公开(公告)号：US10078078B2

公开(公告)日：2018-09-18

申请号：US15210634

申请日：2016-07-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B03C1/02 ,  B03C1/30 ,  B03C5/00 ,  G01N33/561 ,  B03C1/28 ,  C12N15/10 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

公开(公告)号：US10078066B2

公开(公告)日：2018-09-18

申请号：US14636646

申请日：2015-03-03

Applicant: Rafael V. Davalos ,  Hadi Shafiee ,  Michael Benjamin Sano ,  John L. Caldwell

Inventor： Rafael V. Davalos ,  Hadi Shafiee ,  Michael Benjamin Sano ,  John L. Caldwell

IPC: G01N27/447 ,  B03C5/00 ,  B03C5/02 ,  B01D57/02

CPC classification number: G01N27/44791 ,  B01D57/02 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26

Abstract: Devices and methods for performing dielectrophoresis are described. The devices contain sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.

公开(公告)号：US20180257088A1

公开(公告)日：2018-09-13

申请号：US15908161

申请日：2018-02-28

Applicant: RITTER GMBH

Inventor： FRANK RITTER

IPC: B03C1/28 ,  B01L9/06

CPC classification number: B03C1/288 ,  B01L3/5025 ,  B01L9/06 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2400/043 ,  B03C1/0332 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26 ,  G01N35/0098

Abstract: A magnetic separation rack made of a plastics material for performing automated magnetic separation processes comprises a base plate in which a matrix of m×n wells is formed, and a comb-like hollow rod plate with a matrix of m×n hollow rods each connected with its upper end to a horizontal carrier plate and having a radially outwardly protruding ring collar on its lower end region. The carrier plate has a recess on the position of placement of each hollow rod, the extension of the recess corresponding at least to the projection of the ring collar onto the carrier plate. Each hollow rod has on its outer circumference a plurality of radial ribs extending along the axis of the hollow rod towards the upper end starting from the ring collar, and their connecting the hollow rod to the carrier plate is in one piece while extending radially through the recess.

公开(公告)号：US10073079B2

公开(公告)日：2018-09-11

申请号：US14783494

申请日：2014-04-11

Applicant: The Governing Council of the University of Toronto

Inventor： Shana Olwyn Kelley ,  Edward Hartley Sargent ,  Mohamad Reza Mohamadi

IPC: G01N33/49 ,  B03C1/01 ,  B03C1/033 ,  B03C1/034 ,  B03C1/28 ,  B03C1/30 ,  G01N15/00 ,  G01N15/06 ,  B01L3/00 ,  G01N33/543 ,  G01N33/574 ,  G01N27/00 ,  G01N1/40

CPC classification number: G01N33/491 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/01 ,  B03C1/0335 ,  B03C1/034 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/4077 ,  G01N15/00 ,  G01N15/0606 ,  G01N15/0656 ,  G01N27/00 ,  G01N33/54326 ,  G01N33/57434 ,  G01N2015/0053 ,  G01N2015/0065

Abstract: Devices and methods for capture of target particles in a flow. There is a plurality of flow rate-reducing structures in a flow chamber, each structure including a trapping surface shaped to reduce flow rate in a vicinity of the trapping surface. Reduced flow rate in the vicinity of the trapping surface is non-zero and has a magnitude lower than that of flow rate in other regions of the flow chamber. The reduced flow rate is sufficiently low for an attraction force acting on the target particles to overcome drag force on the target particles, to promote capture of particles in the vicinity of the trapping surface. The device may exhibit different sorting zones for capturing particles that experience different amounts and/or types of attraction force. The device may enable sorting of cells according to their level of display of specific protein surface markers.

公开(公告)号：US20180250686A2

公开(公告)日：2018-09-06

申请号：US14914917

申请日：2014-08-28

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T CHIU ,  Robbyn K. PERDUE ,  Eleanor S. JOHNSON

IPC: B03C5/00 ,  C12N13/00 ,  B01L3/00 ,  G01N27/447 ,  G01N27/27 ,  B03C5/02

CPC classification number: B03C5/005 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/06 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0424 ,  B01L2400/0487 ,  B03C5/026 ,  B03C2201/26 ,  C12M33/00 ,  C12M47/04 ,  C12N13/00 ,  G01N15/0612 ,  G01N15/1459 ,  G01N15/1463 ,  G01N27/27 ,  G01N27/44756 ,  G01N35/08 ,  G01N2015/1006 ,  G01N2015/149

Abstract: Methods, systems, and devices for manipulating objects are provided. In certain aspects, the methods, systems, and devices can be used for dielectrophoretic manipulation of objects using bipolar electrodes. Some aspects of the methods, systems, and devices of the present disclosure can be used for encapsulation and amplification of samples.

