公开(公告)号：US10755905B2

公开(公告)日：2020-08-25

申请号：US11718235

申请日：2005-10-28

Applicant: Yongdong Wang ,  Ming Gu

Inventor： Yongdong Wang ,  Ming Gu

IPC: G01N30/72 ,  H01J49/00 ,  G06K9/00 ,  G01N33/68 ,  B01D59/44

Abstract: A method for analyzing data from a mass spectrometer comprising acquiring raw profile mode data containing one or more ions and their isotopes in a mass spectral range; calculating theoretical isotope distributions for all ions of interest including native or labeled ions based on their molecular composition; convoluting the theoretical isotope distributions with target peak shape function specified during instrument calibration, actual peak shape functions, or approximated peak shape functions, to obtain theoretical isotope profiles for all ions; constructing a peak component matrix of relevant theoretical isotope profiles included as peak components; performing a weighted multiple linear regression between the profile mode data and the peak component matrix; and reporting regression coefficients as relative concentrations for each of the ions, or ranking these ions based on fitting statistics as search results. A mass spectrometer system (FIG. 1) operating in accordance with the method. Medium having computer code for operating the spectrometer.

公开(公告)号：US08819512B1

公开(公告)日：2014-08-26

申请号：US12357304

申请日：2009-01-21

Applicant: Yongdong Wang ,  Hao Zhuang

Inventor： Yongdong Wang ,  Hao Zhuang

IPC: G08C25/02

CPC classification number: H04L1/08 ,  H04L49/552 ,  H04L69/163 ,  H04L69/166

Abstract: A computer-implemented method for detecting TCP packet losses and expediting packet retransmission is disclosed. The method includes assigning to each packet transmitted or retransmitted a sequential order number; determining from an ACK packet or SACK segment thereof the sequential order number of a received packet; recording the determined sequential order number as a highest order number in case the determined sequential order number is greater than a current received order number, and retransmitting all unacknowledged packets having sequential order numbers less than the highest received order number.

Abstract translation: 公开了一种用于检测TCP分组丢失并加快分组重传的计算机实现的方法。 该方法包括分配发送或重传的每个分组的顺序订单号码; 从ACK分组或其SACK分段确定接收分组的顺序序号; 在确定的顺序号大于当前接收到的订单号码的情况下，将所确定的顺序号码记录为最高订单号码，并且重新发送具有小于最高接收订单号的顺序订单号码的所有未确认的包。

公开(公告)号：US07670848B2

公开(公告)日：2010-03-02

申请号：US11251873

申请日：2005-10-18

Applicant: Michael R. Gambini ,  John C. Voyta ,  John Atwood ,  Susan A. Atwood-Stone, legal representative ,  Bruce E. DeSimas, II ,  Edward Lakatos ,  Jeff Levi ,  Israel Metal ,  George Sabak ,  Yongdong Wang

Inventor： Michael R. Gambini ,  John C. Voyta ,  John Atwood ,  Bruce E. DeSimas, II ,  Edward Lakatos ,  Jeff Levi ,  Israel Metal ,  George Sabak ,  Yongdong Wang

IPC: G01N21/76 ,  G01T1/10

CPC classification number: G01N21/763 ,  G01N21/15 ,  G01N21/274 ,  G01N21/276 ,  G01N21/31 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/76 ,  G01N33/581 ,  G01N2021/6471 ,  G01N2333/90241 ,  Y10T436/115831

Abstract: In a luminescence detecting apparatus and method for analyzing luminescent samples, luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, an infrared filter, and a camera lens, whereupon a focused image is created by the optics on the camera. The use of an infrared filter suppresses stray IR radiation resulting from plate phosphorescence (which can result in abnormally high backgrounds and/or alteration of the image received by the camera).

