分类粒子的方法，数据结构和系统。 特别地，方法和系统被配置为获取对应于微粒的可测量参数的第一组数据，并且识别第一组数据对应的查找表的位置，其中查找表被框架 通过与至少一个可测量参数相关联的值。 此外，方法和系统被配置为确定第一组数据是否适合分别指示与查找表的所识别的位置相关联的不同微粒分类的一个或多个预定义算法。 所述方法和系统还被配置为基于确定所述第一组数据是否符合所述一个或多个预定义算法来确定所述至少一个预定义分类中的所述微粒。

公开(公告)号：US20100241360A1

公开(公告)日：2010-09-23

申请号：US11461582

申请日：2006-08-01

Applicant: Edward A. Calvin ,  Wayne D. Roth

Inventor： Edward A. Calvin ,  Wayne D. Roth

IPC: G06F19/00 ,  G01N31/00

CPC classification number: G01N15/1456 ,  G01N2015/1477 ,  G01N2015/1488 ,  G06F19/707

Abstract: Methods, data structures, and systems for classifying particles are provided. In particular, the methods and systems are configured to acquire a first set of data corresponding to measurable parameters of a microparticle and identify a location of a look-up table to which the first set of data corresponds, wherein the look-up table is framed by values associated with at least one of the measurable parameters. Furthermore, the methods and systems are configured to determine whether the first set of data fits one or more predefined algorithms respectively indicative of a different microparticle classification associated with the identified location of the look-up table. The methods and systems are further configured to classifying the microparticle within at least one predefined categorization based upon the determination of whether the first set of data fits the one or more predefined algorithms.

Abstract translation: 提供了分类粒子的方法，数据结构和系统。 特别地，方法和系统被配置为获取对应于微粒的可测量参数的第一组数据，并且识别第一组数据对应的查找表的位置，其中查找表被框架 通过与至少一个可测量参数相关联的值。 此外，方法和系统被配置为确定第一组数据是否适合分别指示与查找表的所识别的位置相关联的不同微粒分类的一个或多个预定义算法。 所述方法和系统还被配置为基于确定所述第一组数据是否符合所述一个或多个预定义算法来确定所述至少一个预定义分类中的所述微粒。

公开(公告)号：US20090192746A1

公开(公告)日：2009-07-30

申请号：US12421222

申请日：2009-04-09

Applicant: Edward Calvin ,  Wayne D. Roth

Inventor： Edward Calvin ,  Wayne D. Roth

IPC: G06F17/18 ,  G06F15/00 ,  G01D18/00 ,  G06N5/02

CPC classification number: G06K9/6262 ,  G01N15/1459 ,  G01N15/1463 ,  G01N2015/1465 ,  G01N2015/1497 ,  G06K9/00147 ,  G06K9/6228

Abstract: Methods for altering one or more parameters of a measurement system are provided. One method includes analyzing a sample using the system to generate values from classification channels of the system for a population of particles in the sample. The method also includes identifying a region in a classification space in which the values for the populations are located. In addition, the method includes determining an optimized classification region for the population using one or more properties of the region. The optimized classification region contains a predetermined percentage of the values for the population. The optimized classification region is used for classification of particles in additional samples.

Abstract translation: 提供了用于改变测量系统的一个或多个参数的方法。 一种方法包括使用系统分析样品以从样品中的颗粒群体的系统的分类通道产生值。 该方法还包括识别分类空间中的群体的值所位于的区域。 此外，该方法包括使用区域的一个或多个属性来确定群体的优化分类区域。 优化的分类区域包含对于群体的值的预定百分比。 优化的分类区域用于附加样品中颗粒的分类。

公开(公告)号：US07523637B2

公开(公告)日：2009-04-28

申请号：US11947116

申请日：2007-11-29

Applicant: Wayne D. Roth ,  Douglas E. Moore

Inventor： Wayne D. Roth ,  Douglas E. Moore

IPC: G01N21/00

CPC classification number: G01N15/1404 ,  G01N15/1012 ,  G01N21/645 ,  G01N2015/1415 ,  G01N2015/1477 ,  G01N2201/12746

Abstract: Various methods for controlling one or more parameters of a flow cytometer type measurement system are provided. One embodiment includes monitoring parameter(s) of the measurement system during measurements of sample microspheres. The method also includes altering the parameter(s) in real time based on the monitoring. Another method includes monitoring a temperature proximate to the measurement system. One such method includes altering a bias voltage of an avalanche photo diode in response to the temperature using empirically derived data. A different such method includes altering output signals of a photomultiplier tube in response to the temperature using a characteristic curve. Some methods include monitoring a temperature of a fluid, in which sample microspheres are disposed, that will flow through the flow cytometer type measurement system. This method also includes determining a velocity of the sample microspheres in the measurement system from a viscosity of the fluid at the temperature.

Abstract translation: 提供了用于控制流式细胞仪型测量系统的一个或多个参数的各种方法。 一个实施例包括在样品微球的测量期间监测测量系统的参数。 该方法还包括基于监视实时更改参数。 另一种方法包括监测靠近测量系统的温度。 一种这样的方法包括使用经验导出的数据来响应于温度来改变雪崩光电二极管的偏置电压。 不同的这种方法包括使用特性曲线响应于温度改变光电倍增管的输出信号。 一些方法包括监测将流过流式细胞仪类型测量系统的流体样品微球的设置温度。 该方法还包括从该温度下的流体的粘度确定测量系统中的样品微球的速度。