公开(公告)号：US10016765B2

公开(公告)日：2018-07-10

申请号：US15414039

申请日：2017-01-24

Applicant: National Chiao Tung University

Inventor： Ching-Yao Chen ,  Yan-Hom Li ,  Ren-Chiuan Shiu

IPC: B03C1/24 ,  C02F1/48 ,  B01L3/00 ,  A61M37/00

CPC classification number: B03C1/24 ,  A61M37/00 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/20 ,  B03C2201/26 ,  C02F1/48

Abstract: A method for advancing a non-magnetically responsive functional agent includes steps (a) and (b). In step (a), a magnetically responsive agent construct at a target site is subjected to a homogeneous static magnetic field such that magnetically responsive micro particles are aligned to obtain a magnetic chain while the non-magnetically responsive functional agent is separated and discrete from the magnetic chain. In step (b), the magnetic chain is subjected to a rotational magnetic field to cause repeating breaking up and reformation of the magnetic chain such that the non-magnetically responsive functional agent is displaced to a predetermined position.

公开(公告)号：US10006840B2

公开(公告)日：2018-06-26

申请号：US14358922

申请日：2012-11-20

Applicant: Miltenyi Biotech GmbH

Inventor： Burgund Kauling ,  Volker Huppert

IPC: A01N1/02 ,  G01N33/00 ,  C12N5/071 ,  G01N1/34 ,  B01D21/00 ,  C12Q1/02 ,  C12N5/00 ,  B03C1/01 ,  B03C1/28 ,  B01D21/26

CPC classification number: G01N1/34 ,  B01D21/00 ,  B01D21/0009 ,  B01D21/262 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12N5/0087 ,  C12Q1/02

Abstract: The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed.

公开(公告)号：US09981273B2

公开(公告)日：2018-05-29

申请号：US14042585

申请日：2013-09-30

Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： Mehdi Javanmard ,  Sam Emaminejad ,  Janine Mok ,  Michael N. Mindrinos

IPC: B03C7/02 ,  B81B1/00 ,  B03C5/00 ,  B03C5/02

CPC classification number: B03C7/023 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  B81B1/006

Abstract: The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.

公开(公告)号：US09968943B2

公开(公告)日：2018-05-15

申请号：US15199842

申请日：2016-06-30

Applicant: UNITED ARAB EMIRATES UNIVERSITY

Inventor： Saud A. Khashan

IPC: B03C1/00 ,  B03C1/025 ,  B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26

Abstract: The magnetic particle separator uses an induced magnetic field to separate magnetic particles held in solution by magnetophoresis. The magnetic particles may be, for example, inherently paramagnetic or superparamagnetic, may be magnetically tagged or the like. First and second magnetic particles initially flow along a longitudinal direction. An external magnetic field along a lateral direction, orthogonal (or near orthogonal) to the longitudinal direction, is applied to an externally magnetizable wire, which extends along a transverse direction orthogonal to both the longitudinal and lateral directions. The external magnetic field generates an induced magnetic field in the externally magnetizable wire, and the induced magnetic field generates repulsive magnetic force on the first and second magnetic particles. Due to differing magnetic susceptibility, size and/or mass between the first and second magnetic particles, they are separated by following separate paths generated by the respective magnetic forces thereon.

公开(公告)号：US20180093280A1

公开(公告)日：2018-04-05

申请号：US15720548

申请日：2017-09-29

Applicant: ARKRAY, Inc.

Inventor： Masahiro Kozuka

IPC: B03C1/28

CPC classification number: B03C1/288 ,  B03C1/01 ,  B03C2201/18 ,  B03C2201/26

Abstract: Provided is a method for separating particles, a separation apparatus, and a separation system.