Abstract translation: 在用于分析发光样品的发光检测装置和方法中，将发光样品放置在托盘中的多个样品孔中，并将该托盘放置在包含电荷耦合器件相机的可见光不透过室中。 在室中，来自发光样品的光通过准直仪，菲涅尔场透镜，红外滤光镜和照相机镜头，由相机上的光学器件产生聚焦图像。 使用红外滤光器可抑制板状磷光产生的杂散红外辐射（可能导致摄像机接收的图像异常高的背景和/或变化）。

公开(公告)号：US07656521B2

公开(公告)日：2010-02-02

申请号：US12119239

申请日：2008-05-12

Applicant: Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

Inventor： Yongdong Wang ,  Bernhard H. Radziuk ,  David H. Tracy

IPC: G01J3/00

CPC classification number: G01J3/28 ,  G01J3/42

Abstract: In a spectroscopic process a sample for producing a test spectral line or spectrum of at least one component contained in the sample is stimulated and the transmitted and/or emitted electromagnetic rays are used to create the test spectral line or spectrum. In order to improve such a spectroscopic process to such an extent that variations of certain parameters, which alter the shape and/or occurrence of a spectral line, are compensated, a comparison spectral line or spectrum of a known comparison material is produced under substantially the same parameters as the sample. The comparison spectral line or spectrum is compared with an ideal comparison spectral line or spectrum in order to calculate a transfer function, andthe transfer function is applied to the test spectral line or spectrum in order to calculate a corrected test spectral line or spectrum.

Abstract translation: 在分光过程中，刺激用于产生测试谱线或样品中包含的至少一种组分的光谱的样品，并且使用透射和/或发射的电磁波来产生测试光谱线或光谱。 为了改进这种光谱过程，使得补偿了改变光谱线的形状和/或出现的某些参数的变化，已知比较材料的比较光谱线或光谱基本上在 与样品相同的参数。 将比较谱线或频谱与理想的比较谱线或频谱进行比较，以计算传递函数，并将传递函数应用于测试谱线或频谱，以便计算校正的测试谱线或频谱。

公开(公告)号：US07493225B2

公开(公告)日：2009-02-17

申请号：US10576564

申请日：2004-10-20

Applicant: Yongdong Wang ,  Ming Gu

Inventor： Yongdong Wang ,  Ming Gu

IPC: G01R35/00

CPC classification number: G06K9/00496 ,  H01J49/0009 ,  H01J49/0027

Abstract: A method for obtaining at least one calibration filter for a Mass Spectrometry (MS) instrument system. Measured isotope peak cluster data in a mass spectral range is obtained for a given calibration standard. Relative isotope abundances and actual mass locations of isotopes corresponding thereto are calculated for the given calibration standard. Mass spectral target peak shape functions centered within respective mass spectral ranges are specified. Convolution operations are performed between the calculated relative isotope abundances and the mass spectral target peak shape functions to form calculated isotope peak cluster data. A deconvolution operation is performed between the measured isotope peak cluster data and the calculated isotope peak cluster data after the convolution operations to obtain the at least one calibration filter. Provisions are made for normalizing peak widths, combining internal and external calibration, and using selected measured peaks as standards. Aspects of the methods are applied to other analytical instruments.

Abstract translation: 一种用于获得用于质谱（MS）仪器系统的至少一个校准滤波器的方法。 对于给定的校准标准，获得质谱范围内的测量同位素峰聚类数据。 对于给定的校准标准，计算与之对应的同位素的相对同位素丰度和实际质量位置。 规定了在各个质谱范围内的质谱目标峰形函数。 在所计算的相对同位素丰度和质谱目标峰形状函数之间进行卷积运算，以形成计算的同位素峰集群数据。 在卷积运算之后，在所测量的同位素峰集群数据与计算出的同位素峰集群数据之间进行解卷积运算，以获得至少一个校准滤波器。 规定峰值宽度归一化，组合内部和外部校准，并使用选定的测量峰值作为标准。 方法的各个方面适用于其他分析仪器。

公开(公告)号：US20060156792A1

公开(公告)日：2006-07-20

申请号：US11249722

申请日：2005-10-12

Applicant: Yongdong Wang

Inventor： Yongdong Wang

IPC: G01N30/02 ,  G01N30/90

CPC classification number: G01N30/463 ,  G01N30/466 ,  G01N30/80 ,  G01N30/82

Abstract: A multi-dimensional separation system having parallel traps for effluent from prior separation dimension and parallel latter separation columns, the latter columns being coupled to the traps. At least one trap enriches components of effluent while at least one other trap is releasing trapped components to a detector, which may be a mass spectrometer. Internal standards may be provided, as in a release solvent, for the calibration of one of the chromatographic columns and the detection system. The system may comprise a multiple channel selector for multiple streams, wherein all of the streams flow at the same time.

Abstract translation: 一种多维分离系统，其具有用于来自先前分离尺寸和平行的后分离柱的流出物的平行捕集器，后者的塔联接到捕集器。 至少一个陷阱丰富了流出物的组分，而至少一个其他捕集器将捕获的组分释放到可以是质谱仪的检测器。 可以提供内部标准，如在释放溶剂中，用于校准色谱柱和检测系统之一。 该系统可以包括用于多个流的多信道选择器，其中所有流同时流动。

公开(公告)号：US06983213B2

公开(公告)日：2006-01-03

申请号：US10689313

申请日：2003-10-20

Applicant: Yongdong Wang

Inventor： Yongdong Wang

IPC: G01R35/00 ,  H01J49/00

CPC classification number: G06K9/00496 ,  H01J49/0009 ,  H01J49/0027

Abstract: There is provided a method for obtaining at least one calibration filter for a Mass Spectrometry (MS) instrument system. Measured isotope peak cluster data in a mass spectral range is obtained for a given calibration standard. Relative isotope abundances and actual mass locations of isotopes corresponding thereto are calculated for the given calibration standard. Mass spectral target peak shape functions centered within respective mass spectral ranges are specified. Convolution operations are performed between the calculated relative isotope abundances and the mass spectral target peak shape functions to form calculated isotope peak cluster data. A deconvolution operation is performed between the measured isotope peak cluster data and the calculated isotope peak cluster data after the convolution operations to obtain the at least one calibration filter.

Abstract translation: 提供了一种用于获得用于质谱（MS）仪器系统的至少一个校准滤波器的方法。 对于给定的校准标准，获得质谱范围内的测量同位素峰聚类数据。 对于给定的校准标准，计算与之对应的同位素的相对同位素丰度和实际质量位置。 规定了在各个质谱范围内的质谱目标峰形函数。 在所计算的相对同位素丰度和质谱目标峰形状函数之间进行卷积运算，以形成计算的同位素峰集群数据。 在卷积运算之后，在所测量的同位素峰集群数据和计算出的同位素峰集群数据之间进行解卷积运算，以获得至少一个校准滤波器。

公开(公告)号：US06518068B1

公开(公告)日：2003-02-11

申请号：US09621961

申请日：2000-07-21

Applicant: Michael R. Gambini ,  John C. Voyta ,  John Atwood ,  Bruce E. DeSimas, II ,  Edward Lakatos ,  Jeff Levi ,  Israel Metal ,  George Sabak ,  Yongdong Wang

Inventor： Michael R. Gambini ,  John C. Voyta ,  John Atwood ,  Bruce E. DeSimas, II ,  Edward Lakatos ,  Jeff Levi ,  Israel Metal ,  George Sabak ,  Yongdong Wang

IPC: G01N2164

CPC classification number: G01N21/763 ,  G01N21/15 ,  G01N21/274 ,  G01N21/276 ,  G01N21/31 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/76 ,  G01N33/581 ,  G01N2021/6471 ,  G01N2333/90241 ,  Y10T436/115831

Abstract: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents. Preferred embodiments of processing software integrated with the invention include elements for mechanical alignment, outlier shaving, masking, manipulation of multiple integration times to expand the dynamic range, crosstalk correction, dark subtraction interpolation and drift correction, multi-component analysis applications specifically tailored for luminescence, and uniformity correction.

Abstract translation: 公开了一种用于分析发光样品的发光检测装置和方法。 将发光样品放置在托盘中的多个样品孔中，并且将托盘放置在包含电荷耦合器件照相机的可见光不透水室中。 样品可以注入孔中，样品可以通过注射器注入缓冲液和试剂。 在室中，来自发光样品的光通过准直仪，菲涅尔场透镜，滤光器和照相机镜头，由此电荷耦合器件（CCD）照相机上的光学器件产生聚焦图像。 使用菲涅耳场透镜与准直器和滤光器结合使得样品之间的串扰降低到现有技术可获得的水平以下。 发光检测装置和方法的优选实施例包括所有操作，多波长滤光轮和样品和试剂的机器人处理的中央处理控制。 与本发明集成的处理软件的优选实施例包括用于机械对准，异常剃刮，掩蔽，操纵多个积分时间以扩展动态范围，串扰校正，暗减法内插和漂移校正的元件，专门用于发光的多分量分析应用 ，均匀性校正。

公开(公告)号：US06430513B1

公开(公告)日：2002-08-06

申请号：US09479642

申请日：2000-01-07

Applicant: Yongdong Wang ,  David H. Tracy ,  Paul G. Saviano ,  Alan M. Ganz ,  Koichi Nishikida ,  Gitesh Kumar

Inventor： Yongdong Wang ,  David H. Tracy ,  Paul G. Saviano ,  Alan M. Ganz ,  Koichi Nishikida ,  Gitesh Kumar

IPC: G01N3100

CPC classification number: A61B5/6814 ,  A61B5/14546 ,  A61B5/14553 ,  G01N21/49

Abstract: Constituents such as oxy- and deoxy-hemoglobin are monitored non-invasively in an animal organ such as a brain with a spectrometric instrument by passing radiation through the organ. Concentrations are computed from the spectral intensities and from a statistical correlation model. To predetermine the correlation model, the procedures are effected for a plurality of organs of a same type with each organ having established concentrations of the selected constituents, and the correlation model is statistically determined from the concentrations and corresponding intensities. For more accuracy computations are normalized to path length which may be determined by utilizing several discrete wavelengths with RF modulations.

Abstract translation: 通过使辐射通过器官，通过光谱仪器在诸如脑的动物器官中非侵入性地监测诸如氧和血红蛋白的成分。 浓度从光谱强度和统计相关模型计算。 为了预先确定相关模型，对于具有确定的所选组分浓度的每个器官，对于多个相同类型的器官进行该程序，并且根据浓度和相应的强度统计地确定相关模型。 为了更准确的计算，归一化到可以通过利用具有RF调制的几个离散波长来确定的路径长度。

公开(公告)号：US06418383B1

公开(公告)日：2002-07-09

申请号：US09503092

申请日：2000-02-11

Applicant: Yongdong Wang

Inventor： Yongdong Wang

IPC: G06F1582

CPC classification number: G01J3/433 ,  G01J3/28 ,  G01N21/274 ,  G01N21/31

Abstract: An iterative method and apparatus for correction and compensation of analytical signals, such as spectrometric data, is provided which corrects for spectral cross-talk; compensates for spectral shift; and reduces error propagation. The method and apparatus can be applied to a multicomponent sample analysis using least squares procedure with differentiation while reducing noise propagation.

Abstract translation: 提供了用于校正和补偿分析信号（如光谱数据）的迭代方法和装置，其校正了频谱串扰; 补偿光谱偏移; 并减少错误传播。 该方法和装置可以应用于使用具有微分的最小二乘法的多组分样本分析，同时降低噪声传播。